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The effect of calcium chloride on activated sludge was researched in the Activated sludge process.

同时研究了普通活性污泥工艺条件下氯化钙盐度对活性污泥活性的影响。

It is included that 4-5 types of active sites exist in the catalytic system and the active site produced polymer with low molecular weight is easy to be activiated while TEA is used as the cocatalyst. While TIBA, the active site produced polymer with high molecular weight and seldom active site produced polymer with low one is prone to be activiated. MAO tends to activiate the active site produced polymer with middle-level molecular weight. In TEA-TIBA catalytic system, TEA and TIBA activiate the corresponding active site and the weight of every active site gradationally varied with the change of the ratio of the two cocatalysts. And the activity of the active site produced polymer with high molecular weight is proportion to the concentration of TIBA.

发现:TEA作为助催化剂时产生低分子量产物的活性中心易于活化,且体系中具有活性的活性中心有4~5种;TIBA易于活化产生高分子量的活性中心,基本上不能活化产生低分子量产物的活性中心;MAO对产生中等分子量产物的活性中心活化能力较强;在TEA-TIBA混合物体系中,TEA和TIBA各自对活性中心的作用表现为明显的互补现象,各活性中心的相对强度随着两种助催化剂之间的比例改变呈现渐次变化,其中产生高分子量产物的活性中心的相对活性(来源:c5AB3fC论文网www.abclunwen.com)与TIBA的浓度有明显的正相关性。

Methods: DECB was dealed with heat , acid , alkali , roasting , organic solvent extracting , microorganism zymolysis , then the method of microrespiromete was used to detect/measure the respiratory activity respectively.

目的:研究小牛血清去蛋白提取物(deproteinated extract of calf blood,DECB)中生物活性物质的物理化学性质;方法:分别将小牛血清去蛋白提取物进行加热、加酸、加碱、烘烤、有机溶剂萃取、接种微生物发酵等方法处理,然后分别用微量呼吸检压法检测呼吸活性,结果:采用加热、加碱、加酸,接种微生物发酵的方法进行处理,DECB刺激呼吸活性的能力没有明显改变,而采用烘烤的方法处理,结果DECB的活性完全消失;用有机溶剂萃取DECB,可明显降低DECB刺激呼吸活性的能力,而有机相中含有了刺激呼吸活性物质。

Dictamnus dasycarps Turcz. also have insecticidal activity to Ostrinia furnacalis, When the concentration was 10g/ml, correct decreased rate reached 92.86%, When the concentration was 0.675ml/g, the correct decreased rate reached 3.57%; Dictamnus dasycarps Turcz. at the range of testing concentration have lower stomach toxic effects to Ostrinia furnacalis.

通过白鲜皮对玉米螟杀虫活性的初步研究表明:白鲜皮对玉米螟表现出很强的拒食活性,在测定浓度范围内其拒食活性均在80%以上,最高可达97.22%。;白鲜皮提取液对玉米螟也有一定的毒杀活性,当浓度为10g/ml时其校正死亡率可达到92.86%,浓度为0.675ml/g时校正死亡率仅为3.57%;白鲜皮在测定浓度范围内对玉米螟的胃毒活性较低,最高才42.86%。

Coli. The antimicrobial activity of the recombinant fusion protein was evaluated in vitro, which exhibited high antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, and Pasteurella multocida, but low antimicrobial activity against Escherichia coil, and no antimicrobial activity against Salmonell choleraesuis.

对该重组蛋白进行纯化与体外抗菌活性的测定。结果表明,重组鸡AvBD6融合蛋白对多杀性巴氏杆菌、金黄色葡萄球菌、枯草芽胞杆菌有较高抗菌活性,对大肠杆菌的抗菌活性较弱,对猪霍乱沙门氏菌无抗菌活性。

The activity of Carboxymethylcellulase rose and peaked on September 8, and then decreased; the activity of Pectinmethylesterase decreased before September 18 ( early and middle stage of maturity), and then rose to the peak value on September 28, and then decreased dramatically; the activity of Polygalacturonasewas lower before September 18, while its change was same as activity of PE after September 18; cellulose and protopectin of granulated juicy sac were higher than those of non –granulated juicy sac respectively, but activity of Cx, activity of PE and activity of PG of granulated juicy sac were lower than of non-granulated juicy sac respectively.

纤维素酶活性在成熟期间随果实成熟上升,到9月8日出现活性高峰后下降;果胶甲酯酶活性在成熟阶段前中期(9月18日)PE活性呈下降趋势,在9月28日出现PE活性高峰后快速下降,汁胞PG前中期活性较低,9月18日后的变化趋势和PE活性的变化一致。粒化汁胞的纤维素,原果胶含量均高于未粒化汁胞;它的CX活性、PE活性,PG活性低于未粒化汁胞。

The results of the determinating the activity of the CA in vitro showed that the CA of the water—fed individuals were in activing statics after eclosion. Because of the apperence of the Vg in haemolymph and the deposition of the vitellin in oocytes in the unfed females, it was shown that the CA in such individuals were in activiting statics, too. Again because in the water—fed females which did not lack the feeding action the activity of the CA showed dropping tendency gradually still, it could be said that the activiting of the CA was possibly effected by the nutrition but it was never related to the adult feeding action. In another respect, the brain of the female adult had not significant effect on the activiting CA. The oocytes could depositing vitellin in individuals removed brain tissue on the day of eclosion, which showed that the activting of CA was not concerned with brain.

CA活性的体外测定说明饲水个体的CA同样具有活性,不取食个体血淋巴中Vg的存在以及卵母细胞中卵黄的沉积表明,在不取食的个体中CA同样具有活性,所以CA的活化与成虫期的取食动作无关,与成虫是否取食花蜜也无太多的联系,但是CA活性的维持可能受成虫期营养的影响,如果以卵母细胞发育和CA体积大小作为CA活性的指标,则饲水个体CA的活性在后期受到了影响,说明饲水不能替代取食蜂蜜来维持成虫期CA的活性;成虫期脑对CA的活化无明显作用,羽化当日摘除脑的个体,其卵母细胞仍然可以沉积卵黄,说明CA的活化与脑无关,而是受某种不确定的因子的影响;用抗JH物质—早熟素—Ⅱ以10ug/虫的剂量在颈部点滴处理雌虫,对Vg的发生和卵巢的发育具有一定的迟缓作用,其原因有待于进一步的研究。

Radioactivity of 〓Tc-MIBI incepted by A549DDP cells in controlling group, Rh2 group, DDP group and Rh2+DDP group account for 0.94%, 1. 08%, 1. 13% and 2. 0% respectively of the total radioactivity. There was evident difference between the former three groups and Rh2+DDP group P<(0.05 . Radioactivity of 〓Tc-MIBI incepted by A549 cells was 2. 38% of the total radioactivity, obviously higher than that in controlling group (P <O. 05). conclusions: gensenoside Rh2 of no toxicity concentration can reverse resistance of lung adenocarcinoma A549DDP cells to cisplatin.

对照组的A549DDP细胞摄取〓Tc-MIBI的放射性活性与总放射性活性的百分比为0.94%为,Rh2组的A549DDP细胞摄取〓Tc-MIBI的放射性活性与总放射性活性的百分比为1.08%,DDP组的A549DDP细胞摄取〓Tc-MIBI的放射性活性与总放射性活性的百分比为1.13%,这两组分别与对照组比较无显著性差别(P>0.05);Rh2+DDP组的A549DDP细胞摄取。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池,将lipid A包被于非衍生样品池,分别建立以PGN、CpG DNA及lipid A为靶点的技术平台,对114种抗炎中药水提物进行筛选、评价其活性物质含量,并评估出针对上述三种病原分子均具有较高结合活性的中药;(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术,从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分;在体外实验中,测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力;MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响;ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用;在体内实验中,采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌,建立细菌脓毒症小鼠模型,观察活性组分对脓毒症模型小鼠的保护作用。

Results showed that soil catalase activity was stimulated by the copper products and the inhibition of urease and sucrase activities by copper products were also observed,especially declined significantly in the treatment of BDM,the two enzymes activities in high ...

研究表明:铜制剂对土壤过氧化氢酶活性有刺激作用,而对脲酶、蔗糖酶活性均有显著抑制作用,尤以BDM对土壤酶活性的抑制率最强,高浓度处理下则未能检测出2种酶活性。

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