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FCM analysis showed that the apoptotic rate of MDA-MB-231 cells and intracellular Ca(superscript 2+) concentration increased gradually with the increase of deguelin concentration. RT-PCR and Western blotting analysis showed that the expression of Bcl-2 mRNA and protein was clown-regulated and that of Sax was op-regulated after deguelin treatment.

流式细胞术检测显示细胞凋亡率和细胞内钙离于浓度随鱼藤素浓度的增加而增多;RT-PCR和蛋白印迹检测结果发现鱼藤素能使Bcl-2 mRNA和蛋白表达减少,而Bax表达增加。

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞,用100 TCID50柯萨奇B3病毒(CVB3)感染心肌细胞,建立病毒性心肌炎实验模型,然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞,观察心肌细胞的形态和搏动,用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白,四唑蓝比色法测定心肌细胞活性和病毒抑制率,流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

The linear relationship between the reciprocal of electroosmotic mobility and the square root of concentration was derived by mathematical method.

通过数学方法推导出电渗流迁移率的倒数与缓冲液浓度的平方根之间存在线性关系。

We find that using the guide shell and the cooling cover improves the argon flow field,the solid-liquid interface,and the temperature gradient.It is good for the emanatory of crystallization latent heat and the improvement of the radical resistivity in the crystal.

研究发现在导流系统中引入导流筒及冷却功能后,氩气流场得到明显的改善,晶体中纵向温度梯度均匀性改善,固液界面趋于平坦,有利于结晶潜热的散发和单晶径向电阻率的均匀性。

According to constant distortion criterion, sliding window rate allocation method is proposed for fine granularly coded bitstream.

根据恒定失真准则,本文首次提出了适合精细可分级编码码流的滑动窗口码率分配方法(SlidingWindow Rate Allocation,SWRA)。

Results There were spontaneous apoptosis in the cultured granulosa cells and ceramide could signi-ficantly increase apoptosis (P<0.05).En hanced apoptotic surf was observed by flow cytometry.

结果 体外培养的颗粒细胞存在自发性凋亡,神经酰胺处理的颗粒细胞凋亡率明显高于对照组(P<0.05);流式细胞术分析可见增强的凋亡峰。

The simulated results show that compared with the multi-nozzle gasifier,the gasifying flow field and the effective gases in the new gasifier is superior.In the gasifier of same capacity,the efficiency of carbon conversion of the new gasifier is more about 0.75%,and percent hydrol...

结果表明,新型水煤浆气化炉在炉内气化流场的组织和产出的有效气成分上均优于多喷嘴对置式水煤浆气化炉,在相同容量的气化炉内,新型水煤浆气化炉与多喷嘴对置式水煤浆气化炉对比,碳转化率高0.75%,水解率高近2.91%,干有效气成分高5.34%,冷煤气效率高7.09%,每产出1 000 m3有效气(CO+H2)可节省氧气20 m3,节省煤36 kg。

These indicates bioactive peptides in Chinese forest frog skins have great potential for the exploitation and utilization. S_(180) cells were inoculated hypodermically at the concentration of 1×10~6 cells per mouse and the bioactive peptides in Chinese forest frog skins were resolved into aseptically distilled water to make injection liquid. After injected continuously 15 days, the inhibition rate of tumor was calculated. The induction of tumor cell apoptosis of active peptides in Chinese forest frog skins could be detected by morphological

将S_(180)胃肉瘤细胞接种至小鼠皮下,选取中国林蛙皮肤生物活性肽不同浓度样品液,连续注射种瘤小鼠15天后,计算抑瘤率;采用透射电镜观察细胞形态学变化、DNA梯度琼脂糖凝胶电泳技术、MTT比色法、流式细胞检测等技术观察中国林蛙皮肤生物活性肽对K562肿瘤细胞凋亡过程的诱导。

Keywords combine;separated things of concave;distribution law;rock- intervallic flow rolled drum;smash rate

联合收割机;凹板分离物;分布规律;齿排间隔组合式轴流脱粒滚筒;破碎率

The proliferation of SGC-7901 cell line was markedly inhibited by shRNA-GC-CⅢ( P .05). There was changes in cell morphology, such as cell volume reduction, karyopyknosis and chromatin conglomeration. FCM showed that GC-C inhibition caused the appearance of sub-G 0 /G 1 peak which increased from 5.47%(48h after transfection) to 5.63%(72h after transfection).

转染GC-C shRNA真核表达载体后SGC-7901细胞的增殖能力受到抑制( P 。05);细胞形态发生改变,细胞体积缩小,核固缩,染色质浓缩聚集于核膜;流式细胞术检测显示G 1 峰前出现亚二倍体峰,转染48、72h后凋亡率分别为5.47%和5.63%( P nbsp; 0.05)。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。