活

According to recent researches, intracellular signalling pathway systems involved in appressorium differentiation is related with cAMP pathway and MAP pathway. In this research, 21 bacteria strains and Chaetomium cupreum strains were assayed for their antagonism to appressorium formation in M.grisea.

随着稻瘟病菌一系列致病基因的克隆及功能明确，附着胞形成的信号传导途径包括：cAMP途径和MAP途径，这些途径中基因的失活都影响附着胞的形成。

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

That is conveniently beyond the life expectancy of most of the leaders at L'Aquila.

很显然，峰会上的很多领导人活不到那一天。

A Living Fossil Fish: The arapaima is a true prehistoric river monster, with the fossil record showing that it has roamed the waters of the Amazon River since the Jurassic age.

活着的化石：巨骨舌鱼是史前生物，化石研究表明其早在侏罗纪时期就出现了。

And it is more than an interest in arboriculture that keeps him there for hours pottering about with a jar of white clay,which he uses to cover the graft and keep the air from the moist joint .

是这种超乎一般的兴趣爱好驱使他对着那些零碎的活干上几个小时，用一罐白色的粘土糊住新嫁接的还带微湿的枝丫。

Arius∶「That man is going to bring the ARCANA shortly, you will live to see it.

那男人很快便会带著ARCANA来此，你会活著见证此事的。

When we leave this jurisprudence at the epoch of its final reconstruction by Justinian, few traces of archaism can be discovered in any part of it except in the single article of the extensive powers still reserved to the living Parent.

当这个法律学在查斯丁尼安时代经过了最后一次改编后，除了为活着的"家父"仍旧保有广泛权力的唯一条款以外，其中已很难找到古代制度的迹象。

A day, have the time of breakfast, wealthy person archly said right after whole treasure ate two plain wheat roll:The "you see, now live very tight, not equal to you take two plain wheat roll to eat to to ground walk, arrive ground, the plain wheat roll also finished eat, don't hold up stem to live."

一天，吃早饭的时候，全宝刚吃了两个馒头，财主就狡猾地说：「你看，现在活很紧，不如你拿两个馒头吃着往地里走，到地了，馒头也吃完了，不耽误干活。

I think I'll leave you here, Arcturus, among the ashes of your precious dominion.

我要你活着看见我操控整个世界！

He again came to the body and groped arduously with his hands beneath her body. He felt around for a while, shouting,"There's somebody, a baby, he is still alive"

他又来到她的尸体前，费力地把手伸进女人的身子底下摸索，摸了几下，他高声喊"有人，有个孩子，还活着"。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷