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Polyethylene glycol (MW6000 35%)-Ca〓(40mmol/l) system was applied to research on the fusion of nuclei and anucleated protoplasts which were deactivated by ultraviolet rays. The fusion process was verified by -dCTP radioisotope tracing with radioautography.

选择PEG6000促融,40mmol/1Ca〓进行了K氏酵母细胞核与His酵母经紫外线灭活的无核原生质体融合的研究,利用放射性同位素dCTP的示踪结合放射自显影技术,对融合进行检测,证实了融合的确实发生。

That is how it will remain a living, flourishing, and reproducing entity — the very life of God.

那就是它如何会保留一个活的、繁荣的、和繁殖的实体——神的命根子。

Because a rotating joint is essential to a wheel, a wheel made of living cells would twist

因为一个旋转的接头对轮子来说是至关重要的,由活的细胞构成的轮子在第一次转动时便会

A plant ,such as a parasite or saprophyte,that obtains its nourishment from other living or dead organisms.

异养植物一株从其他的活的或死的有机物身上得到养分的植物,如寄生植物或腐生植物

And to the angel of the church in Smyrna write:'The words of the first and the last, who died and came to life.

「你要写信给士每拿教会的使者,说:『那首先的、末後的、死过又活的,说

8To the angel of the church in Smyrna write: These are the words of him who is the First and the Last, who died and came to life again.

8「你要写信给士每拿教会的使者,说:『那首先的、末后的、死过又活的,说

And unto the angel of the church in Smyrna w***te; These things saith the first and the last, which was dead, and is alive

2:8 你要写信给士每拿教会的使者说,那首先的,末后的,死过又活的

As this is an authentic, unvarnished insiders' tour, in a "live" working environment, you'll never know what or whom you may run into!

由于这是一个真实的,无任何装饰的,在一个"活的"工作环境中的内幕参观,您将不可预料会偶然发现或遇到谁。

Thesecond,Leibniz finds intentionality is essential to consciousness,and in hisphilosophy the perception and appetition of the monad have vividintentionality.

其中知觉的意向性使得单子与世界不可分割地联系在一起,成为宇宙的活的镜子,正如海德格尔所指出的,单子是一种&在世界中存在&。

Methods: The primary PAEC had been cultured and passage with porcine autoserum. PAECs were plated in 96-well microculture plates, and incubated with 5%AB normal human serum, 5% heat-inactivated FCS and 5% no heat-inactivated FCS for 6 days respectively, then the survival rates were detected by MTT chromatic test and expression of heme oxygenase 1 (HO-1) was observed by immunohistochemical method after these PAECs had been incubated with 25% NHS for 2 hours.

以猪自体血清原代培养PAEC并传代,接种于96孔板后分别以5%正常人血清、胎牛血清和补体灭活的胎牛血清诱导6d,加入25%正常人血清,以MTT比色法检测细胞的存活率,并以免疫组化的方法观察各组HO-1的表达情况。

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For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降,而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新

Lao Qiu is the Chairman of China Qiuyang Translation Group and the head master of the Confucius School. He has committed himself to the research and promotion of the classics of China.

老秋先生为中国秋阳翻译集团的董事长和孔子商学院的院长,致力于国学的研究和推广。