活性核

Methods The t-PA mRNA in peripheral blood nucleared cells was detected by RT-PCR and the t-PA activity was determined by chromogenic substrate method.

用逆转录-PCR法检测外周血有核细胞中t-PAmRNA的表达，发色底物法测定血浆t-PA的活性。

Methods:The effect of chrysin on HIV1 infection and replication in vitro were detected by cell fusion of MT2 cells and HIV1ⅢB/H9 cells labeled calceinAM and the inhibition study of syncytia formation and p24 production monitored in culture supernatant of MT2 cells infected by HIV1ⅢB, respectively. Flurescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69 by activated CD4+T in response to PHA.

以CR对CalceinAM标记的慢性感染HIV1ⅢB的H9细胞（H9/HIV1ⅢB）和MT2细胞的融合、对HIV1诱导的MT2细胞形成合胞体及对感染HIV1的MT2细胞产生p24抗原的影响，探讨其抗HIV1活性；以PHA刺激人外周血单个核细胞，利用流式细胞术，检测CD4+T细胞CD69表达的百分率，分析CR对免疫活化的影响。

The growth rate and enzyme activities significantly decreased when dikaryotic hypha ofPleurotus ostreatus were irradiated with γ-rays and subsequently treated with either caffeineor Na2-EDTA in comparison with γ-rays treatment alone. The inhibition effect of treatmentwith either caffeine or Na2-EDTA before irradiation was more obvious than that after irradi-ation. Treatment with either caffeine or Na_2-EDTA could cause biological damages on hyphawhen the concentrations of caffeine and Na_2-EDTA were up to 0.5 and 1.0mg/ml respective-ly.

侧耳双核菌丝经γ射线辐照后用咖啡因或Na＿2－EDTA处理，其生长速度和漆酶及多酚氧化酶活性较单一γ射线辐照明显降低，使辐射损伤加剧；辐照前用咖啡因和Na＿2－EDTA处理，抑制辐射损伤修复的效果优于辐照后处理；咖啡因和Na＿2－EDTA浓度分别达到0.5和1.0mg／mL后，单独处理侧耳菌丝会直接引起生理损伤。

The antifungal activities of these materials were investigated and build foundation to develop low poison plant antifungal products. In experiment, polyphenols were extracted by reflux ectraction method which applyed petroleum ether to degrease and methyl alcohol solvent. Phenolic hydroxyl were examined and inspected by colour-developing reaction and the content of total polyphenols was determined by UV-spectrophotometry. Screening test were performed to the antifungal activities of polyphenol extraction by the tube dilution method to the selected ten pathogenic fungus such as Dothiorella gregaria, Rhizoctonia solani, Phyllosticta pirina, and the minimal inhibitory concentration were measured. The column chromatography and thin layer chromatography were applyed to separate and analyse cortex extracts of Dracaena cochinchinensi Lour.

实验部分采用以石油醚脱脂，甲醇为溶剂的回流提取法来提取植物多酚，用显色反应检识酚羟基；采用紫外分光光度法对多酚粗体物进行总多酚含量的测定；选取了聚生小穴壳菌、立枯丝核菌、梨叶点霉等10株常见农林业致病真菌，采用试管液基稀释法对多酚粗提取物进行了抗真菌活性筛选试验，并测定其最低抑菌浓度；采用柱层析法和薄层层析法对其中抑菌效果较好的龙血竭进行分离分析，根据其中3种化合物的光谱数据UV、IR、~(13C-NMR、1H-NMR和MS鉴定其结构。

Methods To use Ce3+ or Pb2+ as capture agents to detect carbonic anhydrase, Na+-K+-ATPase, Mg2+-ATPase, 5' –nucleotidase and NADPH oxidase activities on endosome membrane in epithelial cells of renal proximal tubules、hepatic cell of rat and human leukocytes. with cytochemical method.

用细胞化学方法，以Ce3+或Pb2+作捕捉剂，分别检测碳酸酐酶，Mg2+-ATP酶，Na+- K+-ATP酶，5'-核等酸酶，NADPH oxidase在大鼠肾近曲小管上皮细胞、肝细胞以及人体嗜中性白细胞内吞体膜上的酶活性。

And it is involved in the energy metabolism, cell proliferation, hemogenesis and vessel formation and remodeling.

HIF-1α通过调节多种基因的表达来调节细胞对低氧的适应性反应，是迄今为止发现的唯一的一个在缺氧状态下发挥特异性活性的调节分子，被认为是调节缺氧相关基因表达的关键分子，它同激活蛋白(activated protein 1， AP-1)，核转录因子(nuclear factorκB， NF-κB)和p53等协同作用调节机体对缺氧的反应，其功能涉及能量代谢、细胞增殖、造血作用、血管形成、重塑与收缩等诸多方面。

Aureonucleomycin is an antibiotic ,which was developed by Shanghai Pesticide Research Institute. The modification of Aureonucleomycin and the herbicidal activity of the modification products were reported in this paper

金核霉素是上海市农药研究所自行研制的农用抗生素，本文介绍了一些对其进行结构改造的工作，并对改造后得到的产品作了除草活性的介绍

Novel phosphazene cyclomatrix network polymers were synthesized via nucleophilic displacement of activated nitro groups of tri (4-nitrophenoxy)tri cyclotriphosphazene and hexa cyclotriphosphazene with the hydroxyls of bisphenol A.

以三对硝基苯氧基三苯氧基环三膦腈和六对硝基苯氧基环三膦腈为主要单体，采用活性硝基亲核取代的方法，合成了一类新的环簇膦腈聚合物。

Novel phosphazene cyclomatrix network polymers were synthesized via nucleophilic displacement of activated nitro groups of tri (4-nitrophenoxy) tri cyclotriphosphazene and hexa (p-nitrophenoxy) cyclotriphosphazene with the hydroxyls of bisphenol A.

以三对硝基苯氧基三苯氧基环三膦腈和六对硝基苯氧基环三膦腈为主要单体，采用活性硝基亲核取代的方法，合成了一类新的环簇膦腈聚合物。

Design of strength calibration application program for pump shaft parts;2. The calibration of parameters is a key step in the application of IWA Activated Sludge Models.

对国际水协的活性污泥法动力学模型在应用中遇到的参数校核问题进行研究，总结了目前常用的三种方法：专家法、灵敏度分析法和过程工程法。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。