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But ECT technology has the following characteristics: 1 the measured capacitance and its change induced by the measured material concentration variation are very small, but the stray capacitances of co-axial screening cable and CMOS switches are relative very large, so the capacitance detecting is easily affected by the stray capacitances, 2 the electric fields in the sensors detecting region is affected by the permittivity distribution, non-uniformity of the space responsive sensitivity is very high, and the image reconstruction is undetermined.

但由于ECT技术中,首先所检测的微电容量及其因物流相含率的变化所引起的变化量很小,而由相关的连接同轴屏蔽电缆与切换CMOS开关所引入的杂散电容远远大于测量电容,微电容的测量容易受杂散电容等的影响;其次,多电极电容传感器测量区内的电场受介质分布的影响,且测量区内的空间响应灵敏度极不均衡,其影响图像重建的不确定性;其三,ECT中的图像重建,由于其是一个欠定问题的求解,在迭代求解方法中,迭代的初值影响迭代过程收敛到近似解的速度;其四,对于气/固两相流应用而言,由于ECT系统在线标定的难以实现,管壁磨损等因素的影响制约该技术应用于生产工艺中气/固两相流的长期连续检测。

The current for pantograph shall be increased especially for the metro vehicle equipped with the third rail current collector.

需要增加受电弓受流,特别是对采用第三轨受流器的地铁列车。

We established an effective method to forecast and optimize the control of power supply on the two-dimensional separations of microchip capillary electrophoresis, using the relationship of electroosmosis and electricity.

本课题利用电渗流与电流的关系建立了一套直接有效的对二维微流控分析芯片的电源控制方案进行预测和初步优化的方法。

Air is heated in electric heater through the fan, then goes through the gas distributor into the fluidized bed dryer; and material enters the fluidized through the top. Materials keep fluidized in virtue of the flotage effect of the hot air. Energy and mass exchange in the fluid bed dryer.

流化床干燥过程:空气由鼓风机引入电加热器,加热后经布风板进入流化床,物料由流化床顶部预先加入,通过调整进风量,使物料在热空气的作用下保持流化状态,实现物料的流化干燥。

And then we proved that Kirchhoff law can be applied to describing traffic flow characteristic.

在此基础上,证明了基尔霍夫定律在网络交通流描述中的适用性,从而运用完善的电路网理论来分析了道路网交通流特性。

The above mentioned multiple LED bulbs, current limiting resistor and the connection point of down-leads in series are plugged into the above multiple transverse holes of core accordingly

多个LED灯泡,通过LED灯泡导电电脚上连接的引线相互串联、及与至少一个限流电阻相串接,该LED串联灯串的首端和末端的引线与上述芯线中的铜合线作电气连接,所述的多个LED灯泡、限流电阻及其引线的串联连接点相庆地塞入上述芯线中的多个横向孔中

The results show that the duration of the 3 phases during the culture of cell suspension is related to the inoculum size, the initial nutrient concentration, the feeding of nutrients and the culture conditions such as temperature and dissolved oxygen, that the maximum cell yield reaches about 12g/L for batch culture or 35g/L for fed-batch culture in dry weight, that the saponin content of cells quickly increases in the bloom phase and keeps on increasing until the early stage of the declining phase, with a maximum saponin content of about 60~70g/kg in dry weight, that the conductance value of cell suspension decreases from more than 8000μS to less than 600μS with the growth of cells, but increases when the cells autolyze, and that part of cells turns red in the later culture, which has no apparent effect on the saponin content.

结果显示:细胞3个生长时期的持续时间与接种量、初始营养浓度、营养流加情况及培养条件有关;分批培养和流加培养时的最大细胞得率分别为12g/L和35g/L;细胞的皂甙含量在旺盛生长期快速上升,并保持增长至衰退期前期;干细胞中最大的皂甙含量达60~70g/kg;随着细胞的生长,培养液的电导值从8000μS以上下降至600μS以下,细胞自溶后逐渐回升。另外,培养后期部分细胞颜色变红,但没有发现颜色转变对皂甙含量造成影响。

Methods From March 1992 to January 2005, there were 395 cases undergoing LCDET, involving choledochotomy, electrohydralic lithothipsy, dilation stenosis, stent placement and T-tube drainage.

从1992年3月~2005年1月,运用腹腔镜胆总管探查T管引流术的手术方式,包括胆总管切开,胆管镜取石,液电碎石,扩张狭窄,放置支架,T管引流,对395例病人进行治疗。

Zeta potential,density of surface carboxyl groups,the interaction between carboxyl groups and component in copolymerization emulsion,mechanical properties of films,state of water,particle diameter and distribution of particle diameter and theological behavior of emulsion were investigated by Zeta meter,the conductometric titration, particle size apparatus,dynamic mechanics analysis,differential scanning calorimmetry,scanning electron microscope,rheological apparatus,respectively, to study the mechanism on the water resistance of modified PVAc emulsion.

利用Zeta电位仪、电导滴定法、激光粒度仪、DMA、DSC、SEM、流变仪等手段从Zeta电位、表面羧基密度、羧基和乳液组分中的相互作用、羧酸改性后胶膜的力学性能、水的存在状态、粒径分布、流变性能和微观形貌方面探索了羧基提高乳液耐水性的机理。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank,选择包含人主要T细胞抗原表位序列的人PSCA基因片段,应用异种化抗原设计技术,保留人T细胞抗原表位,设计异种化PSCA融合抗原片段;(2)根据核酸序列按中心模板法设计引物,应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因,以PCR、限制性酶切和DNA序列测定法进行鉴定:(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点,采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4),并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中,转染293T细胞,借助免疫荧光+流式细胞术考察插入片段表达效率,最终选定PSCA_3片段进行下一步研究;(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下,插入pVAX1-neo-IRES—GM/B7载体中,构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB,并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况;(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞,制备小鼠前列腺癌模型,并采用股四头肌肌肉注射+电脉冲法(Electroporation,EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB,同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照,通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率,来评价该DNA疫苗在小鼠体内的抑瘤效果。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。