流空

The CFD method was applied to calculate and simulate the form and action process of the cavitation at the representative character of the cavitation erosion on the surface of the hydraulic turbines.Some argumentation was presented that micro jet impacted the wall with a certain degree when bubble collapsed with the result that the maximal strain of the wall annularly distributed the local of the boundary touching the micro jet.Cavitation was gradual to develop regionally at the mode of chain reaction and di...

针对水轮机叶片表面气蚀的典型特征，利用CFD方法进行仿真计算，模拟气蚀形成和作用过程；根据模拟计算结果提出了新观点：空泡溃灭发生微射流以一定的倾角冲击壁面，壁面材料产生的最大应变在流束边界局部区域，呈环状分布；空泡气蚀在局部区域是渐进的，以"连锁反应"形式扩展，具有一定过流环境的分布特征；典型的冲蚀缺陷形貌是空泡气蚀在不同阶段的产物。

With Gongboxia Hydropower Station on the Yellow River taken as an example,some related problems were discussed,including the key points on the shape optimization of horizontal and vertical rotary internal energy dissipator,t.

以黄河公伯峡水电站为例，论述了竖井旋流式与水平旋流式内消能工在体型优化中的重点与方向问题；泄流能力的协调与匹配问题；通气孔的设计、消能率的构成、空化空蚀、衬砌标准与范围、下游水深的影响等问题。

With a cavitating model established for N-S equations from cavitation theory, cavitation evolution on the surface of a hydrofoil is developed: incipience of cavity, cavity growing, reentrant flow appearing, cavity retracting, shedding and smaller broken cavities moving downwards with the main flow.

根据空化流原理，建立了基于N-S方程求解的空化模型，利用这个模型计算了空化在翼型表面的演化过程：初生、长大、形成回注水流、空穴回缩、减小、脱落，以及小空泡随主流向下游运动的现象。

A calculation model is established for the corrugation and annular flow field of a stratification injection allocator by using 3D Max and it is put into VR environment of PHOENICS and based on the actual geometric size of the allocator,κ-ε turbulent calculating model is used for numerical calculation of water medium flow field by using orthogonal and detail grid under coordinate system.

用3D Max建立了分层配注器波纹环空流场的计算模型，调入PHOENICS的VR编辑环境中，根据实际配注器的几何尺寸，在柱坐标系下，采用局部加密正交网格，应用k-ε湍流计算模型对水介质流场进行了数值计算，给出流场的速度分布与压力分布曲线，并对比分析了3种形状的波纹环空流场的数值计算结果。

In order to research the mechanism and effect of hydrodynamic cavitational enhancement, find the basic rule which affected it. The work has been carried out as following.First, an effective equipment of hydrodynamic cavitition is built. Second, we find a new method to capture the free radicals, which is used to measure the amount of hydroxyl. And its correlation with the intensity of cavitation has been studied aims at analyzing the effect of various factors on the yield of hydroxyl, achieving the optimum hydrodynamic cavitation conditions. Then, hydrodynamic cavitation is used to the degradation of organic wastewaters containing rhodamine B, and sterilization of biologic wastewaters containing enterobacter aerogenes. Finally, numerical simulation based on computer fluid dynamic has been made for verifying the effect of hydrodynamic cavitation theoretically.

为了探求水力空化强化效应的机制和效果，寻求影响空化强化效应的基本规律，本论文就此开展了如下研究工作：设计建立实用有效的水力空化实验装置；建立空化自由基捕捉新方法，并以此定量检测水力空化羟自由基，研究空化羟自由基产量与空化强度的关系，研究各种因素对空化自由基产量的影响，以寻求最佳空化强化条件；在此基础上，进行了水力空化对含罗丹明B有机废水降解处理、对含产气肠杆菌生物污水杀菌处理研究，实验检验水力空化的强化效果；根据计算流体力学理论对空化流场进行数值模拟，从理论上验证水力空化的发生效果。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

At the same time, it first uses hydromechanical coupling theory to analyze the influence of groundwater during disposal, and contributes to promote further study of abandoned stope disposal.

同时，在采空场处理研究领域，首次运用渗流应力耦合理论分析了地下渗流对采空场处理的影响，为深入开展采空场处理研究发挥了重要作用。

Based on the characteristics of casing annulus gas-venting wells, the particularity of multi-phase flow in the condition that the casing-head gas flows through a nearly stagnant liquid was investigated. Based on the theory of Taylor bubble, a model to calculate void fraction and pressure gradient in various viscosity liquids was developed. This model is applicable to bubbly flow and slug flow.

以泰勒气泡在黏滞流体中的上升流速作为主要依据，建立了不同油黏度下套管环空放气流动的截面含气率和压降计算模型，并提出了不同黏度、不同气油比的套管定压放气井环空流动计算及套管压力分布的多相流模型，该模型主要适用于泡流和段塞流。

A high-speed camera is used to visualize the flow structures,and Laser Doppler Velocity is employed for velocity measurement.The osculations are conducted on the two-dimensional hydrofoil section,based on a single-fluid model of the cavitation:the liquid/vapor mixture is considered as a homogeneous fluid whose eompsition is regulated by mass tranfer equation.The RNG k-ε turbulence model with modified eddy viscosity coefficient is used for computing,and the coefficient is related to the vapor and liquid densities in cavitated regions for simulating the cavitating flow.

实验采用高速录像技术观察了云状空化随时间的结构变化，应用 LDV 测量了云状空化条件下翼型周围的速度分布；采用 N-S 方程和基于空泡动力学方程的空化模型计算了绕水翼云状空化流场，湍流模型采用了 RNG k-ε方程，为了改善空化流动的计算精度，引入了一种与空化区域水汽相密度相关的系数，对湍流涡黏性系数进行了修正。

The osculations are conducted on the two-dimensional hydrofoil section,based on a single-fluid model of the cavitation:the liquid/vapor mixture is considered as a homogeneous fluid whose eompsition is regulated by mass tranfer equation.The RNG k-ε turbulence model with modified eddy viscosity coefficient is used for computing,and the coefficient is related to the vapor and liquid densities in cavitated regions for simulating the cavitating flow.

实验采用高速录像技术观察了云状空化随时间的结构变化，应用 LDV 测量了云状空化条件下翼型周围的速度分布；采用 N-S 方程和基于空泡动力学方程的空化模型计算了绕水翼云状空化流场，湍流模型采用了 RNG k-ε方程，为了改善空化流动的计算精度，引入了一种与空化区域水汽相密度相关的系数，对湍流涡黏性系数进行了修正。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。