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The overall structure of ACF and ACF-2 are very similar, and it is also similar to that of habu Ⅸ/Ⅹ-bp and that of habu Ⅸ-bp come from Trimeresurus flavoviridis.

由于中央loop的结构交换,ACF-1和ACF-2的凝集素活力丧失,但却产生了新的活力,即结合凝血因子factor Ⅹ的活力

A diverse range of effects:on protein solubility and secretion was observed,and these effects were highly dependent on the particular chaperone.In the host strain over-expressing chaperone SecB harboroing pSecB,the amount of total periplasmic proteins was increased by about 71%and the activities of alkaline phosphatseand GL-7-ACA acylase(GL7A)were increased by about 54%and l.5-fold respectively.In the host strain over-expressing chaperone GroEL bearing pGroEL,the amount of total periplasmic proteins was increased by 52%,and the activity of penicillin G acylasewas in creased by about 76%;about 90%of Hexameric Calcitonin(Cal6)inclusion body and l5%of MS2interleukin3(MS2-HIL3)inclusion body became soluble respectively.All the data were compared with the parental strains un-bearing plasmdis pSecB or pGroEL.

在过量表达SecB的宿主菌中,周质空间分泌蛋白总量较对照组提高了约71%,GL-7-ACA酰化酶在周质空间酶的活力较对照组提高了约1.5倍,碱性磷酸酯酶在周质空间酶的活力较对照组提高了约54%;在过量表达GroEL的宿主菌中,周质分泌蛋白总量较对照组提高了约52%,青霉素G酰化在周质空间酶的活力较对照组提高了约76%,鲑鱼降钙素六聚体的可落性组分的比例由原来的45%增加到约90%,而MS2-人白介素-3融合蛋白的包涵体有约15%转变为可溶性组份。

Trends of overground physiological and biochemical indexs are almost the same—weak soil Naphthalin pollution stress stimulate them,strong pollution restrain stress them. In the first two weeks of seeding stage weak soil Naphthalin pollution stimulate root activity,but soil Naphthalin pollution stress restrain root activity from the second week till jointing stage, In corn grouting stage soil Naphthalin pollution stress stimulate root activity.

部分的生理生化指标都有促进作用,而高浓度的土壤萘污染胁迫对玉米地上部分的生理生化指标有抑制作用;苗期的前两周低浓度土壤萘污染胁迫对玉米的根系活力有促进作用,苗期第三周到拔节期土壤萘污染胁迫抑制玉米的根系活力,而到了用营养生长到以生殖生长为中心的灌浆期土壤萘污染对玉米的根系活力有促进作用。

Methods Spectrophotometry was used to study the activity of PLAP in the presence and absence of L Leu. PLAP relative activity was calculated according to the absorbance variation.

方法应用分光光度法分别测定有L-Leu和无L-Leu存在下的酶活力,根据其活力变化求出酶的相对活力;用双倒数作图法,确定L-Leu对PLAP的抑制类型并求出其抑制常数;同时也观察了pH对抑制作用的影响。

The characteristics of the thermostableα-amylase were also discussed. The optimal temperature and pH for the enzyme were determined as 95℃and 10.0 respectively.

通过对酶性质的研究可得知:酶的最适反应温度为95℃,最适酶作用pH10.0,适量浓度Ca2+在60℃~80℃的范围对酶活力有激活作用,在80℃以上对酶活力有抑制作用,过高浓度的Ca2+对酶活力有抑制作用。

Soaking seed of rice and wheat with zymolytic liquid of Trichoderma harziaiarum, Trichoderma DWC and Trichoderma viride, comparing with germinate ratio, germinate force, root length, bud length and ratio of root to bud, the results show that 50 times dilute zymolytic liquid of Trichoderma harziaiarum has a relatively notable promote role for germinate of seed .The effect of Trichoderma viride to germinate of seed is not evidence. Trichoderma DWC for seed sprout has certain inhibition.

据此,采用哈茨木霉作为出发菌株,通过离子束诱变,从大菌落株中筛选出促进生长的高产突变株——h-13菌株,通过对用该菌株发酵液处理后的水稻及小麦的种子的各项生理生化指标的测定,发现该菌株比其它菌株更能促进水稻和小麦的活力和发芽率,三天时小麦的总根长为3.03cm,比对照的1.42cm的两倍还多,并且能够明显提高种子发芽后的淀粉酶和蛋白酶的活力,其活力都是对照的两倍左右。

The highest agglutinating activities were observed at 24 h post-injection.

溶血活力在注射疫苗后有所增强,注射后6 h时1.08×106、1.08×107cell/ml组的活力达到最高,而1.08×108cell/ml组12 h时活力达到峰值。

And then, etiolated seedlings were stressed under the 25℃、0℃、-10℃ and -20℃, which resulted in decrease in POD activity, and that under -10℃ and -20℃ were the weakest, but there were no markedly difference between them. And the POD activity of Jingdong 8 and SCREEM were stronger than others at any temperature.

4种温度条件下25℃时的酶活力较强,0℃次之,-10℃和-20℃较低,且-10℃和-20℃下的酶活力基本一样;在各温度条件下,冬性品种京冬8号和SCREEM均表现出较强的酶活力,但没有特殊的同工酶谱带。

After treatment with chlorfluazuron to the larvae, the activities of POD in the larvae of three instars were all higher than the control, while the activities of POD in the second and third instar were only 65.0% and 99.2% of those in the control.

抑太保处理后,过氧化物酶活力除2龄幼虫在处理后5 h下降,仅为对照的65.0%,3龄幼虫在处理后1 h为对照的99.2%外,3个虫龄幼虫的POD活力均高于对照。H2O2由0增加至0.1 mL,POD活力呈线性关系上升。

In Chapter Two, some methods such as electron microscope, DNA ending label and MTT measurement were used to studied the changes of apoptosis, necroses and cell vigour of leukaemia K562 cell trunk and HL-60 cell trunk irradiated with millimeter wave. Different effects of millimeter wave to leukaemia cell trunks and human marrow cells were observed clearly. Human marrow cells were not so sensitive to millimeter wave radiation as leukaemia cells.

第二章,利用电镜技术、DNA末端标记、MTT活力测定等方法,研究了毫米波对红白血病K562细胞株、人类急性早幼粒白血病细胞株HL-60及正常人骨髓单核细胞的细胞超微结构、细胞凋亡、细胞活力的影响,结果发现毫米波辐射对正常人骨髓细胞及白血病细胞的影响明显不同,正常人骨髓细胞所受影响较小,特别是功率密度小的毫米波对其无影响;而白血病细胞受辐射后发生显著的凋亡或坏死,细胞活力显著下降,该效应具有明显的功率窗和时间窗。

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