活性核

The susceptibilities of the 3th instar larvae of Spodoptera litura fed on diets treated with nicotine and rutin to AucaNPV, fipronil and spinosad were studied. The activities of CarE and GSTs in different treated cutworms were also tested. The results showed that the nicotine enhanced significantly the susceptibility of Spodoptera litura to insecticides, and the susceptibility enhanced as the nicotine concentration in the diets increased.

以在人工饲料中添加植物次生物质的方法，研究了取食不同含量烟碱、芸香苷的斜纹夜蛾Spodoptera litura 3龄幼虫对银纹夜蛾核多角体病毒、氟虫腈及多杀菌素的敏感性，同时测定了各处理组幼虫体内羧酸酯酶和谷胱甘肽转移酶的活性。

It was typically indirect effect that the following variations induced in the progenies of endosperms implanted by heavy ion beams were discovered. 1 In comparison with control, there were very high differences in biological trait, anti-oxidation enzyme activity, MDA content and protein content, chromosome aberration rate and micro-nucleus rate in M1 generation. 2 Shorter stem variants and purple stem mutants were analyzed by RAPD and AFLP. The analytic results showed that the sequences of their DNA were changed, so those variations were not physiological damage only. And there were differences among M2 generation plants of the same sort of variants or mutants, it was indicated that M2 generation may be in disjunction.

M1代的生物学性状、幼苗的抗氧化酶活性、MDA含量、蛋白质含量和染色体畸变率及微核率等发生了较大的变化。2）对其矮杆变异株和紫色茎杆突变体（M2）做RAPD和AFLP分子鉴定等研究，结果显示了矮杆变异体和紫色茎杆突变体的DNA序列发生了改变，而非仅仅产生生理损伤；同一种变异体或突变体，在M2代单株间也存在着明显的差异，表明它们可能还处于分离状态。

Objective To construct the eucaryotic recombinant plasmid of pYES2/LactoferricinB and express in yeast of S.cerevisiae and preliminary verify the antibacterial activity.

目的构建牛乳铁多肽的真核表达质粒pYES2／Lactoferricin B，实现其在酿酒酵母S.cerevisiae中的表达，初步检测其不同变异的体外抗菌活性。

The eucaryotic cell expression vector modulated by HPSE gene core promoter could be successfully constructed, which could express specifically in tumor cell lines, and its activity should be further enhanced.

成功构建了由HPSE核心启动子驱动的真核细胞表达载体，该载体可在肿瘤细胞特异性表达，但其活性需进一步加强。

Objective To construct the eucaryotic recombinant plasmid of pYES2/LactoferricinB expressing in yeast of S. cerevisiae, of which the expressed protein antibacterial activity was verified in preliminary.

目的 构建牛乳铁多肽的真核表达质粒pYES2/Lactoferricin B，实现其在酿酒酵母S.cerevisiae中的表达，并初步检测其不同变异的体外抗菌活性。

Objective To construct recombination eukaryon expression plasmid for human parathyroid hormone gene, assay PTH expression and biological activity after transfection in vitro and evaluate gene therapy effect on hypoparathyroidsm.

目的构建甲状旁腺激素基因的重组真核表达质粒，评价体外转染后PTH基因的表达与生物学活性，同时观察其对甲状旁腺功能减退动物的基因治疗作用。

The pIRES-EGFP-HCN2 eukaryon expression vector is successfully constructed, with double activity of HCN2 and EGFP. IRES-induced exogenous gene HCN2 can express in human embryonic kidney cells.

实验成功地构建了pIRES-EGFP-HCN2真核共表达载体，具有HCN2、增强型绿色荧光蛋白的双重活性，IRES能够介导外源基因HCN2在人胚胎肾细胞表达。

It was demonstrated that the Vp54 gene promoter has activity in both prokaryotes and eukaryotes.

这说明Vp54基因的启动子在原核和真核生物中均具有启动活性。

Methods Cells were maintained in DMEM medium with 10% fetal bovine serum. Five days before the beginning of experiments, the cells were seeded in phenol red-free DMEM medium containing 5% charcoal dextran-treated FBS. The cells were harvested, and seeded in 6-well culture plates or in 75ml flacks. After NP, BisA and DBP treatments for 72h, the cells were harvested, and mRNA and protein expression of PCNA, bcl-2 and bax were detected by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively.

方法］T47D细胞在含10%胎牛血清的DMEM培养液中进行常规传代培养，实验前将细胞转移至无酚红DMEM（含5%活性碳葡聚糖苷处理过的FBS）中继续培养5d，收集细胞，PBS洗涤后接种於内置盖玻片的6孔板或75ml培养瓶中，用32×10^(-7)mol/L NP、32×10^(-7)mol/L BisA及32×10^(-6)mol/L DBP对细胞分别处理72h，用半定量RT-PCR技术观察这3种化合物对核增殖抗原PCNA、bcl-2及bax mRNA表达的影响，并用免疫组化方法对结果进行验证。

Simultaneously, the activity and coding gene expression of ribulose 1, 5-bisphosphate carboxylase, phosphoenolpyruvate carboxylase, and glycollic oxidase all decrease in two rice accessions under low potassium supply while significant decrease in non-allelopathic rice "Lemont".

水稻光合作用相关酶的分析结果表明，低钾条件下核酮糖－1，5-二磷酸羧化酶、磷酸烯醇式丙酮酸羧化酶和乙醇酸氧化酶的活性及其基因表达强度均降低，但化感水稻&PI312777&的下降幅度显著小于非化感水稻&Lemont&。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。