活性核

We transformed RMgBr into R-Ti derivatives with the production of byproduct MgBrO~(i-Pr at the same time, but we didn\'t need romove the Mg salt by centrifugation or chelating reagents, for bis[2-(N,N-dimethylamino)ethyl] ether could chelate with Mg atom and thereby reduced the Louis acidity of Mg salt, so we used R-Ti derivatives produced in situ to alkylate the aldehydes induced by BINOL-Ti complexes..

我们通过利用TiO~(i-Pr_4使格氏试剂转化成烷基钛，同时产生具有亲核加成促进作用的副产物MgBrO~(i-Pr，由于BDMAEE能与MgBrO~(i--Pr络合降低其路易酸活性，所以不需要离心或者借助络合试剂完全除去镁盐，而使用原位生成的烷基钛在BINOL-Ti诱导下对醛进行不对称加成。

These ligands were found to be very effective in construction of quaternary carbon centers by using asymmetric allylic alkylation.

这类配体在前手性亲核试剂的不对称烯丙基化构筑手性季碳中心的反应中，显示了较好的反应活性及对映选择性。

The ligands were found to be effective in palladium-catalyzed asymmetric allylic alkylation to construct chiral quaternary carbon center.

这类配体在前手性亲核试剂的不对称烯丙基化构筑手性季碳中心的反应中，显示了较好的反应活性及对映选择性。

Modification of the side chain radical of β-lactam class antibiotic is a possible effective way,while the mother nuclide maintain the activity, but the different side chain radical alternating the drug tolerance of bacterium.

改造β-内酰胺类抗生素的侧链，不同侧链改变了细菌的耐药性，而其母核仍保持抗菌活性，这可能是克服抗生素使用剂量不断增大的一条有效途径。

The lack of antigen-presenting cell activity of CB MNCs in primary mixed lymphocyte culture did not induce allogeneic T cell anergy.

缺乏抗原提呈细胞活性的脐带血单个核细胞在初级混合淋巴细胞培养下不能诱导同种异体反应T细胞的无反应性。

With an orthogonal experiment design , the telomerase inhibitors [azidothymidine , AZT 300 mg/ and lamivudine 150 mg/, per os , bid ,×2 weeks ] and X2irradiation [ total dose 10 Gy (2 Gy ×5) in 1 week ] were used to treat BALB/ c mice carrying breast cancer MA782 for evaluating the influence on peripheral blood cells , bone marrow nucleated cells and telomerase activity.

为了探讨端粒酶抑制剂联合X 线照射对荷瘤小鼠的骨髓抑制作用，采用正交实验设计，应用端粒酶抑制剂[AZT 300 mg/， lamivudine 150 mg/ ]联合X 线照射(2 Gy ×5次/ 1 周)处理移植性乳腺癌(MA782 )小鼠，观察其对骨髓有核细胞、外周血像及骨髓端粒酶活性的影响。

A process for preparing the recombinant chicken gamma-interferon (rCHIFN-gamma) with high activirus activity includes such steps as cloning ChIFN-gamma gene from eukaryotic plasmid to transfer carrier by dual enzyme servenings to obtain recombinant transfer plasmid pFASTBACI-ChIFN-gamma, taking DH10Bac competent cell, adding IngpFASTBAC1-ChIFN-gamma, transposition, diluting to culture object by SOD culture medium, coating on Luria plate, culturing, choosing white clony, purifying, naming positive plasmid as Bacmid-ChIFN-gamma, extracting its DNA, and transfecting Sf9 cells.

高抗病毒活性重组鸡γ-干扰素的制取方法及用途，涉及一种广谱高效抗病毒基因工程产品的制取方法。将ChIFN-γ基因从真核质粒中经双酶切后克隆到转移载体中，获得重组转移质粒pFASTBAC1-ChIFN-γ；取DH10Bac感受态细胞，加入1ng pFASTBAC1-ChIFN-γ，转座后，用SOC培养基稀释培养物，涂布Luria平板，培养后，挑取白色菌落，Luria平板进行纯化，阳性质粒命名为Bacmid-ChIFN-γ，提取阳性质粒DNA；取上述重组DNA质粒转染Sf9细胞，制得高抗病毒活性重组鸡γ-干扰素。

It is possible to preparing neutralizing MAb which target to active region of Stx2 by synthetical B cell epitope peptides. At present, the crystal structure of the compound of Stx2 and its ligand adenines has been resolved, and the active site of Stx2 is definite, which is the seventy-seventh amino acids, Ganimalon, of Stx2 A1 fragment.

Stx2通过B亚基与受体Gb3结合后，进入细胞，在弗林蛋白酶(一种钙敏感的丝氨酸蛋白酶)作用下A亚基被裂解为A1和A2两个片段，A1片断经内质网进入细胞质暴露毒性活性位点―A1片段发挥N-糖苷酶活性，导致真核细胞蛋白质合成的终止，最终导致细胞的死亡。

The calculation shows that two nitrogen atoms of C10H18N2O4Pt are of nucleophilic activity, meanwhile, the platinum atom of C10H18N2O4Pt is of electrophilic activity.

根据计算可知，分子中N16和N20原子有强的亲核活性，Pt19原子有强的亲电活性，得到了C10H18N2O4Pt 分子的HOMO轨道图和IR谱。

The morphologic changes were observed with microscopy. It was observed that the values of NOS, MPO, MDA of the muscle and lung in LIR group increased significantly and the content of PC decreased obviously compared with those of the normal control.

结果显示，与对照组比较，LIR组骨骼肌和肺组织NOS活性均增强，MDA值、MPO活性增加，W/D值增大，肺PC含量降低；光镜下，肺间质多形核粒细胞聚集和浸润，肺间隔面密度值增加。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。