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Morphological and flourescent changes were assessed using confocal microscopy in whole-mount organ of Corti preparations. Results (1) After the animals were exposed to broadband noise at 122 dB SPL in 4 h/day for 2 days, both apoptosis and necrosis appeared in OHCs. The single strand DNA in apoptotic OHCs were observed both in guinea pigs and mice.(2) In normal OHCs, EndoG was distributed outside of nuclei. EndoG translocated from outside to inside of the nuclei in both apoptotic and necrotic OHCs following noise exposure.(3) The MNNG cochlear perfusion and noise exposure both caused the transloctation of AIF from the mitochondria to the nuclei. The translocation of AIF took place in both apoptotic and necrotic OHCs.

结果 (1)暴露于120 dB SPL的白噪声环境中每天4小时,连续2天后引起豚鼠和小鼠耳蜗外毛细胞凋亡时,其细胞核内产生ssDNA,而在正常细胞内没有三ssDNA;(2)在正常情况下,EndoG分布于耳蜗毛细胞的细胞核外,在暴露于上述噪声后发生凋亡和坏死的豚鼠耳蜗外毛细胞中,EndoG从细胞核外转移到细胞核内,细胞核中的EndoG显著增加;(3)豚鼠耳蜗外淋巴灌流烷化剂MNNG后发生耳蜗外毛细胞凋亡和坏死,在凋亡和坏死的耳蜗外毛细胞中,AIF自线粒体转移到细胞核,其变化与噪声损伤引起耳蜗外毛细胞凋亡和坏死时一致。

Methods: The conventional intracellular recordings were conducted in MNs of spinal cord slices isolated from neonatal rats (8~14 days old). Observed in MNs were the effects of TRH on excitatory postsynaptic potentials evoked by contralateral ventrolateral funiculus stimulation i.e.

应用新生大鼠(8~14d龄)脊髓切片MN细胞内记录技术,TRH灌流给药,观察其对cVLF电刺激诱发的兴奋性突触后电位的影响。

Met hod: Hypertrophy of prostate model rat was established by injecting t estosterone to gelding male rats. After being treated with Qianlie Huichun ig 30 days, the rats were killed and prostate glands were resected for examination. The Fas expressio n was examined by immunobistochemical SABC.

采用大鼠去势后注射丙酸睾酮引起前列腺增生法造模,灌胃给药 30d后,处死大鼠取前列腺组织并称湿重,用免疫组化方法检测前列腺组织中Fas阳性细胞的表达率,用流式细胞术检测细胞凋亡率和观察细胞凋亡峰。

MethodsThe rat single-pass intestinal perfusion technique was applied, and the gravimetry was used to correct the perfusion volume.

方法采用大鼠在体肠单向灌流吸

Objective To observe the effect of muscone on the blood-brain harrier and neuropathological changes after complete cerebral ischemia/reperfusion of rats.

目的 观察麝香酮对大鼠完全性脑缺血后再灌流期间血脑屏障及神经病理改变的影响。

Objective To study the effects of hemoperfusion on hemodynamics and serum concentration in the patients with severe alcohol intoxication.

目的研究血液灌流对严重酒精患者血流动力学和血中乙醇浓度的影响。

Objective To study the change of hydroxyl free radicals during cerebral ischemia and reperfusion and the effect of ketamine on it.

目的 研究脑缺血和再灌流期间的羟自由基变化及氯胺酮对其影响。

BACKGROUND: Inadequate hyperventilation may trigger cerebrovascular contraction and lead to lowered cerebral perfusion and oxygen supply-demand imbalance.

背景:不适当的过度通气可能诱使脑血管收缩,导致脑低灌流和氧供需失衡。

RESULTS摘要: Under isotonic conditions, no single channel activities were observed in most patches. Hypotonic superfusioninduced cell swelling resulted in the appearance of active channel. Under symmetrical condition, the current induced by hypotonicity showed outward rectification and an unitary conductance of (38.1±2.5)pS.

结果摘要:等渗状态下,用细胞贴附式记录,多数心肌细胞膜片无通道活动,用低渗溶液灌流细胞,在细胞容积增大的同时,出现单通道电流的激活。

Hypotonic superfusioninduced cell swelling resulted in the appearance of active channel. Under symmetrical condition, the current induced by hypotonicity showed outward rectification and an unitary conductance of (38.1±2.5)pS.

结果: 等渗状态下,用细胞贴附式记录,多数心肌细胞膜片无通道活动,用低渗溶液灌流细胞,在细胞容积增大的同时,出现单通道电流的激活。

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