活性核

Using Pseudomonas syringae and Pantoea ananas , the dominant ice nucleation active bacteria strains from Yunnan province, as receptors of genetic engineering recombination, and Escherichia coli S17/pZJ25∶∶Tn5 as donator. By transposon Tn5 induced mutants method, the Tn5 was inserted and integrated into the genome of wild type INA + bacteria. INA + mutant as INA - when the linear continuous of INA + gene destroyed.

以云南省冰核细菌的优势种类丁香假单胞菌Pseudomonassyringae和菠萝泛菌Pantoeaananas为重组基因工程菌的受体菌，采用Tn5转座子诱变技术，以大肠杆菌EscherichiacoliS17/pZJ2 5∶∶Tn5作为转座子诱变的供体菌，使Tn5插入并整合到冰核细菌基因组中，使冰核基因的线性连续性被破坏，失去冰核活性。

Western Blot was used to test the expression of NF-κB in nuclear and cytoplasm. The intranuclear expression of NF-κB was confirmed by ELISA assay. The influence on cell apoptosis of gastric carcinoma cell by Paeoniflorin and 5-FU was estimated by flow cytometry.

应用Western Blot方法检测各组胞核NF-κB表达情况，并应用胞核NF-κB活性检测进一步确定胞核内NF-κB的表达情况，流式细胞仪检测联合应用芍药苷及5-氟尿嘧啶(5-FU)对胃癌细胞凋亡的影响？

Open field test showed a low level of locomotion, rearing and grooming, which indicate a disruption of engagement in both a social and nonsocial nature and decreased explorational ability. These findings all fit the behaviors of depression. The increased adrenal weight and increased total number of CRF immunoreactive neurons in PVN of hypothalamus indicated the hyperactivity in HPA axis.

以上指标符合抑郁动物模型的标准；肾上腺重量增加和下丘脑室旁核CRF阳性神经元数目增加提示HPA活性升高；DST显示，预给地塞米松(dexamethasone，DEX)不能减少CUMS处理的大鼠下丘脑室旁核CRF阳性神经元数目，与之对应的是，预给DEX能够显著减少急性应激诱导的大鼠下丘脑室旁核神经元的增加，这提示DST的失抑制。

With brain stereotaxis technique PP2A was inhibited in the nucleus basalis of Meynert and the cytoskeletal protein tau, neurofilament were found hyperphosphorylated and aggregating. The neurite became short and thickening, acetyltransferase aggregated in the body of neuron and the acetylcholine level in the nucleus basalis of Meynert was significantly decreased followed with spatial memory defict of rats.

利用立体定位技术抑制大鼠基底核PP2A活性，使基底核神经元微管相关蛋白tau及神经细丝出现过度磷酸化和聚积，轴突变短增粗，胆碱乙酰转移酶在胞体聚集，而使基底核乙酰胆碱含量降低，大鼠出现空间记忆障碍。

Furthermore, this novel approach would have potential in the research on human disease detection and diagnosis synchronously.

为核酶活性分析、核酶动力学研究提供新型有效的手段，也为核酶基因药物的深入研究提供了新的方法和思路。

The rules of photo bioactivities are: Without illumination, the tested xanthene derivatives showed almost the same level of inherent toxicity to the same organism, which showed the inherent toxicity of xanthene derivatives were primarily dependent on the structure of parent molecule. Upon illumination, the photosensitizers showed obvious phototoxicity to all organisms. The xanthene derivatives showed stronger phototoxicity to Gram-positive bacteria. With the increasing number of halogen substituents, the singlet oxygen yields increased and the phototoxic activity increased too.

呫吨类化合物的生物活性的变化规律为：未经光照的条件下，呫吨类化合物的对同种待测微生物的固有生物活性基本相同，说明固有毒性可能主要决定于化合物分子的母核结构，其卤素取代基团对其影响较小；经实验光源照射下，所测MLC值明显低于未经光照条件下的值，表明呫吨类化合物对三种微生物的光敏活性明显强于对其相应的固有活性；呫吨类化合物对革兰氏阳性菌能发挥较大的光敏活性。

Double fluorescent immunohistochemical staining revealed close apposition of VIP-, PACAP-, and vasopressin-immunoreactive terminals with tyrosine hydroxylase-ir neurons within the dorsomedial arcuate nucleus. We were then of interest to learn from where VIP-, PACAP- and AVP-containing neurons that project to the ARN originate.

经由双重免疫萤光染色法，可以在弓状核的背中区，具酪胺酸羟化酶免疫活性的细胞旁，发现有VIP、PACAP及血管加压素免疫活性的神经末稍的分布及并置，显示确有VIP，PACAP及AVP之神经元投射至弓状核。

Results: At the concentrations of 0.1, 1 and 10 μmol/L, berberine dose-dependently suppressed the formation of TRAP-positive multinucleated cells, the TRAP activity and the osteoclastic bone resorption. The strongest inhibitory effect was exhibited at the concentration of 10 μmol/L, with the inhibiting rate of 60.45%, 42.12% and 72.69% respectively.

结果：小檗碱在0.1~10μmol/L范围内，浓度依赖性地抑制TRAP阳性多核破骨细胞的形成和TRAP活性，减少破骨性骨吸收陷窝的面积；在10μmol/L浓度下，对破骨细胞的抑制作用最强，对TRAP阳性多核破骨细胞的形成和TRAP活性的抑制率分别达到了60.45%和42.12%，骨吸收陷窝面积减少72.69%。

The function of bone resorption by MGC was examined with coculture of bone slice and MGC. The resorption lacunae were examined with a scanning electron microscope. The activity of acid phosphatase in MGC was observed by Gomori stain. Results: MGCs showed multinuclei (more than 20 nuclei), irriegular peripheral cytoplasm with pseudopodial projections, vacuoles were seen in the cytoplasm.

用倒置相差显微镜观察体外培养的多核巨细胞的一般形态及降钙素对它的影响；用骨片与多核巨细胞共同培养法观察多核巨细胞的体外骨吸收功能，用扫描电镜观察骨吸收陷窝，Gomori染色观察多核巨细胞的酸性磷酸酶活性。

The results showed that succinate in the growth medium had the negative function to the ice nucleation activity of Xanthomonas campestris IN-1, which was not consistent to the literature.

结果表明，琥自酸抑制了黄单胞茵生长与冰核活性的表达，甘油、蔗糖、乳糖、谷氨酸钠不同程度地促进了冰核活性和单胞菌的生长。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。