活性核

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡，原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化：细胞核固缩，核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘，并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用，与对照组相比有显著性差异(p＜0.01)，在剂量为0.8-8μmol／L范围内，抑制率随剂量的增加而增加，当剂量超过8μmol／L时，抑制率反而下降；3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性，引起DNA降解损伤，反向调节细胞周期活动，促使细胞从G0期进入G1期，抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核，可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

Objective To seek new activated killer cells, the feasibility of preparing LAK cell from umbilical blood was explored.

目的 寻找新的高效杀伤活性的细胞，探讨脐血单个核细胞作为LAK细胞前体细胞的可行性和意义。

Methods Models of leucocytopenia induced by irradiation were prepared by 60Coγ-ray and by cyclophosphamide injection. The shengbaiyin was administrated orally. The white blood cell counts, reticulocyte count, the number of nucleated bone marrow cells,T cell transformation indices, activity of tunmor necrosis factor and interleukin-6(IL-6) in blood of leukopenia mice on the 5th, 10th, 15st day after chymisty therapeutics were checked.

用60Coγ照射和注射给予环磷酰胺的方法形成骨髓抑制和免疫低下小鼠模型，中药生白饮合剂给予口服，以外周血白细胞、骨髓有核细胞、淋转指数以及细胞肿瘤因子、白细胞介素－6(IL-6)活性为观察指标。

Influence of Plant Growth Regulator on the Germination Percentage and Seedling Growth of Lolium perenne L .;3. Detection and Quantification of the Endophyte in Lolium perenne L .

由于碱木素中胶体分子有带电的核和溶剂化外壳，构成亲水基因，从而具有一定的表面活性，掺入混凝土中能起一定的减水效应。

According to our knowledge, there have been a few reports on this field, but none was related to use meiotic inhibited agents for promoting oocyte developmental competence.

有关这方面的研究报道还不多，尤其在以核成熟抑制剂对卵母细胞产生抑制，并观察其后的胚胎发育活性方面。

Objective: To investigate the telomerase activity of peripheral blood mononuclear cells from patients with essentail hypertension.

目的：探讨高血压病患者外周血单个核细胞的端粒酶活性。

Recently, it has been found that the multinucleate complexes play an important role in organism due to their unique catalysis and biological activity.

近年来，人们发现多核配合物在生物体内具有独特的生物活性和催化作用。

This experiment is sought to study the contribution of different gene in osteogenous differentiation of bone marrow stromal cells and optimize the seed cells of bone tissue engineering.

为了优化骨组织工程种子细胞，探讨不同基因对骨髓基质干细胞成骨活性的影响，我们首先利用RT-PCR方法获得了全长BM P-2，VEGF 165及bFGF基因，克隆入真核表达载体pcDNA 3.0，鉴定正确后，经脂质体介导转入分离培养的大鼠骨髓基质干细胞，G 418筛选出稳定表达株。

Objective:To recombine the plasmid PcDNA3.1-sTNFRⅠ,and detect the expression of recombinant plasmid in pericementum cell,and evaluate the bioactivity of expressed sTNFRⅠ.

目的：构建真核表达质粒PcDNA3.1-sTNFRⅠ，并转染人牙周膜细胞观察是否有目的产物的表达，研究其是否具有生物学活性，为进一步研究其在牙周治疗中的作用提供实验基础。

NIPP-1 is a subunit of the major nuclear protein phos- phatase-1 (PP-1) in mammalian cells and potently inhib- its PP-1 activity in vitro.

尼普- 1是一种蛋白质亚基的主要核磷植酸酶- 1（聚丙烯- 1）在哺乳动物细胞高效促使inhib -它的PP - 1活性的作用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。