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Keywords : pomp18 gene;clone;expression;Chlamydophila abortus

中文关键词: pomp18基因;克隆;表达;流产嗜性衣原体

Cell resource and animals: Authorized by the Ethics Committee of the hospital, and with the informed consent of gravida, we obtained the fetal brain tissue from 12-week abortus.

细胞来源及动物:经医院伦理委员会授权,孕妇知情同意下留取孕12周流产的人胎儿脑组织。

Key words: Brucella abortus;omp25 gene;cloning;prokaryotic expression

流产布鲁氏菌;omp25基因;克隆;原核表达

For suspected cases, in early pregnancy (8-12 weeks), chorionicvilluscell karyotype analysis was done in those with spontaneous natural abortion; in the midtrimester (13-24 weeks) and the late pregnancy (over 25 weeks), the abortus or newborn blood was collected for chromosome analysis.

对有胎儿异常可能的病例,孕早期(8~12周)自然流产者行绒毛细胞核型分析,孕中期(13~24周)及晚期(25周以上)取流产儿和新生儿血进行染色体分析。

Swine;Chlamydophila abortus; omp1 gene;clone; prokaryotic expression

猪;流产嗜性衣原体;omp1基因;克隆;原核表达

Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200232,ChinaAbstract: Nine samples from swine abortus were detected by RT-PCR with a pair of primers specific to NS1 gene of Japanese encephalitis virus.

采用反转录聚合酶链式反应方法检测猪流产胎儿病料中的乙型脑炎病毒,并对阳性病料做病毒分离与纯化。

There are several distinct pathogens: Brucella abortus, B. canis, B. melitensis, B. suis, whose identifications correlate with their reservoir s, respectively, cattle, dogs, goats and sheep, and pigs.

有若干不同的病原体:流产布鲁氏杆菌、犬布鲁氏杆菌、山羊布鲁氏杆菌、猪布鲁氏杆菌等,其识别与对应的贮藏宿主有关,即分别为牛、犬、山羊和绵羊、猪。

There are several distinct pathogens: Brucella abortus, B. canis, B. melitensis, B. suis, whose identifications correlate with their reservoirs, respectively, cattle , dogs, goats and sheep, and pigs.

有若干不同的病原体:流产布鲁氏杆菌、犬布鲁氏杆菌、山羊布鲁氏杆菌、猪布鲁氏杆菌等,其识别与对应的贮藏宿主有关,即分别为牛、犬、山羊和绵羊、猪。

MATERIALS: The abortus was from healthy healthy pregnant women with HBsAg. The TAT-PDX1 protein was supplied by Tang Dongqi, a professor of University of Florida Health Science Center.

材料:流产胎儿来源于肝功能正常、HBsAg的健康产妇,TAT-PDX1融合蛋白由美国佛罗里达大学医学院唐东启博士惠赠。

There are several distinct pathogen s: Brucella abortus, B. canis, B. melitensis, B. suis, whose identifications correlate with their reservoirs, respectively, cattle, dogs, goats and sheep, and pigs.

有若干不同的病原体:流产布鲁氏杆菌、犬布鲁氏杆菌、山羊布鲁氏杆菌、猪布鲁氏杆菌等,其识别与对应的贮藏宿主有关,即分别为牛、犬、山羊和绵羊、猪。

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The reasons of iron ions content overproof in grade Ⅱ desalting water system,such as variation water quality,contamination of regenerant , operation adjustment of pretreatment system and switching operation of bed were discussed.

对二级脱盐水系统中铁离子含量超标的原因,如来水水质发生波动、再生剂受到污染、预处理系统操作调整、床体运行切换等进行了论述。

You were hired to drum up new business, so go and do it.

公司雇你招徕新业务,你就做你的事好了。

Who is in possession of this?

这是谁的?