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Nucleus changes in apoptotic cells were investigated by using Hoechst 33258 staining and photofluorography. Apoptotic rate was evaluated by propidium iodide staining and flow cytometer.

应用Hoechst 33258染色法观察凋亡细胞的形态学改变;PI染色流式细胞仪检测细胞凋亡率;Western印迹法检测Bcl-2蛋白的表达。

CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型;以硫氢化钠作为H2S的供体;应用CCK-8比色法检测细胞存活率;碘化丙啶染色流式细胞技术检测细胞凋亡率;Hoechst33258染色检测细胞凋亡的形态学变化;罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位;2',7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

Based on the theoretical of ice slurry phase flow and heat transfer, established a heat transfer model of ice slurry flowing in horizontal pipes, at same time analyzed the effects of flow and ice packing fraction on heat transfer of fan and coil

在传统液固两相流的研究成果基础上,建立起二元冰在水平管内换热的数学模型,同时针对二元冰在风机盘管中的输送情况,分析了输送速度、含冰率对于二元冰换热的影响

After overcoming the flux shift during the process, the production ability of strains could be the limiting factor for further increasing the production. To avoid the random modification on strains, the comprehensive enzymatic study of nucleoside producing-pathway was conducted on four strains with different producing ability. And the results could be used to direct further reconstruction of strains. It was also found that nucleotidase was of much importance to nucleoside production.

在成功克服了代谢流的迁移之后,菌株的代谢能力可能成为了进一步提高产率的限制因素,为了避免对生产菌的进一步改造中的盲目性,对四株不同菌株间嘌呤途径关键酶活力的系统测定,对肌苷、鸟苷生产菌种的酶学特征有了全面的了解;并发现了核苷降解酶对于核苷生产能力至关重要;酶学特性研究对肌苷、鸟苷生产菌的进一步改造提供了方向。

Methods: According to their urinary albumin excretion rate, Diabetes mellitus patients with micrangium disease were further divided into three subgroups: normo-albuminuria、micro-albuminuria and clinical proteinuria; The expression of platelet membrane α-glucoprotein (CD62p) and cytolysosome protein (CD63)、platelet aggregation function and mean platelet volume was determined with flow cytometer、blood aggregation analyzer、automated blood cell counter (MEK-6318K) respectively, and compared with 30 normal controls.

对糖尿病微血管病变患者按尿清蛋白排泄率分为正常清蛋白尿组、微量清蛋白尿组、临床清蛋白尿组三组,分别用流式细胞术、血液聚集仪、全自动血细胞分析仪测定血小板内α-颗粒膜蛋白(CD62p)、溶酶体蛋白(CD63)的表达及血小板聚集功能和平均体积,并与30例正常对照组比较。

This flow chamber allows realtime observation and record of the interaction between platelets and vWF under welldefined shear rate.This approach can be applied to platelet adhesion research.

本研究方法可以在精确剪切率和不同流态的流体环境条件下,实时地观察、记录血小板与vWF相互作用的情况,为血小板各种生理功能的研究以及相关药物的研究应用创造条件。

123 cases of brain concussion patients were divided into Citicoline group(59cases)and Nimodipine group(64 cases) randomly, middle cerebral artery,basilar arterywere monitored by 3DTCD at the 3rd,6th and 9th day respectivelyThe results were compar...

将 1996年 2月至 1999年 10月治疗的 12 3例脑震荡患者分成胞二磷胆碱组( 5 9例),尼莫地平组( 6 4例),于住院 2 4小时,3、 6、 9天采用 3D- TCD监测大脑中动脉,基底动脉脑血流平均峰流速度,并与对照组进行比较,结合治疗后临床症状消失率、治愈率进行综合分析。

MAIN OUTCOME MEASURES: Nerve growth factor expression rate was determined in Schwann cells by using flow cytometry.

主要观察指标:流式细胞仪检测许旺细胞神经生长因子的表达率。

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