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The recombinant enzyme hydrolyzed p-nitrophenyl esters but not tributyrin and tricaprylin, and exhibited the highest specific activity (1.1 μmol min-1 mg-1) to p-nitrophenyl caprylate.

SsoPEst不水解三酰甘油酯,可以水解对硝基苯酚酯,对对硝基苯酚辛酯水解活力最高,为1.1 μmol min-1 mg-1,催化对硝基苯酚酯水解的最适pH为5.5,最适温度为80 °C,酶溶液置于80 °C 热处理1小时仍有60 %活力

CySeCD exhibits better GPX activity than 6-AnSeCD in the reduction of H2O2 and cumenyl hydroperoxide by glutathione, respectively.

采用双酶偶联法测定GPX的活力结果显示,6A,6A'-二环己胺-6B,6B'-二硒桥联-β-环糊精(6-CySeCD)催化谷胱甘肽还原H2O2和枯烯H2O2的活力均比6-AnSeCD的高。

After treated by ultraviolet and EMS, a mutant M2-4-4 was produced from Cytophaga MCA-2, with a higher enzyme activity of 66U/ml.

经过多次富集和反复筛选,从海洋环境中筛选到69株具有卡拉胶降解活性的菌株,以产酶活力高、生物学性状优良的MCA-2菌株为出发菌株,进行复合诱变和自然选育,获得了一株酶活显著提高的突变株M2-4-4,发酵液产酶活力达到66U/ml。

Result: At most up to 500 mg·L-1, tetrahydropalmatine showed no sinificant effect on the cell viability, while dehydrocorydaline, berberine and palmatine significantly decreased the cell viability, exceeding 6.3, 0.6 and 6.3 mg·L-1, respectively( P .05 or P .01). Tetrahydropalmatine, dehydrocorydaline, berberine and palmatine significantly inhibited LDH leakage induced by anoxia-reoxia injury, at concentrations of 50-100, 1.25-5, 4 and 30 mg·L-1, respectively( P .05 or P .01). None of the four compounds showed significant effect on the hydrogen peroxide injury.

结果:多至500 mg·L-1的延胡索乙素对细胞活力无任何影响,脱氢紫堇碱、黄连素、巴马汀分别在质量浓度大于6.3, 0.6,6.3 mg·L-1时,显著降低细胞活力( P 。05, P 。01);延胡索乙素、脱氢紫堇碱、黄连素和巴马汀分别在质量浓度为50~100, 1.25~5, 4,30 mg·L-1时,显著抑制缺氧复氧导致的LDH漏出( P 。05, P 。01);4种单体对过氧化氢损伤无显著的保护作用。

Result: At most up to 500 mg·L-1, tetrahydropalmatine showed no sinificant effect on the cell viability; while dehydrocorydaline, berberine and palmatine significantly decreased the cell viability, exceeding 6.3, 0.6 and 6.3 mg·L-1, respectively( P .05 or 0.01). Tetrahydropalmatine, dehydrocorydaline, berberine and palmatine significantly inhibited LDH leakage induced by anoxia-reoxia injury, at concentrations of 50-100, 1.25-5, 4 and 30 mg·L-1, respectively( P .05 or 0.01). None of the four compounds showed significant effect on the hydrogen peroxide injury.

结果:多至500 mg·L-1的延胡索乙素对细胞活力无任何影响,脱氢紫堇碱、黄连素、巴马汀分别在质量浓度大于6.3, 0.6,6.3 mg·L-1时,显著降低细胞活力( P 。05或0.01);延胡索乙素、脱氢紫堇碱、黄连素和巴马汀分别在质量浓度为50~100, 1.25~5, 4,30 mg·L-1时,显著抑制缺氧复氧导致的LDH漏出( P 。05或0.01);4种单体对过氧化氢损伤无显著的作用。

METHODS Diastatic activity,protease activity,intestine impulse rate were determined and the data were analysed using one-factor analysis of variance and rank sum test,to evaluate the prescription of Banxiaqu.

方法以半夏曲的淀粉酶活力、蛋白酶活力和小肠推动率为评价指标,用单因素方差和秩和检验进行分析,研究半夏曲的处方。

In hypoxia/reoxygenation model(Na2S2O4), cell survival was enhanced and LDH in culture medium was reduced(P.05, P.01) after treated with 60, 30μgmL^(-1) Sanguis Draconis flavones. Enhanced cell survival and reduced LDH in culture medium(P.05, P.01) also observed in cytotoxicity model induced by H2O2 after incubated with 60, 30, 15μgmL^(-1) Sanguis Draconis flavones.

在缺氧/复氧(Na2S2O4)模型中,60、30μgmL^(-1)龙血竭总黄酮能显著降低培养液中的LDH浓度,增强细胞活力(P.05, P.01)。60、30、15μgmL^(-1)龙血竭总黄酮能抑制H2O2引起的损伤,降低培养液中LDH的浓度,增强受损细胞活力(P.05, P.01)。

Aqueous extract of cotton has allelopathic effect on Raphanus sativus, Brassica campestris, Lactuca indica, Brassica campestris, Echinochloa crusgall, Setaria viridis and Abutilon theophrasti and its inhibition effect gradually strengthens with increasing concentration. Aqueous extracts from stems, leaves and roots of cotton inhibit or accelerate seed germination and seedling growth, and activity of enzyme of seedling root of cotton. While aqueous extracts from stems and leaves of cotton are 0.02 gmL^(-1), root length, shoot length, lateral root number and dry weight of cotton respectively increase by 10.3%, 3.1%, 22.9% and 2.4% over the control. The above mentioned indexes are lower than the control by 34.4%, 39.2%, 40.6% and 19.0% respectively as the aqueous extracts are 0.1 gmL^(-1). At the same time, root activity and SOD activity decrease by 38.2% and 36.7%, while POD activity and MDA content increase by 52.3% and 36.5%. Growth indexes of plant receptors increase at low concentrations but decrease at high concentrations of aqueous extracts. The allelopathic effect of aqueous extract of stems and leaves is stronger than that of root.

结果表明:棉花植株水浸提液对萝卜、白菜、莴苣、油菜、稗草、狗尾草、苘麻均具有化感效应,随着作用浓度增加,抑制效应逐渐加强;棉花茎叶和根系水浸提液对棉花种子萌发、幼苗生长、幼苗根系活力、根系酶活性有抑制或促进作用,当茎叶浸提液浓度为0.02 gmL^(-1)时,棉花根长、苗高、侧根数、干重分别比对照增加10.3%、3.1%、22.9%和2.4%,当浓度为0.1 gmL^(-1)时,上述指标与对照相比分别降低34.4%、39.2%、40.6%和19.0%,而根系活力、SOD活性比对照降低38.2%、36.7%,POD活性、MDA含量较对照增加52.3%和36.5%,不同浓度棉花植株水浸提液对棉花生长呈现&低促高抑&的规律,且茎叶浸提液作用效应大于根系浸提液。

The SOD activity increased in the four types of embryonated eggs before the 14th day during the incubation.

鸡胚蛋孵化期间SOD活力0~14d处于增加趋势,14~18d活力下降,在14d达到最高峰; 7。

In this paper,one superior Aspergillus niger, which could secrete saccharifying enzym with high activity,was selected from Hexi savory vinegar material by useing the transparents ring as elementary index and saccharifying enzymic activity as secondly index.

采用透明圈法进行初筛,以糖化酶活力为复选指标,从接霉制曲后的河溪香醋原料醅中筛选到1株产糖化酶活力高的优势黑曲霉。

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