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Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点,我们对文献方法进行改进:文献方法用氢氧化钠与3,5-二甲基-4-羟基苯甲酸反应制得3,5-二甲基-4-羟基苯甲酸钠,再用3,5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3,5-二甲基-4-羟基苯甲酸苄酯,我们用氢氧化钾代替氢氧化钠,使合成取得成功;在合成2',6'-二甲基-4'-苯基-吖啶-9-甲酸酯时,文献方法对粗产品进行两次硅胶柱层析纯化,第一次柱层析用氯仿/乙酸乙酯(4∶1,V∶V)作溶剂和淋洗剂,第二次柱层析用己烷/丙酮(2∶1,V∶V)作溶剂和淋洗剂,按照文献方法得到的不是所需要的化合物,我们只进行第一次柱层析纯化,然后用己烷/丙酮(2∶1,V∶V)进行研磨,过滤,洗涤,除去溶于己烷/丙酮(2∶1,V∶V)的部分,得到所需要的产品。

A series of antimony mercaptide compounds which formula are Sb 〓 and Sb S (CH〓〓COOR 〓(R represents an alkyl or aryl, aralkyl, n=0~2) have been synthesized. A new synthetic method has been invented to solve the defect of abroad patent method which has complex technological conditions and singleness intermediate compounds. A new catalyst QL-1 and preactivated antimony trioxide and other means have been used to avoid neutralizing, washing, separating and other procedures of intermediate and simplifing process flow at the same time. According to this new technique, a series of antimony mercaptides and their intermediate products have been synthesized with high yield.

合成了结构为SbS(CH〓〓COOR〓及Sb〓(R为烃基或芳烷基,n=0~2)的一系列硫醇锑化合物,针对国外专利报道的合成工艺存在着工艺条件苛刻,同时需多步完成,操作复杂,而且中间体品种单一的缺陷,发明了一种一步法合成硫醇锑化合物的新工艺,采用QL-1催化剂,且将三氧化二锑预先活化,变固—液相反应为液—液相反应,同时可免除中间体的中和、洗涤、分离等工序,工艺流程简化,产品收率高,且能制得系列硫醇锑产品。

The preparation process includes the following steps: mixing 10 mM concentration water solution of chloropalladic acid and 12.5-25 mM concentration water solution of ammonium hexadecyl trimethyl bride in the volume ratio of 1 to 20, heating to 65-95 deg.c, adding 100 mM concentration water solution of ascorbic acid in the volume ratio to the water solution of chloropalladic acid of 0.16 to 1 to react for 30 min, centrifugal separation to collect precipitate, water washing, and drying to obtain cuboidal nanometer Pd material.

该钯纳米长方体材料的制备方法的步骤和条件法如下:把体积比为1∶20的10mM氯钯酸水溶液和12.5-25mM十六烷基三甲基溴化铵水溶液混合,加热至65-95℃,然后加入与10mM氯钯酸水溶液的体积比为0.16∶1的100mM抗坏血酸水溶液,反应30分钟,将溶液离心分离,收集到的沉淀用水洗涤,干燥,得到钯纳米长方体。

It achieves a certain extent effect of waterproof and oilproof. 3. Test the change of surface ingredient before and after the fluoridized of the FRP usingthe reflecting method of infrared spectrum. Then dip the fluoridized FRP in the cold water, warm water and petroleum ether for about half an hour, and surface ingredient havent changed too much.

用红外光谱反射法测试了经含氟物处理前后的玻璃钢表面结构变化,并且分别测试了在冷水、温水、石油醚中浸泡洗涤半个小时后的表面含氟处理的玻璃钢的表面结构,发现其表面结构与浸泡前无大的变化。

Sulfonic-4-(β-sulphatoethyl-sulphone) phenylamine is often applied to producing bifunctional-soluble reactive dyes and is an important dye intermediate. It plays an important role in the synthesis of KN style reactive dye M style double reactive dye and modified dissimilar double reactive dye. Used 2-sulfonic-4-(β-sulphatoethyl-sulphone) phenylamine as raw material can improve the uniformly dying performance and scourablility of dyestuff molecules and can improve the firm degree of colour staining.

2-磺酸-4-苯胺是制备水溶性双活性染料的重要中间体,在KN型活性染料、M型双活性染料以及改性异种双活性染料的合成中起着重要的作用,用2-磺酸-4-苯胺作原料合成活性染料可增加染料分子的匀染性和易洗涤性,提高染料的粘色牢度。

It is difficult to become skillful at determining phosphor in steel by the capacity method of ammonium phosphomolybdate,and the result of the determination is unstable.

针对采用磷钼酸铵容量法测定钢铁中的磷量这种方法难以掌握、测定结果不稳定的问题,对样品的溶解、沉淀的组成、洗涤液对沉淀组成的影响以及环境温度等方面进行了试验,对该方法加以改进,结果令人满意。

Specific products include: vacuum system cream machine, vacuum homogeneous emulsifying machine, mixer, reactor type, such as Cream, washing type, spices class production equipment; automatic aluminum composite hoses and plastic hose pipe production line, the kinds of specification type hose filling Sealing Machine, color filling Sealing Machine, hoses and other hose-type printing press production equipment; have liquid Filling Capping, capping machine, eye drops, oral liquid filling torinese plus spin to build machines and other types of packaging equipment bottle; have all kinds of liquid, cream, powder, granule packaging machine pouch; all kinds of stainless steel conveyor table; water treatment equipment; a variety of specifications for the pipeline system mold; stainless steel Clean Benches, cabinets, aircraft; soap production lines.

具体产品有:真空制膏机、真空均质乳化机、搅拌机、反应釜等膏霜类、洗涤类、调料类的生产设备;全自动铝塑复合软管和塑料软管的制管生产线、各种规格类型软管的灌装封尾机、彩条灌装封尾机、软管印刷机等软管类生产设备;有液体灌装旋盖、轧盖机,眼药水、口服液的灌装加内塞旋盖机等瓶类包装设备;有各类液体、膏霜、粉剂、颗粒的小袋包装机;各类不锈钢输送工作台;水处理设备;各种规格制管线模具;不锈钢超净工作台、柜、架;香皂生产线等。

Pregnancy ratewas significantly higher in women receiving 1 to 3 cycles of treatment than those receiving more than 3 treatmentcycles. Conclusion: Pregnancy rate after IUI was related to thickness of endometrium,time of IUI, diameterof fallopian tubal ampullae,density and motility of sperm after washing.

影响IUI结局的主要因素是:子宫内膜的厚度、IUI治疗的时机、输卵管壶腹部的直径、输卵管伞端距宫角的距离、洗涤后精子的密度和动力。

Vendors' responsibility - due to Wal-Mart strict requirements there will be monetary penalty if not compliance. Quality assurances; such as fabric testing for shrinkage, crocking, colorfastness, torque, correct labeling of fiber content, and care instruction for the product.

因为 Wal-Mart 的要求比较严格,像面料缩率,面料干湿摩擦色牢度,成衣扭力,面料成分,面料的纱支,面料的克重,面料的正确的洗涤方法等等,都要达到 Walmart 相应的标准,如果达不到要求就要被扣款。

Meanwhile, we discuss the deinking mechanism: solvent treatment dislodges most ink particles from the fibers, then ultrasonic treatment scatters detached ink particles which are separated from the stock by following washing and floatition process, so that deinking efficiency is improved greatly.

同时 我们对其脱墨机理进行初步研究,可以这样认为:先用溶剂预处理,将大部分油墨粒子从纤维上分离下来,然后再辅以超声波的作用,将分离下来的油墨粒子打碎,在后面的浮选洗涤过程中将这些打碎成很细小的油墨粒子去除,从而大大地提高了混合办公废纸的脱墨效果。

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