流电

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

The male and female rats (n=15, respectively) were randomly divided into three groups, and Fluoro-Ruby, a fluorescent tracer, was stereotaxically injected into the ventromedial hypothalamus, bed nucleus of the stria terminalis and posterodorsal nucleus of the amygdale by iontophoresis, respectively.Five days later, the rats were perfused, and the sections containing SDN-POA region and site of injection were cut on a freezing microtome.the sections were viewed to assess extent of tracer deposition in the injection site and to reveal the EGFP or Fluoro-Ruby fluorescence in SDN-POA under fluorescence microscope.

雌、雄性大鼠(各15只)随机各分成3组，分别通过离子电渗法将示踪剂Fluoro-Ruby注入下丘脑腹内侧核（VMH、终纹床核、杏仁核后背侧核。5 d后，灌流固定鼠脑，做冷冻冠状切片，荧光显微镜下确定示踪剂注入位置，并观察SDN-POA绿色荧光和Fluoro-Ruby标记荧光。

A new method was reported for the determination of tanshinol and protocatechuic aldehyde in dripping solution by microfluidic chip with contactless conductivity detection.

建立了微流控芯片非接触电导检测法测定丹参滴注液中丹参素与原儿茶醛含量的分析方法。

In this thesis, a pulled bare capillary tip was proposed to be used as the ESI emitter. The outside surface of the tip was neither coated with electric-conductivity materials nor subjected to an external high voltage. Thus, there is no concern such as short lifetime that generally observed in conventional sheathless tips.

本论文尝试直接利用高温拉制及氢氟酸处理过的毛细管尖端做为电喷洒尖端，此设计不需要涂布导电材料及施加第二组电压於毛细管尖端处，此无鞘流界面因此能够避免因导电材料涂布不密合稳定而造成的易脱落及寿命短等问题。

The product qualities,safety,transportation,storage,useful deposition ratios,deposition rates,one pass conversions,deposition temperatures,electrical energy consumption and manufacturing costs are summarized for silane,dichlorosilane,trichlorosilane and tetrachloro silane method s.

对三氯氢硅法、四氯化硅法、二氯二氢硅法和硅烷法生产的多晶硅质量、安全性、运输和存贮的可行性、有用沉积比、沉积速率、一次转换率、生长温度、电耗和价格进行了对比；对还原或热分解使用的反应器即钟罩式反应器、流床反应器和自由空间反应器也进行了比较。

Glycerol trioleate was used as the dispersant and its content in the slip was also optimized.

流延成型法是目前制备熔融碳酸盐燃料电池电极板的主要工艺。

Temperature ununiformity induced pressure gradient and changed variation behavior of applied electric field in microchannel.

热效应会对电渗流速度场产生影响，并诱导压强梯度和改变外电场在微通道的变化特征。

As MEMS (Micro Electro-Mechanical Systems) technology progresses rapidly, the synergism between MEMS and biomedical technology becomes closer than ever before. When the uTAS was introduced in the last two decades, many microfluidic chips, microelectrodes, and microreactors are being studied for drug detection, environment detection, gene analysis etc. Due to its small volume, low pollution, low consumable and good portability, uTAS plays a very important role in biomedical, biochemical, environment engineering fields.

随着MEMS(Micro Electro-Mechanical System，微机电系统)制造技术的不断发展，生物医学与其之间的联系越来越紧密，自上世纪90年代初提出uTAS(Micro Total Analysis System，微全分析系统)之后，各种各样的微流控芯片、微反应器、微电极、微分离器等便大量涌现，广泛应用于药物检测、环境监测、基因分析等诸多领域中，并且由于其试剂消耗量少、检测效率高、环境污染小、体积小、易于携带等优点，在生物医学、化学、环境工程等领域受到越来越多的重视。

The results show that, as black carbon with particleshape, steel fiber with high aspect ratio and density does not reach to the vadose zone in higher content, their mechano-electric effect is not obvious.

研究结果表明：钢纤维长径比小，密度大，和粒子状的碳黑一样，在很高掺量仍处于未渗流区，两种水泥石的力电耦合效应都不明显。

This research is the Fluid-structure coupling analysis of piezoelectric valveless micropump.

本研究为模拟压电无阀式微帮浦流固耦合分析。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。