活性核

Objective: To establish a method for detection of NF-κB activity in human T cells after activation using gelelectrophorestic mobility shift assay and enhanced chemiluminescent technique.

目的：建立凝胶电泳迁移率变动分析化学发光法检测T细胞激活后核因子κB活性的方法。

RESULTS: The activity of heparanase in the mammalian cell lysate detected was much higher than that in the culture medium. The molecular mass of the heparanase protein expressed in the transfected COS7 and CHO cells was about 53×103 by western blot. CONCLUSION: The fulllength heparanase gene is isolated.

结果：分离扩增出1629 bp的人HPA全长cDNA序列并构建了其真核表达载体；在转染后的COS7细胞和筛选出的CHO细胞裂解液中检测到较高的HPA活性和相对分子质量大小约53×103的目的蛋白免疫印迹表达带。

The fulllength heparanase gene is isolated. The functional heparanase is expressed within COS7 and CHO cells and few heparanse is secreted into medium. The positive CHO cells expressing active heparanase is screened, which will help explore its function and screen its antibody and antagonists from a variety of sources.

本研究分离出人HPA编码基因全序列；在真核细胞内成功表达出有活性的人HPA，发现此酶在COS7和CHO细胞中表达在细胞内，很少分泌到细胞外；筛选出其恒定表达细胞株，为进一步研究HPA结构功能、开发其抗体及抑制剂提供了有力的工具。

The fulllength heparanase gene is isolated. The functional heparanase is expressed within COS7 and CHO cells and few heparanse is secreted into medium. The positive CHO cells expressing active heparanase is screened, which will help explore its function and screen its antibody and antagonists from a variety of sources.

本分离出人HPA编码基因全序列；在真核细胞内成功表达出有活性的人HPA，发现此酶在COS7和CHO细胞中表达在细胞内，很少分泌到细胞外；筛选出其恒定表达细胞株，为进一步研究HPA结构功能、开发其抗体及抑制剂提供了有力的工具。

Under the support of NSFC, 3 bioactive nucleoside antibiotics against agricultural pathogenic fungi were purified from fermentation broth of DKL producing strain.

本项目从多抗灵产生菌的培养物中主要分离了三个有抗真菌活性的核苷类化合物(DKL-A；DKL-B， DKL-C)。

METHODS: Oleanolic acid and cholic acid were coupled with active small poptides; the activity of the coupling compounds to induce T lymph cell proliferation was studied.

以齐墩果酸和胆酸为母核，分别与活性小肽偶联，考察此类偶联物对T淋巴细胞增殖的影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。