流明

Each of them was percutaneously drained under ultrasound guidance and flushed with sensitive antibiotic every day.

明确诊断后，在B超引导下行经皮穿刺脓肿引流和对脓腔进行抗菌素灌洗。

In 0. 1 mmol/L Ca2+,glutamate-isolated P 1Q components,both photopic and scotopic,are enhanced,suggesting that the differential effects of low calcium on the horizontal cells must occur during signal transferring from photoreceptors to horizontal cells.

在0。 1 mmol/L Cal+ Ringer液灌流时，无论是明视PI或暗视PI，其反应的幅度均增大，提示发生在低钙时Cone-HC和Rod-HC光反应性的不同变化产生于突触传递的过程中。

In order to clear heterogeneous reservoirs waterflooding performance, and decreased interlayer and within-layer contradictions, and improved the water flooding recovery, experimental study should be conducted first, This paper based on Pucheng Sasan reservoir sandstone cores to study object. Multi-point pressure measurement of water flooding oil experimental apparatus was established. Through the three-layer water flooding experiment to study injection strategy, interlayer interference and recovery factors. True sandstone micro-model water flooding used newly developed color visual image analysis system researched water-drive types, forms of residual oil and waterflood efficiency. Percolation mathematical model was established, black oil simulation program was developed, long-core displacement experimental results were matched, pressure and saturation change were researched by the simulation matching results.

为了明确非均质储层注水开发动态过程，减轻由于层间和层内非均质造成的开发矛盾，提高水驱采收率，本文从实验研究出发，以濮城沙三储层岩心为研究对象，建立了一套带多测压点的多层长岩心水驱油实验装置，通过开展三层水驱油实验，研究注水方式、层间干扰以及采收率影响因素；真实砂岩微观模型水驱油实验采用最新研制的彩色可视化图像分析系统研究水驱类型、残余油形成类型以及水驱油效率；建立并求解渗流数学模型，编制黑油模拟程序，对长岩心驱替实验进行拟合，通过拟合结果研究驱替过程中油水饱和度和压力的变化情况等研究工作。

Teck said the combined company would have the advantage of a diversified base, reducing earnings and cash flow volatility.

特克-科明科公司表示，合并后的公司将拥有多样化业务的优势，并能够减少收益和现金流方面的波动性。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

In China,O'Leary says,'instead of using resources to count every case, the authorities are focusing efforts on treatment and prevention,' in line with the WHO's recommendations on emphasizing prevention and monitoring of H1N1 trends.

兰睿明说，在中国，有关当局没有利用现有资源去统计每一桩病例，而是将相关举措集中在治疗和预防上，这符合世卫组织重点对甲流趋势进行防控的建议。

Aerators are widely used in flood discharge tunnel s and spillways to prevent the cavitation damage of the structures.

对高水头、大单宽流量、低佛氏数、小底坡长明流泄洪洞，在反弧段末端采用常规的掺气坎往往难以取得理想的掺气效果，同时，反弧段下游边墙易出现空蚀破坏。

The company developed the production of "the new card" series of ventilation refrigeration equipment with high efficiency, energy saving, low-noise and other excellent properties, has formed 21 series, more than 1000 kinds of specifications, its main products have dedicated transformer cooling fans, various kinds of dedicated air-conditioning fan, fire smoke extraction fans, high-performance mixed-flow fan, blower pipes, roof fans, all kinds of centrifugal fans, material handling fans, as well as refrigeration and air coolers evaporative condensers and so on, are widely used in subway, tunnels, machinery, automobile , chemical, building, metallurgy, light industry, refrigeration, boilers, papermaking, pharmaceutical and other industries.

本公司研制开发生产的&明新牌&系列通风制冷设备，具有高效、节能、低噪等优良特性，已形成二十一个系列，一千多种规格，其主要产品有变压器冷却专用风机、各种空调专用风机、消防排烟风机、高效混流风机、管道风机、屋顶风机、各种离心风机、物料输送风机以及制冷用蒸发式冷凝器及冷风机等，广泛应用于地铁、隧道、机械、汽车、化工、建筑、冶金、轻工、制冷、锅炉、造纸、医药等行业。

5 Those hours that with gentle work did frame The lovely gaze where every eye doth dwell Will play the tyrants to the very same, And that unfair which fairly doth excel: For never-resting time leads summer on To hideous winter and confounds him there, Sap checked with frost and lusty leaves quite gone, Beauty o'er-snowed and bareness every where: Then were not summer's distillation left A liquid prisoner pent in walls of glass, Beauty's effect with beauty were bereft, Nor it nor no remembrance what it was.

五 那些时辰曾经用轻盈的细工织就这众目共注的可爱明眸，终有天对它摆出魔王的面孔，把绝代佳丽剁成龙锺的老丑：因为不舍昼夜的时光把盛夏带到狰狞的冬天去把它结果；生机被严霜窒息，绿叶又全下，白雪掩埋了美，满目是赤裸裸：那时候如果夏天尚未经提炼，让它凝成香露锁在玻璃瓶里，美和美的流泽将一起被截断，美，和美的记忆都无人再提起：但提炼过的花，纵和冬天抗衡

Compares the economy of energy and heating charges for two heating seasons in the Kaili Garden, Tianjin, chosen as a pilot project of heating metering, where a calorimeter is installed at each building, a balance valve at each entrance and an evaporative heat meter and a thermal static valve on each radiator in the one-pipe vertical heating system, analyses the existed problems and energy saving potentials both by users' behaviour and by technical measures, considers the heating charging system reform and establishment of related regulations to be urgently needed.

辛坦选择天津凯立花园住宅小区作为热计量试点工程，在单管顺流式热水供暖系统中，每栋楼装一个热能表，每个热力入口装热量调节阀；每个散热器入口装温控阀，散热器上装蒸发式热分配表。对两个供暖季的节能效果与取暖费节省情况进行了对比分析，总结了存在的问题，从行为节能和技术节能的角度指出了节能的潜力，认为尽快将取暖费由暗补变明补、制定热计量收费管理办法是两个亟待解决的问题。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新