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The enzyme activity in the hemocytes of infected shrimp was remarkably different from the healthy ones. In the infected shrimp, it could also be observed that granules were expelled from hemocyte in a process of cytolysis. The enzymes were thus activated.

健康虾和患病虾血细胞中酶活性的表现形式不尽相同,在病理情况下,颗粒细胞能以细胞解体的方式,排出细胞内的颗粒,转化成有活性的酶,以此提高血淋巴中这几种酶的活性。

Effects of water stress on the level of reactive oxygen speciesand activities of antioxidant enzymes in the leaves of wheat(Triticum durum Desf.) seedlings were investigated.Water stress resulted in significant increase in the contents of O-.2,MDA and the activities of superoxide dismutase and ascorbate peroxidase in wheat leaves.However,the activities of glutathione reductase,catalase and the content of H2O2 were not changed obviously under water stress.

分析了水分胁迫对小麦幼苗叶片中活性氧水平和抗氧化酶活性的影响,结果发现,水分胁迫能够显著提高超氧阴离子(O-2·)和丙二醛的含量及超氧化物歧化酶和抗坏血酸过氧化物酶的活性,而过氧化氢(H2O2)的含量及过氧化氢酶和谷胱甘肽还原酶的活性变化不明显。

The results showed that the biological activities are closely related to the steric conformation and distribution of electrostatic potential of carboxyl group: The coplanatity between the group of C-3 position and the parent nucleus and the coplanatity between the group of C-3 position and C-4 keto group are very important for biological activities.

结果发现该类药物的抗菌活性与C 3位的空间构象和静电势分布有着紧密的联系。C-3位羧基与C-4位酮基共面性、以及它和母核共面性对抗菌活性十分重要,C-3位两个氧原子周围较强的负静电势也是影响活性的重要因素。

Essential oil was extracted from the petroleum ether-soluble fraction of the leaves showed having antibacterial activity. It was demonstrated that the essential oil has significant antibacterial activity and the activity of the petroleum ether-soluble fraction is attributed to it. The constituents of the essential oil were analysized by GC-MS.10 relatively abundant components were identified on the basis of MS, among which Geranlgeraniol was found in the essential oil of Perilla frutescens leaves for the first time.

对具有抗菌活性的紫苏叶石油醚萃取物进一步精制,从中提取出挥发油,通过抗菌测试证明紫苏叶挥发油具有良好的抗革兰氏阳性菌和革兰氏阴性菌活性,是紫苏叶石油醚萃取物抗菌活性的来源;采用GC-MS对紫苏叶挥发油进行了成分分析,得到10种含量较高的主要化合物,其中的牻牛儿基牻牛儿醇首次在该植物挥发油中被发现。

Became higher when cell was treated by Staurosporine while they are lower when treated by Quinacrine or Verapamil.To identify when the cytoxic signal transducts into the plasma and the time efffects of various inhibitor after cytokines combinds with their receptors.

Staurosporine促进细胞凋亡的过程浙江大学硕士学位论文伴随着Caspases和 Caspase3活性的升g,u Qui删rine和 Veraparnil 在抑制细胞凋亡的过程中Caspases的活性变化不大,而Caspase3的活性则相应降低。

Objective: Extracting the total protein from scoipii tegument, investigating its effect on immune system by transformation of T/B cell. Method: Water-soluble protein was extracted by distilled water method and salting-out method, while keratin(ST2) by deoxi-dization method.

中文摘要:目的:为了证明蝎皮对免疫功能的影响并探讨蝎皮中含有的活性成分,通过提取分离蝎皮中的水溶蛋白质和角蛋白质组分,经过体外药理活性筛选,观察其对细胞或体液免疫系统的影响,以期获得具有生物活性的蛋白质组分。

There was no obvious correlation between relative lipophilicity and activity in the current study. Na2Fl showed obvious synergetic action to other xanthene derivatives; it also show obvious synergetic action as combined lipophilic xanthene chemical and hydrophilic xanthene chemical.

由物化性质和生物活性的测试结果可知:光敏生物活性的测试与单线态氧的测试结果保持了高度的一致性,用单线态氧的产率这个指标来评价对微生物的光敏活性比用亲脂亲水性指标更为有效。

MTT assays indicated that the methanol extract from Hericium erinaceus and Lentinus striguellus have stronger cytotoxity against HeLa cells than other extracts.The methanol extracts from Agrocybe sp had the bioactivity of antibacteria and anti-candida.The methanol extracts from Phellinus vanini and Fistulina hepatica had the bioactivity of inhibition on elastase.

MTT测定表明大部分食药用真菌的甲醇提取物具有不同程度的细胞毒活性,猴头菌与巴西革耳甲醇提取物的作用较强;鳞伞甲醇提取物的抗细菌和抗真菌活性较强;牛舌菌和杨黄菌的甲醇提取物具有抑制弹性蛋白酶活性的作用。

Preliminary antitumor activity in vitro of the new compounds indicated that the compounds have obvious inhibitory effect on human leukaemia cell (HL-60) and human galactophore cancer cell (MDA-MB-468) in the concentration of 10-4 mol/L. This result is a basis for further study on the synthesis and biological activity of this kind of compounds.

对所合成的10个新化合物进行了抗肿瘤体外生物活性试验,初步生物活性实验结果表明,该类化合物在浓度为10-4 mol/L时对人白血病细胞株(HL-60)和人乳腺癌细胞株(MDA-MB-468)有明显的抑制作用,在浓度为10-5 mol/L时没有明显的抑制作用,这一研究结果为进一步研究此类化合物的合成、生物活性的研究奠定了基础。

Based on these results and inferred to related reports from other labratories, it was possible to make some analyses and conclusions or inferrences:(1) CD〓AK with tumoricidal activity were induced and expanded in number througth costimulation of PBMC with anti-CD〓 McAb . and r IL-2 ;(2) CD〓AK induced and expanded in such manner did exibit more potent proliferation·ability and cytotoxicity which maintained for lonser time than those of LAK cells, thus CD〓AK was a new variety of antitumor effector cells worth to be explored;(3) CD〓AK could mediate MHC nonrestricted cytotoxicity and kill tumor target cells through inducing necrosis and apoptosis;(4) Normal mature lymphocytes of PBMC could be induced to proliferate and /or to die from apoptosis when they were costimulated by anti-CD〓McAb and rIL-2. Both proliferation and apoptosis were existing in the same cultivation system sugsesting that the presence of rIL- 2 might provide some accessary signals for apoptosis.

以这些结果为基础并参考其它有关文献可能做出如下分析与结论或推论:(1)用抗CD〓单抗和rIL-2共刺激外周血单个核细胞能诱生扩增出具有杀瘤活性的CD〓AK细胞,(2)与LAK相比,用这种方法诱生扩增的CD〓AK增殖能力强、细胞毒活性强而且维持时间长;CD〓AK是一类值得开发的抗瘤效应细胞;(3)CD〓AK能够介导MHC非限制性细胞毒活性,可以通过诱导靶细胞坏死和/或凋亡杀伤肿瘤细胞;(4)正常外周血单个核细胞中的成熟淋巴细胞在受到抗CD〓单抗和rIL-2共同刺激后既可诱导增殖也可诱导凋亡,两者并存于同一体系,推测rIL-2的存在可能为细胞凋亡提供一些辅助信号。

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