流化

The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察，采用相差显微镜观察其生长情况，透射电镜观察细胞结构，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞，以MTT比色法描绘生长曲线、检测生长状态，组化法检测老化相关β-半乳糖苷酶，流式细胞仪分析细胞周期和增殖指数。3。

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling . Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

The changes of MDA in the plasma, CINC/IL-8 and NO in the serum, MPO in the skeletal muscle were studied at the end of ischemia and 1h, 2h, 4h, 8h, 12h during reperfusion.

分别于缺血4h末，再灌注1、2、4、8、12h，测定各组血浆中的MDA、骨骼肌组织中的MPO，血清嗜中性粒细胞化学趋化因子(CINC／IL-8)，血清一氧化氮，用流式细胞仪及免疫组化法观察各组白细胞上粘附分子CD11b／CD18和骨骼肌血管中ICAM-1的表达情况及各组的组织学改变。

A larger error often occurs in measured results by multi-specimen J-R curve method because of the difficulty in determining the blunting line equation. On the basis of considering the effect of loading rate on plastic-flow stress, a new blunting line equation in dynamic loading has been obtained: J=2NK0.5(subscript εFΔa.

针对多试样J-R阻力曲线法因钝化线的不易确定性常造成测试结果J误差较大的情况，考虑了加载速率对塑性流变应力的影响，给出了一新的动载条件下的钝化线方程：J=2NK0.5

Water Cascade Analysis technology was used to synthesize the water use network for batch processes,which could determine the water pinch point location and the minimum utilities target of the batch process:minimum fresh water use and wastewater discharge.

利用水级联分析法对间歇过程用水网络进行研究，确定过程的用水夹点及最小新鲜水用量和最小废水排放量，提出了间歇用水过程的全局夹点的概念，考虑了用水过程的流率限制，在水级联表格中用流率替代了传统水级联分析法中的传质量，识别过程中每个时间段的局部夹点及公用工程目标，通过全局夹点和局部夹点的比较，指出过程的用水瓶颈，设置储罐打破瓶颈，使用水网络公用工程目标最小化。

Main work of this text:First, in view of the use widespread some type parameter cage control valve, use the three-dimensional modeling software to establishment valve interior flow field model, through CFD preprocessor production computation grid, using CFD software to separate solve, may obtain the regulating valve interior flow field the visible graph, like pressure and velocity vectors chart, contour map and so on.

本文主要工作：一、针对使用广泛的某一类型参数的套筒型调节阀，应用三维建模软件建立阀门内部流场模型，通过CFD前处理器生成计算网格，应用CFD软件进行离散求解，可以得到调节阀内部流场的可视化图形，如压力、速度等矢量图，等值线图等。

Phase-lagged cyclic boundary condition is implemented by a novel method in conjunction with the developed sliding interface technique to accurately simulate the arbitrary pitch ratio rotor/stator interaction without any modifications of the physics of the problem. It's for the first time to compose the computational area into one structured grids including both rotor/stator of centrifugal or axial turbomachinery with arbitrary pitch ratio. This method can eliminate the iteration of boundary conditions on the sliding interface and calculate the unsteady flow within rotor and stator simultaneously.

为了能够在较小的计算区域内不改变叶型而真实地反映出任意栅距比时的内部非定常流场，并避免近似仍采用相位后滞周期性条件，结合本文发展的网格技术，给出了一种新的实施方法，首次将动、静叶片数不等的离心式/轴流式叶轮机械转子、静子计算区域组合在一套结构化计算网格中，动/静叶排间的滑移界面完全按内场处理，避免了每一时层内，滑移界面处边界条件的迭代计算；并保证了每一时层，滑移界面处流动参数的守恒特性。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。