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Ribozyme is a kind of RNA with self-catalytic activity which was found in 1980s.

核酶是80年代初期发现的具有自催化活性的RNA片段。

AIM: To study the interrelation between the structure and the function of artificial ribozyme M1GS.

目的:研究核酶M1GS其二级结构与体外切割活性之间的关系。

To design HDV ribozyme for human telomerase RNA component, and observe its inhibition for telomerase activity .

设计针对端粒酶的HDV核酶,观察其对端粒酶活性抑制。

The c-erbB-2 oncogene-specific ribozyme has a high activity in vitro. It lays a foundation for the study of the therapeutic use of ribozyme in gene therapy of cancer.

结论c-erbB-2特异性核酶RZ1在体外有良好的切割活性,该实验为进一步研究其在肿瘤基因治疗中的作用奠定了基础。

Methods : M1RNA ribozyme targeting the RNA template of telomerase was designed.

核酶是一种具有催化活性的小分子RNA,能序列特异性切割靶RNA。

However,the freeze-drying process provides a new way to solve this problem. The process was conducted the way a sample is precooled to-30℃,and the shelf temperature during primary drying is set to-30℃,which during secondary drying is set to 15℃.The time required for the whole freeze-drying process is a...

将样品预冻到-35℃后,一次干燥时搁板温度控制在-30℃,二次干燥时搁板温度控制在+15℃,整个冻干过程在25小时的工艺下,通过改变脐带血中冻干保护剂组分及浓度,比较冷冻干燥后有核细胞的恢复率,得出恢复率最高(68.397%)的配方(40%PVP+30%海藻糖+10%甘露醇),并比较了细胞冻干前后的形态,然后将样本在常温下放置三周后进行了P I染色活性检测(89.08%)。

Progresses on reactions of NO, CAN, and hydrazone Reactions of NO with olefines, phenols, amines, arylhydrazines, Schiff bases and nucleosides are firstly reviewed.

本论文主要研究了一氧化氮及硝酸铈铵与简单醛酮腙、α,β-不饱和酮腙的反应,全文共分五个部分: 1、一氧化氮、硝酸铈铵及腙的反应研究首先对具有特殊生物活性的NO与烯、酚、胺、肼、Schiff碱、核酸碱基等有机物的反应做了全面的总结。

Aromatase protein and its mRNA were abundant in the forebrain, midbrain, spinal cord, wheel organ and Hatschek's pit, but not abundant in the hindbrain or early ovary and testis. No aromatase expression was detected in both irregularshaped cells and ciliated mucous cells in the Hatschek's pit or in the mature ovary and testis. Aromatase immunoreactive material was distributed in the cytoplasm with negative nuclei.

芳香化酶蛋白和转录物在前脑、中脑、脊髓、轮器和哈氏窝十分丰富,而后脑、早期卵巢和精巢不够丰富;没有芳香化酶表达的部位是哈氏窝另两种细胞(不规则形细胞和带纤毛粘液细胞)以及成熟卵巢和精巢;芳香化酶免疫活性物质分布在胞质,核为阴性。

Advances in Research on Plants Reactions under Phosphate Starvation;2. Topics discussed in this article includes synthesis and structures of R2M24, the reactions of its metal-metal triple bond with various reagents such as nucleophiles, carbon-carbon mu.

本文对一类重要的ⅥB族金属叁键化合物R_2M_2_4(R为环戊二烯基及类环戊二烯基)近年来的研究成果进行了综述,综述重点是这类化合物的官能团M≡M参键的化学活性,全文包括R_2M_2_4的合成及结构,M≡M叁键与亲核试剂、与碳-碳重键,与氧或与金属羰基物等试剂的反应及其应用。

Topics discussed in this article includes synthesis and structures of R2M24, the reactions of its metal-metal triple bond with various reagents such as nucleophiles, carbon-carbon mu.

本文对一类重要的ⅥB族金属叁键化合物R_2M_2_4(R为环戊二烯基及类环戊二烯基)近年来的研究成果进行了综述,综述重点是这类化合物的官能团M≡M参键的化学活性,全文包括R_2M_2_4的合成及结构,M≡M叁键与亲核试剂、与碳-碳重键,与氧或与金属羰基物等试剂的反应及其应用。

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