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Methods In situ single pass perfusion model was used and the concentrations of three components in perfusate were determined by HPLC in combination with diode array detection.

运用单向灌流模型并采用HPLC法同时测定莪术油中三成分在灌流液中的浓度。

The concentration of monomethyihydrazine in brain increased along with perfusion time and monomethylhydrazine concentration in the perfusate.

MMH灌流浓度分别为145,290和580mgL^(-1),灌流时间为2,5,8及10min;采用对-二甲氨基苯甲醛比色法进行脑组织中的MMH浓度测定。

The SWS-2000 blood perfusion machine, which can perfuse and filter blood, mainly provides power and monitoring for extracorporeal blood circulation, and secure the life from poison, food or medicine intoxication and acute left heart failure.

项目简介 SWS-2000型血液灌流机能够做血液灌流和血液滤过,主要是提供血液体外循环动力及对体外循环进行监控,可抢救各种毒物、药物、食物中毒和急性左心衰等病症。

In the first 2-3 hours there is always a strengthening of contraction, while after 5-10 hours, when the heart gives much feebler contraction due to exhaustion, this beneficial effect of phosphatide tends to disappear.

二葡萄糖在灌流液中的作用视室温与离体时间而定。在较高室温长期灌流而衰竭的心脏,葡萄糖是必要的,它可以维持心肌收缩并被心肌所消耗。如心脏离体时间较短,室温较低,则葡萄糖并非必需。

Objective: To detect the expression of SAMDC-mRNA in the cortex and subcortex of rat after the different time of the reperfusion following 2h focal cerebral ischemia by occlusion of MCA in order to explore the variety and signification of SAMDC-mRNA in this condition.

目的:检测大鼠MCA区缺血再灌流后不同时相脑皮质及皮质下中的SAMDC-mRNA的表达,探讨其在缺血再灌流后的变化及意义。

The process of cerebral hypoxia was simulated by giving superfusion with ACSFOGD for 14 minutes in the control and test groups. The propofol test group was subdivided into P1, P5 and P15 groups to undergo superinfusion with ACSF containing 1, 5 and 15μmolL^(-1) propofol respectively.

缺氧组:脑片用缺氧无糖的人工脑脊液灌流14min,实施缺氧过程;药物组分为P1、P5和P15组:在ACSFOGD灌流液中分别含1,5和15μmolL^(-1)的丙泊酚。

Methods By means of whole-cell patch clamp technique, the epileptiform discharge model of rats hippocampal CA1 neurons was constructed with extracellular perfusion of 0.5 μmol/L veratridine, and the model should be regarded as successfully estabilshed if bursting discharge emerged within 30 min perfusion.

通过膜片钳全细胞记录技术,胞外灌流0.5μmol/L藜芦碱,制作大鼠海马CA1锥体神经元癫痫样放电模型,如果在灌流30min内出现振荡样放电,即认为造模成功。

Acidemia and hypotension developed in rats following declamping of aorta. However, pretreatment with glibenclamide did not significantly affect the systemic blood pressure following reperfusion acidosis.

另外,在大白鼠的主动脉阻断后再灌流会发生酸血症以及低血压,然而先使用glibenclamide治疗并没有办法明显的改善再灌流后因酸血症而造成的低血压。

Results: The light microscope pictures of group A(no [α-32P] ATP in the perfusate) showed that there was no specially labeled silver pellet in or out of rat liver cell. But the light microscope pictures of group B (37 MBq [α- 32P]ATP in the perfusate) showed that distribution of numerous [α-32P]ATP autoradiographic silver pellets in rat liver cell, and no autoradiographic silver pellet was found in the hepatic sinus and vessel.

结果:灌流液中不加[α-32P]ATP的A组光镜照片显示大鼠肝细胞内外均未见特异性标记银粒显影,而灌流液中含37 MBq[α-32P]ATP的B组光镜照片显示大鼠肝细胞内可见多个大小不等的[α-32P]ATP自显影银粒分布,而肝窦及肝小叶中央静脉等血管内则几乎无黑色自显影银粒分布。

Results The mean value of DPOAE decreased from 41.3 dB to 13.7 dB after perfusion of fluid devoid of calcium ion, it revived to 36.7 dB after reperfusion with normal perilymphatic fluid.

结果 用无钙人工外淋巴灌流后DPOAE的均值从41.3 dB SPL降至13.7 dB SPL,再用正常钙浓度的外淋巴灌流后又可回升到36.7 dB SPL。

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