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Results: Both phosphorothioate-modified oligonuclotides and oligonucleotides complementary with telomerase UNA template inhibited telomerase activity. And application of the tow ODNs together increased the inhibition activity in upfold.

结果:硫代磷酸酯寡核甘酸和RNA模板反义ODNs都可以抑制端粒酶的活性,且可产生累积效应。

The fermentation of ice nucleation-active bacteria (Xanthomonas campestris IN-1 )was studied in this paper.

应用正交设计与均匀设计对黄单胞冰核活性细菌的发酵制备条件进行了初步研究。

Results TNFα-induced ICAM-1 expression in HK-2 cells was increased in both protein and mNRA levels( P .01). NFkB inhibitor N-tosyl phenylalanine chlormethyl ketonecould inhibit these effects of TNFα.

方法用 HK-2 细胞作靶细胞,用细胞酶联免疫吸附法和 Northem 杂交观察 ICAM-1 的蛋白和基因的表达,以电泳迁移率变动法测定转录因子核因子 kB和激活蛋白 1(AP-1)的活性。

Activity of alkaline phosphatase and ability of formation for calcifying nodules were determined.

抽取健康成人骨髓组织,用Percoll分离液分离出骨髓中的单个核细胞,在含体积分数为10%小牛血清的高糖DMEM培养液中,置于37℃、含体积分数为5%的CO2湿化空气孵箱中培养,通过传代培养扩增骨髓基质干细胞,传三代时改用含地塞米松、β-甘油磷酸和维生素C的条件培养基培养,用倒置显微镜、HE染色观察增殖和分化情况,并测定碱性磷酸酶活性和钙结节形成能力。

To explore the existence and dynamic changes of the Golgi body-like structure in the log-phase and encysting trophozoites (6, 12, 18 hours) of Giardia lamblia, these trophozoites were stained with C6-NBD ceramide, a specific marker for Golgi body in eukaryotic cells, and then they were observed with fluorescence microscopy.

目的 探讨类高尔基体结构在蓝氏贾第鞭毛虫滋养体和成囊不同时段的存在情况及其动态变化机理。方法用可特异性标记真核细胞高尔基体的荧光染料C6-NBD神经酰胺,标记有活性的蓝氏贾第鞭毛虫滋养体及成囊6、12、18h虫体,并用荧光显微镜观察。

AIM: To investigate the expression of human β-defensin-2 (hBD-2) mRNA and the changes of NF-κB activity induced by lipopolysaccharide in human primary epitheliums. METHODS: The pulmonary primary epitheliums from human were stimulated with LPS and the expression of hBD-2 mRNA was detected by reverse transcription polymerase chain reaction.

目的:观察脂多糖诱导人气道上皮细胞人β防御素-2(hBD-2)的表达及核转录因子κB活性的变化,探讨NF-κB在LPS诱导人气道上皮细胞hBD-2表达中的作用。

METHODS Twelve piggies were randomly divided into two groups. There were six piggies in each group,normal temperature cardiopulmonary bypass and DHCA.

目的 探讨早期巨噬细胞的激活和核因子kappa B活性在深低温停循环肺损伤中的作用及其可能的作用机制。

Extent of etherbond cleavage becomes more and more intensive with higher alkaline concentration, higher reaction temperature, longer reaction time, stronger activity of nucleophilic reagent, and these substituted aryl ethers will become more and more unstable.

碱浓度越高,反应温度越高,反应时间越长,亲核试剂的活性越强,醚键断裂的程度越大,这类化合物就越不稳定。

It was suggested that one of the radical reasons resulting in microspore abortion of alloplasms was the disorder between nucleus and cytoplasm, which caused unbalance of enzymatic metabolism, decrease of activities of SOD and CAT, and damage of membrane systems led by accumulated active oxygen.

异质系核质关系不协调,酶代谢失调,SOD、CAT活性下降,活性氧积累导致膜系统损伤,可能是异质系花粉败育的根本原因之一。

The core colloids modified with metal nanoparticles possess excellent optical, electric and catalytic properties.

金属粒子修饰的核胶球具有的优异的导电、光学和催化活性。

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