流率

Abstract］ objective to study the chronic subdural haematoma pathological mechanism characteristic,curing method and factor failing.methods the method carries on a clinical research to 217 sufferers and the whole set goes skull ct to scan.for the very first time surgical operation method all for drill a hole to lead to drainage.results cured 193 cases (88.94%),haematoma residual 9 cases (4.15%),haematoma recrudesces 15 cases (6.91%).conclusion procedure selection should be based on condition of the patients with hematoma,drainage completely and anti-pneumo encephalos may important.

摘要］ 目的探讨分析慢性硬膜下血肿的病理特点、治疗方法和失败因素。方法对217例患者进行临床研究，全组均行头颅ct扫描。首次手术方式均为钻孔引流术。结果恢复良好者193例（88.94%），血肿残留9例(4.15%)，血肿复发15例(6.91%)。结论对慢性硬膜下血肿的治疗应根据病情选择手术方式，术中血肿冲洗要彻底及术中术后要预防气颅的发生，可降低术后血肿复发率。

Methods The lyophilized platelets were rehydrated after being preserved 30 days. The change of morphology and ultrastructure of platelets was observed under the light microscope and transmission electron microscope. The survival rate and apoptosis rate of platelets were analyzed by flow cytometry. The aggregation rates of rehydrated lyophilized platelets were measured by using 1 U/mL thrombin.

以新鲜血小板为对照，以保存30d后再水化的冻干血小板为实验对象，分别在倒置相差显微镜和透射电镜下观察血小板的细胞形态和超微结构，用流式细胞仪检测血小板凋亡率，并以1×10^3U/L凝血酶为诱导剂检测再水化血小板的聚集活性。

The litter decomposition and processing rate among three microhabitats in the upper Chichiawan Stream were significantly different. It was highest in riffles and lowest in pools.

桃山西溪的枯落叶片分解率在不同微栖地之间有差异，从快而慢分别是：浅濑区＞深流区＞深潭区。

Multiphase reaction engineering (530.2440);RPD;gas holdup;hot sparged;gas-liquid-solid three- phase system;stirred tank

多相流反应工程(530.2440)；通气功率；气含率；热态通气；气-液-固三相体系；搅拌槽

There was significant difference in frequency of cells in frontal drainage system between sinusitis group and control group, including terminal cell, anterior ethmoid cell, agger nasi cells, and perifrontal cells.

两组间额窦引流通道内出现并影响额窦通气引流功能的部分气房（终末气房、前筛气房、鼻丘气房和额气房）出现率的差异有显著性。

When the best asphalt content of the mixture used in asphalt stabilized macadam base is determined through new Marshall Experiment,stability value and flow value can be used as detection data,some volume index such as density,voidage and saturation are the main design basis.

在通过大马歇尔试验确定沥青稳定碎石基层混合料最佳沥青用量时，稳定度和流值可作为检测检验数据，密度、空隙率和饱和度等体积指标则是主要的设计依据。

Non-cohesive bedload; steady current; asymmetric waves; plane bed; sheet flow; theoretical model; transport rate formula; flume experiment; numerical experiment

非粘性推移质；恒定流；非对称波浪；动平床；临底高含沙层；理论模型；输沙率公式；水槽试验；数值试验

The simulation results show that the packet block rate of the modified adaptive assembly algorithm is only about one tenth of that of adaptive assembly algorithm.

仿真结果表明：在相同的调度算法下，更能降低业务流的自相似性，分组阻塞率降低一个数量级。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

At the same time the strong turbulence and swirl in the chamber speed up the combustion process which leads to a efficient fuel consumption and improves its smoke.

利用缸内产生的挤流和涡流加快了扩散燃烧过程，使油耗率降低，烟度有了较大的改善。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。