流化

Isolation, cultivation and identification of human embryonic NSC from cerebral cortex of 7-12 week abortus. By using flow cytometry and MTT assay, the effects of various concentration of TSPG and TSPG cooperating with cytokines in NSC culture media for 3 days on proliferation of human embryonic NSC has studied. By employing immunocytochemistry assay of the expression of tyrosine hydroxylase, the effect of different dilution of TSPG and TSPG cooperating with IL-1 on induced differentiation of human embryonic NSC into dopaminergic neuron has researched.

从7～12周人工流产胚胎脑组织中分离培养人胚胎NSC，免疫细胞化学鉴定NSC特异性nestin抗原表达，在NSC的培养体系中加入TSPG，采用流式细胞术和MTT法检测不同浓度TSPG以及TSPG与EGF，bFGF联合应用对人胚胎NSC增殖的影响；经免疫细胞化学检测培养细胞酪氨酸羟化酶表达，分析不同浓度TSPG以及TSPG与IL-1联合应用对NSC定向诱导为DA能神经元的影响。

The process industry such as the treatment of the preparation of stock of the roast with the aerification that sheds voice to change a technology to already was applied extensively at coal and combustion, catalytic cracking, mineral petrolic, grain, raw material.

流态化技术已被广泛应用于煤的气化和燃烧、石油的催化裂化、矿物的焙烧、颗粒物料的制备、生物质的加工等过程工业。

For satisfactory fluidization under these conditions an agitator is used in the bed.

在这些情况下，为了得到满意的流态化，在流化床中加有搅拌器。

Methods:To produce antihuman DR5 monoclonal antibody(mDRA6) by immunizing BALB/c mice using DR5 protein. The change of DR5 expression after DDPtreated on HL60 cells was detected by FACS. Morphologic changes of HL60 cells were observed under fluorencence microscope. Cytotoxic and apoptotic effects of mDRA6 and DDP on HL60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.

DR5蛋白免疫BALB/c小鼠，融合筛选抗DR5杂交瘤细胞，制备抗DR5单抗——mDRA6；流式细胞术测定顺铂对HL60细胞表面DR5表达及细胞凋亡率；荧光显微镜下观察mDRA6与顺铂协同作用下HL60细胞形态变化；MTT法测定不同浓度的顺铂与mDRA6对HL60细胞存活的影响；琼脂糖凝胶电泳检测mDRA6与顺铂联合对HL60细胞DNA片段化的影响。

The parental BHK and the transfectants stably expressing human DR4, DR5, DcR1, or DcR2 were stained with control mouse IgG or murine antihuman DR4, DR5, DcR1, or DcR2 mAbs and analyzed by flow cytometry. Dotted and solid lines correspondent to control and specific stainings, respectiely. Cell surface staining with anti-DR4, anti-DR5 and anti-Fas mAbs.

抗人TRAIL受体的单克隆抗体的特异性：用小鼠IgG或鼠抗人DR4、DR5、DcR1及DcR2单克隆抗体对未转染的BHK细胞以及分别转染了人DR4、DR5、DcR1及DcR2并稳定表达的BHK细胞进行免疫组化染色，然后做流式细胞分析，点线和实线分别对应于对照及特异性染色。

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

The approximate method of discretization may be used to calculate the persistent spin current in mesoscopic ring.

用离散化的近似方法也可以计算介观环中的持续自旋流。

Fluidization ; functional fiber ; aurum ; reaction rate

流态化；功能纤维VACF ；金；反应速率

In the Eogene and Neogene, Qaidam Basin experienced the multiphase tectonic movement; the paleoclimate evolved from warm-humid to arid; the water in the basin was gradually salinized; the depocenter migrated from west to east. The master factors and developmental characters of lithologic hydrocarbon reservoirs have their own particularity. Taking the fine exploration zones of the southwest hydrocarbon-rich depression in Qaidam as the key point, with the analysis of source-rock distribution, sedimentary evolution and sedimentary system distribution, the author proposes that the density difference increases after the fluvial water enters, then clam and bar sand bodies are formed easily due to density flow. Highstand system tract and transgressive system tract below the maximum flooding surface are the main exploration areas of lithologic reservoir.

柴达木盆地古近纪－新近纪经历了多期构造运动，古气候由温湿向干旱演变、湖盆逐渐咸化、沉积中心由西向东迁移的演化过程，地层岩性油气藏的主控因素和发育特征有其特殊规律，以柴达木盆地柴西南富油气凹陷精细勘探区带为重点，通过分析烃源岩展布、沉积演化和主要沉积体系展布，提出咸化湖盆在河流入湖后因密度差较大，易形成密度流等成因的各类滩坝砂体，最大湖泛面之下的高位体系域、湖侵体系域是地层岩性油气藏的主要勘探领域。

CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型；以硫氢化钠作为H2S的供体；应用CCK-8比色法检测细胞存活率；碘化丙啶染色流式细胞技术检测细胞凋亡率；Hoechst33258染色检测细胞凋亡的形态学变化；罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位；2'，7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。