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Results: Compound 909 could remarkably improve the learning-memory abilities of dementia rats. It could inhibit the activity of AChE, increase the level ss of ACh, enhance the activity of SOD, depress the levels of MDA and NO. reduce the expression of APP, and decrease blood viscosity, plasma viscosity and indexs of erythro cyte aggregation. We also made it clear that it could inhibit the apoptosis and upregulate the expression of bcl-2 gene in the PC 12 cells injured by QA .

结果:Co-909能够显著提高痴呆模型大鼠的学习记忆成绩:抑制AChE活力,增加ACh含量,提高SOD活力,降低MDA和NO含量,减少APP的过量表达;降低全血粘度,血浆粘度及红细胞聚集指数;能够抑制喹啉酸所致PC12细胞的凋亡,上调Ecl-2基因表达。

Methods Treated with different cryoprotectors,fat granule was stored at -16℃,-80℃(ultra-low-temperature refrigerator)and -196℃respectively.

在-16℃、-80℃及-196℃条件下,加入不同的低温保护剂,冷冻脂肪组织,24h后测定肌酸激酶活力,并与贮存前酶活力做比较。

The AKP activities of experimental groups in serum and haemocytes were much higher than control grou...

血清和血细胞实验组的碱性磷酸酶活力仅在1h时,溶菌酶活力分别在1h和15 h时显著高于对照组。

In vivo, composition E markedly protected mice from lethal challenge by heat-killed E. coli and heat-killed S. aureus. Conclusions:(1) Targeting on PGN, CpG DNA and lipid A, we successfully established the platform to screen anti-inflammatory traditional Chinese medicine using biosensor technology which is highly effective, objective and feasible.

该组分经MTT法测定对小鼠RAW264.7细胞活力无影响(≤400μg/ml),经CCK-8法测定也对小鼠RAW264.7细胞活力无影响(≤800μg/ml);对PGN、CpG DNA及LPS刺激小鼠RAW264.7细胞分泌TNF-α有显著的抑制作用,并呈现良好的剂量-效应关系;对致死剂量的热灭活大肠杆菌和热灭活金黄色葡萄球菌攻击小鼠有显著的保护作用。

They are more chemically reactive and more bioactive," Illuminato says, because of their size, which allows them to easily penetrate organs and cells."

它们更具有化学活力和生物活力,&Illuminato告诫道,因为它们尺度极为微小,因而穿透器官和细胞都极为容易。&

Result]High concentration of leachates had significant inhibitory effects,but low concentration of leachates had no obvious effects in the initial stage and later stage after treatment.Low concentration of alcohol leaching liquor might had stimulating effects on SOD active.Water leaching liquor had no obvious effects,but higher concentration of alcohol leaching liquor had significant inhibitory effects on root vigor.

结果]在油松针叶水提液和醇提液处理后的初期(10 d)和后期(30 d)的抑制作用仅在高浓度处理;低浓度醇提液对草坪草'抢手股'SOD活性可能有促进作用;水提液对根系活力没有明显的影响,较高浓度的醇提液对根系活力有明显的抑制作用。

Wheares under buffer pH5.2, there is no strong static electricity and instead being a longer wavelength movement; SDS\'s participation just restricts the relative movement of heme\'s ethylene; a new fluorescent peak forms and the reduction of enzymatic activity is due to the packing of micella\'s formation.

强变性的阴离子表活剂SDS与带净正电荷和弱负电荷的SBP(处于pH2.6和4.2缓冲液中)有强烈的静电力作用,酶活力丧失,二级结构变化,荧光强度增加,特征Soret发生蓝移,卟啉环的π-π*共轭的分裂能增大;而当SBP带净负电荷(pH5.2)时,没有静电吸引力,Soret带发生红移;SDS的引入只是限制了血红素上乙烯取代基的运动,使其与卟啉环共轭:极性环境的变化导致产生新的荧光峰;酶活力因胶团包裹作用而有下降。

Purging influences the Kefir grains greatly, the vril of the Kefir grains can only be the vril we need under the continuative culture for 16 generations (pH3.75) even though we purge it every other generation. Under the same technological condition, we draw such a conclusion, so it is of more practical significance for us to use the method of non-purging continuative subculture.

清洗对开菲尔粒的影响极大,既使采用隔代清洗开菲尔粒的活力也需连续培养十六代才能达到所需活力(pH值3.75),在工艺条件完全相同的情况下得到这一结论,显然采用不清洗连续传代更具有实际意义。

Results The activity, recovery rate of activity and half-life of immobilized SOD were 333 U/g, 86.32% and 43.8 d respectively. After storage at room temperature for 5 d, the relative activity of SOD was still more than 80%. The optimal reaction temperature of immobilized SOD was 45℃, and the activity retentions after being used for the first and second times were 70.12% and 51.72% respectively. Both the optimal pH values for SOD before and after immobilization were 6, while the Km values were 0.16 and 0.18 mmol/L respectively.

结果 固定化超氧化物歧化酶活力为333U/g,酶活回收率为86.32%,半衰期为43.8d;固定化酶室温保存5d后,相对酶活力仍保持在80%以上,最适反应温度为45℃,使用一次后回收率为70.12%,重复使用两次后回收率为51.72%;固定化酶与溶液酶在pH6时活性最强,Km分别为0.18mmol/L和0.16mmol/L。

In conclusion, the Pine seed oil has an antisenile effect in rats, the effects were due to a reduction in MDA value and a significantly increasing in SOD activity.

测定指标为大鼠血清中超氧化物歧化酶的活力及过氧化脂质降解产物的含量,研究结果表明:红松仁油能显著地增强大鼠血清中SOD的活力,降低血清中MDA的含量。

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