活

The genetic inactivation of eggs is the first key stage of androgenesis in fish. In order to observe the effect of UV irradiation for the inactivation of female nucleus of zebrafish the eggs were irradiated by UV with solution-free, pure water, Ringer's solution and Cortland solution. The results showed that abnormal fry were obtained in all groups and haploids were validated. But irradiation with solution was confirmed it could not inactive fully female nucleus and the fertilization rate of the eggs irradiated with pure water was very low.

雌核灭活是雄核发育的第一个关键步骤，为研究紫外线对斑马鱼卵的雌核灭活效果，将斑马鱼的卵采用干法或置于纯水、Ringer氏溶液和Cortland鱼用生理盐水进行紫外线照射，结果表明，各种方法中均得到畸形鱼苗，并经染色体检查大多为单倍体；四种方法中在Cortland鱼用生理盐水中灭活效果最好，120 mJ/cm2紫外线可以使雌核完全灭活；干法处理不能达到雌核完全灭活的目的，在纯水中灭活后的卵子受精率较低。

Faith Evans My heart belongs to you So what could I do To make you feel I'm down with You see me hangin around But you don't know how you make me feel for you, and Each and every day, I try to make some sense of this What you mean to me, I know it could be serious Each and every nite, I dream about just holding you Loving you like this, what is a girl supposed to do I love you I want you You're the one that I live for And I can't take it any more I love you I need you What can I do to make you see You're the only one for me First time I saw your face My heart just erased All the guys I knew before You walked into my life I was the type to never want for nothing Each and every day, I try to make some sense of this What you mean to me, I know it could be serious Each and every nite, I dream about just holding you Loving you like this, what is a girl supposed to do I love you I want you You're the one that I live for And I can't take it any more I love you I need you What can I do to make you see You're the only one for me I love you, I want you, I need you in my life Can't you see what you mean to me Baby come hold me tight I miss you, wanna kiss you Everytime I see your face Baby I'll be waiting for you Each and everyday I love you I want you You're the one that I live for And I can't take it any more I love you I need you What can I do to make you see You're the only one for me You're the only one for me And I know it's meant to be What can I do to make you see My love will carry on So listen to my heart and know You'll find out where my love will go The future lies between us boy Oh babe I love you so I love you I want you You're the one that I live for And I can't take it any more I love you I need you What can I do to make you see You're the only one for me I love you I want you You're the one that I live for And I can't take it any more I love you I need you What can I do to make you see You're the only one for me

我的心属于你如此我可以做什么使你感觉我是下来由于你在附近见到我 hangin 但是你不知道你如何使我同情你，和每个和每个日子，我试着制造一些感觉这你对我意谓的，我知道它可能是严重的每个和每 nite，我梦到仅仅捉住你亲爱的你喜欢这，女孩应该做什么我爱你我想要你你是那一个我活的为而且我再不能轮流它我爱你我需要你我能做使你见到什么你是我的唯一一个第一时间我见到你的脸我的心刚刚抹掉所有的家伙我以前知道你走进入我的生活我是类型从不免费想要每个和每个日子，我试着制造一些感觉这你对我意谓的，我知道它可能是严重的每个和每 nite，我梦到仅仅捉住你亲爱的你喜欢这，女孩应该做什么我爱你我想要你你是那一个我活的为而且我再不能轮流它我爱你我需要你我能做使你见到什么你是我的唯一一个我爱你，我想要你，我在我的生活方面需要你你不能理解你对我意谓的吗被受到的影响宝贝紧紧地捉住我我想念你，想要吻你 Everytime 我见到你的脸我将等候你的宝贝每个和每天的我爱你我想要你你是那一个我活的为而且我再不能轮流它我爱你我需要你我能做使你见到什么你是我的唯一一个你是我的唯一一个而且我知道资讯科技必须当我能做使你见到什么我的爱将会继续如此对我的心听而且知道你将会发现我的爱将会哪里去在我们之间的将来谎言男孩哦小孩我爱你如此我爱你我想要你你是那一个我活的为而且我再不能轮流它我爱你我需要你我能做使你见到什么你是我的唯一一个我爱你我想要你你是那一个我活的为而且我再不能轮流它我爱你我需要你我能做使你见到什么你是我的唯一一个

Of Eight was a cow town, one of活泉will open up after a park, and construction of the GuoShouJing at Memorial Park.

达活泉曾是牛城八景之一，后将达活泉开辟一公园，并在园内建设了郭守敬纪念馆。

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数，结果表明：①酶-底物二元、三元复合物的失活速度明显慢于游离酶，说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用；②在脲作用下，酶的失活和构象变化均为单指数项过程，而且酶的活力丧失和三级结构变化是同时发生的，说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程；③在盐酸胍作用下，酶的构象变化为两相过程，而失活则是单指数项过程，酶分子构象变化的快相速度常数与失活速度常数基本一致，因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

If you want to live at will, you can only live commonly; if you want to live resplendently, you can only live paintfully;if you want to live long, you can only live simply........

你要活得随意，你就只能活得平凡；你要活得辉煌，你就只能活得痛苦；你要活得长久，你就只能活得简单

To help people live longer is one side or the other: tea sage Lu Yu lived for 72 years, tea Jiaoran monk lived for 81 years,"50 kg of tea," the monk lived for more than 130 years old, Qianlong lived for 88 years,"the world of tea " The 82-year-old Yuan Mei live.

助人长寿是其中一端：茶圣陆羽活了72岁，茶僧皎然活了81岁，"五十斤茶"和尚活了130多岁，乾隆活了88岁，"尝尽天下之茶"的袁枚活了82岁。

In this research, the activities of CWDE were measured by saccharifying according to the theory that red-brown amino compounds were produced when 3, 5-dinitro salicylic acid and aldehyde were heated together. And the testing conditions were made clear. The activity of Cx should be tested with optimal temperature of 50℃, optimal pH of 4.8, incubation time of 30 min, and optimal substrate of 0.6% CMC-Na. Due to the heat-libality, Cx should be sterilized more than 10 min. The testing conditions of the activity ofβ-G were almost the same with Cx except the substrate of 0.5% salicin and the sterilization time of more than 15 min.

主要取得了一下成果：(1)明确了条锈菌细胞壁降解酶活性测定的条件：羧甲基纤维素酶活性的测定条件为50℃，pH4.8，反应时间30min，底物浓度为0.6%羧甲基纤维素钠，以DNS法测定，且失活酶液的灭活时间应大于10min；β-葡萄糖苷酶活性的测定条件为：50℃，pH4.8，反应时间30min，底物浓度为0.5%水杨苷，以DNS法测定，且失活酶液的灭活时间应大于15min；果胶酶活性的测定条件为：50℃，pH4.8，反应时间1h，底物浓度为0.25%果胶，以DNS法测定，且失活酶液的灭活时间应大于10min。

Some people believed that, is living is for oneself joyful, therefore they to one's heart's content seek pleasure; Also some people believed that, therefore is living is can live well for the periphery person is living they with every effort for other people, makes the contribution for the society; Also some people believed,The human lives th is in order to know why oneself is living, therefore they seek unceasingly, discovery.

有的人认为，活着是为了自己快乐，于是他们纵情寻欢；也有的人认为，活着是为了周围的人能够更好地活着而活着于是他们尽力为他人，为社会做贡献；还有的人认为，人活一世就是为了知道自己为什么而活着，于是他们不断求索，发现。

When the bottom plate side syndiospecific lower than a sizzling, drawing pressure ball left, paper will go on there which would in turn low-speed machines brace will receive the same fault because plate following fulcrun is the eccentric shaft, corrected, you can add the eccentric shaft, convex to reverse some of the panels to fan-shaped with a sizzling plane are equal to the maximum State, low-speed machines la along two concave iron support, you can set the concave iron spare, Planer to some, is equal to the thickness to brace the distance below the iron plate plane, the installation at a later time.

当侧规底部扇形不活矮于铁不活立体时，不推纸压球压下去此后，纸弛不离会出不隐凹下下去不隐象，矮速机的不推条也会出不隐同样的障碍，因为扇形不活底下的支点是公道小轴，在校侧时，可以不兵公道小轴的凸背回旋一些，调度到扇形不活背同铁不活立体不等为最好状况，矮速机的不推条是由二块凹形铁撑持，可以不兵凹形铁拆上去，用刨床刨去一些，刨去的厚度即是不推条矮于铁不活立体的间隔，安设此后再举不静调度。

Abstract］ objective for the purpose of scientific evaluation and improvement on unreasonable parameter of viral inactivation,the dynamics curve of time corresponding to effect by given viral inactivation factor treated to blood plasma product were analyzed.methods aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with ph 4, human immunoglobulin and human hepatitis b immunoglobulin for intravenous injection with ph 4/pasteurization,treatment on human fibrinogen and human coagulation factor ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.the mean and standard deviation also coefficient of variation for virus survival titer in different time were stated, dynamics curve of virus inactivation were made and analyzed.results human albumin pasteurization inactivated vsv and sindbis virus,hrig low ph inactivated vsv and sindbis virus,hrig low ph inactivated hiv virus,ivig low ph/pasteurization inactivated hiv virus,ivig low ph inactivated hiv virus,hbig low ph/pasteurization inactivated vsv,sindbis and polio virus.conclusion suggestion about improvement on unreasonable parameter and standard of virus inactivation is made,cultivate target virus directly from final product by appropriate cell or method to monitoring viral safety of blood plasma product is advanced.

目的 分析血液制品特定灭活因子的时效动力学曲线，科学性地评价与改进不合理的病毒灭活参数。方法系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白蛋白采用巴氏消毒法，狂犬病人免疫球蛋白采用低ph常温孵放法，静注人免疫球蛋白、静注人乙肝免疫球蛋白采用低ph/巴氏消毒法；人纤维蛋白原、人凝血因子ⅷ采用有机溶剂/去污剂与干热法灭活病毒的验证资料，统计3批样品多个取样点的残余病毒滴度均值、标准差与变异系数，制作灭活病毒动力曲线图并进行分析。结果人血白蛋白巴氏灭活vsv与sindbis病毒，hrig低ph灭活vsv和sindbis病毒，hrig低ph灭活hiv病毒，ivig低ph/巴氏灭活hiv病毒，ivig低ph灭活hiv病毒，hbig低ph/巴氏灭活vsv、sindbis与polio病毒。结论建议改进病毒灭活验证标准与不合理的灭活参数；采用终产品样品通过合适细胞或方法直接培养目标病毒来有效监测血液制品的病毒安全性。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。