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At baseline and on day 1, 3, 7, 14, 21 following MPP+ injection, the degree of microglial activation was examined by detecting the immunodensity of OX-42 in the substantia nigra. The number of viable dopaminergic neurons was determined by measuring tyrosine hydroxylase positive neurons in the SN. Behavioral performances were evaluated by counting the number of rotations induced by apomorphine, calculating scores of forelimb akinesia and vibrissae-elicited forelimb placing asymmetry.

分别在基线、MPP+注射后第1、3、7、14、21天时通过测定黑质区域OX-42的免疫荧光强度评定小胶质细胞的激活程度,测定酪氨酸羟化酶的表达情况评定存活多巴胺神经元的数量;通过阿朴吗啡诱导的旋转行为、前肢跨步及触须引发的不对称放置试验得分测评行为学表现。

Once overdose gentamicin given can cause acute renal injury in rats. The increase of Hsp70 expression at earlier period suggested that the toxic drug of kidney can cause alarm reaction in epithelial cell of nephric tubulethe.Self-protective mechanism of cells might explain it.

一次大剂量注射庆大霉素可引起急性肾损伤,在损伤的早期HSP70的表达亦显著增加,提示肾毒性药物早期即可引起肾小管上皮细胞的应激反应,可能与细胞的自我保护机制启动有关。

Methods: Genes of MCP and DAF were connected by 10aa coding sequence of enriching Gly and Ser. Then insert into pcDAN3.1 vector, constituting pcDNA-MD3 expression plasmid of hMCP-DAF fusion gene. The genes were transformed by liposome into the pig endothelial cell; human serum handles masculine cloning, identify the function that restrain breaking of person alexin.

将MCP、DAF两个基因以富含Gly和Ser的10aa编码序列作铰链串接,并接入表达载体pcDNA 3.1中,构成含hMCP-DAF的真核表达质粒(pcDNA-MD3);以脂质体转染猪内皮细胞,人血清处理阳性克隆细胞,鉴定抑制人补体溶破的功能;应用显微注射将线性基因导入猪受精卵;PCR检测导入基因整合率。

They were injected intravenously with alloxan dissolved in saline solutions, at the dose of 240 mg/kg.

取16只作为正常对照组,不造成糖尿病模型,仅腹腔注射等量生理盐水。

D . The choice of experimental rats, 150 kg allyl chloride injection, once daily for 9d ays.

该实验选用雄性大鼠,皮下注射150 mg/kg 氯丙烯,每日1次,连续

The AME was produced by Alternaria alternata isolated from thegrain in an area of high incidence of esophageal cancer.

给动物腹腔内注射氚标记的交链孢酚单甲醚(~3H-AME),然后在不同时间测各脏器的放射性。

AIM To study the feasibility of establishing Alzheimer′s disease rat model with aluminum chloride by intrathecal injection.

目的 探讨鞘内注射AlCl3建立阿尔茨海默病大鼠模型的可行性。

In the early stage of STZ diabetic, administering BDNF protein into the vitreous cavities can increase TH protein levels and the density of dopaminergic amacrine cells in the STZ rats' retina.

在STZ糖尿病大鼠糖尿病早期,玻璃体腔内注射BDNF可提高视网膜中TH的水平,提高多巴胺能无长突细胞的数目。

RESULTS: The protein levels of TH, the number of positive staining dopaminergic amacrine cells and the staining gray scale of experimental group without BDNF were lower statistically.

结果:实验组糖尿病大鼠未注射BDNF眼视网膜中TH蛋白水平降低,多巴胺能无长突细胞计数及灰度低于对照组,均有统计学差异。

AIM: To observe the changes of tyrosine hydroxylase protein levels and the density of dopaminergic amacrine cells before and after the administering brain derived neurotrophic factor protein into the vitreous cavities of streptozotocin reduced Wistar diabetic rats.

目的:观察玻璃体腔内注射脑源性神经营养因子(Brain derived neurotrophic factor,BDNF)前后STZ糖尿病大鼠视网膜中酪氨酸羟化酶(tyrosine hydroxylase,TH)的水平及多巴胺能无长突细胞数目的变化。

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