注射

For parenteral products where there is a concern for pyrogens, it is expected that Water for I njection will be used.

对注射用药品来说，应重点关注热原物质，所以使用注射用水。

Oocyte samples from one group were collected to detect the presence and integration of HBV DNA within cells and chromosomes using PCR, Southern blot, dot hybridization and fluorescence in situ hybridization. The female animals from another group were mated with their normal males, respectively. Their zygotes, 2-cell embryos were collected to detect the integration of HBV DNA in the female pronuclei of zygotes and the replication and expression of HBV genes in the 2-cell embryos using FISH, RT-PCR and immunofluoresence assay.(1) PCR detected positive bands in the tested oocyte samples fromgoldon hamster and mice. Southern blot revealed clear hybridization signals in PCR products.

研究用金黄地鼠和小鼠建立实验动物模型：将卵巢内注射HBV DNA的实验动物分成两组，一组注射后进行超排卵，收集卵巢和输卵管的卵母细胞，用PCR、Southern杂交，斑点杂交和荧光原位杂交(fluorescence in situ hybridization、FISH)检测HBV在卵母细胞内的存在和染色体上的整合；另一组超排雌鼠与正常雄鼠合笼，收集受精卵和2-细胞胚，用FISH、RT-PCR和免疫荧光检测技术分别研究HBV基因在受精卵雌原核上的整合以及在2-细胞胚中的复制与表达。

Methods and Results Experiment 1: Intracytoplasmic injection of rat sperm heads was performed using 7~10 μm and 2~4 μm diameter pipettes and different injected method respectively.

方法和结果实验1：用直径为7~10μm和2~4μm的注射针以及相应的注射方法进行大鼠ICSI，ICSI后卵母细胞存活率(30.5% vs。 61.3%)和卵裂率(12.5% vs。 51.1%)均差异显著(P.05)；实验2：分别在hCG后14 h、16 h和18 h取卵进行ICSI，三组存活率77.4%、74.1% vs。

During the course of mice ICSI,some critical factors were realized on influencing the experimental results, including micromanipulation systems, diameters of injection pipettes, concentrations of PVP and positions of the first polar body located in oocyte atlCSL

另外，本研究论文还研究了一些对显微注射结果有影响的因素，包括操作液PVP液的浓度、显微注射时卵母细胞第一极体的位置以及显微操作系统等。

There were no significant difference in the respiratory rate, heart rates and temperatures among the three experimental groups. Conclusion Plateau pikas can be anesthetized with ketamine in a practical dosage from 22mg/kg to 44mg/kg by i.m. injection. Ketamine is safe and useful in anesthesia for plateau pikas.

盐酸氯胺酮行肌内注射麻醉是一种有效而安全的麻醉方法，适宜剂量为肌内注射22~44mg/kg体重，能为高原鼠兔的应用提供良好的麻醉效果。

Statistical results reveal that the cells contained met-enkephalin-immunoreactivite perikarya overlap the PHAL injection site and exhibit a similar morphology to PHAL-labeled cells in ICM.

结果发现，在中脑内缘区PHAL注射区存在脑啡肽(met-enkephalin， met-ENK)阳性免疫反应细胞，这些细胞和在ICM注射点中的PHAL标记细胞显示了相似的细胞形态。

Results Group C had the symptoms such as reduced activity, acceded, indulge in lying and weight loss after 3 weeks of immune injection, 14 out of 16 SD rats in Group B had the same symptoms as Group C after 4～5 weeks of immune injection, the serum enzymes in model groups increased significantly compared with those of the control group, model group C was much higher than model group B; the duration shorted, amplitude decreased, multiphase wave increased in electromyogram of model groups; MRI examination revealed samples from model group B and C had one positive case each, which presented T1MI isodensity or hypodensity signal, T2MI and STIR serial hyperdensity signal, revealing muscle inflammation; all rats'skeletal muscle from model group C and 11 out of model group B had pathological changes, which exhibited striated muscle focal fiber degeneration, necrotized and inflammatory cell infiltration, interstitial vessel wall thickening, random cardiac muscle samples had 3 positive changes, which had similar changes to skeletal muscle, there was 1 positive change from lung sample.

结果：模型C组于免疫注射第3周左右开始出现活动减少，倦怠嗜卧，食欲体重下降等表现，模型B组有14只SD大鼠于免疫注射第4～5周出现上述症状，较C组为轻；模型组肌酶谱中，肌酸激酶、乳酸脱氢酶、谷草转氨酶与对照组比较明显升高，模型C组较模型B组升高更显著；模型组肌电图时限缩短，波幅降低，多相波增多；磁共振检查模型B组和C组选送标本中各有一例有阳性改变，表现为T1MI等或稍低信号，T2MI及STIR序列为高信号，提示肌肉炎症水肿改变；模型B组有11只，模型C组全部大鼠骨骼肌出现病理改变，表现为横纹肌局灶性分布的肌纤维变性炎细胞浸润，间质小血管壁增厚、扩张，随机选送的心肌标本中有3例有阳性改变，表现与骨骼肌相仿，选送的肺标本中有1例有阳性改变，表现为蛋白渗出，炎症细胞浸润和小血管改变。

Spiral mandrel injection, injection valve, or two connected with the screw hole injection into the fixture, make it the top tight valve stuffing box.

用注射螺旋顶杆、注射阀或两个接在一起拧入夹具注射孔上，令其顶紧阀门填料箱。

The mice in experimental group and control group were exposed to 350 cGy radiation produced by 60Co. After 3 h, karyocytes at different concentrations in the fresh human umbilical cord blood were injected into the mice in experimental group A, B, C via their tail veins, and the equal volume of normal sodium was also injected into control group via tail veins. After one month, carbon tetrachloride (CCl4) was injected into experimental group A, B and control group via abdominal cavity, and the equal volume of normal sodium was injected into experimental group C. After two months, immunohistochemistry and reverse transcriptase polymerase chain reaction were used to detect the expressions of human cytokeratin-18 (CK18), cytokeratin-19 (CK19) and albumin in liver tissues of all mice.

采用60Co治疗仪γ射线对实验组和对照组行350cGy的亚致死剂量照射，实验组A、B、C3组照射后3h内经尾静脉分别输入1.0×10^7个/只、2.0×10^7个/只和3.0×l0^7个/只人新鲜脐血有核细胞，对照组经尾静脉注入等体积无菌生理盐水。1个月后对实验A、B组和对照组裸鼠经腹膜腔注射四氯化碳(CCl4)，实验C组注射等体积生理盐水。2个月后采用免疫组化和RT-PCR方法检测裸鼠肝组织人源性CK18、CK19和人源性白蛋白的表达。

Methods The present study has been carried out using NaCMC injected into the mice's abdominal cavity to cause inflammation and induce white blood cell migration,varnishing DNFB to induce mice's DTH,injecting colloid activated carbon suspension into mice's tail vein,with 10% of sheep RBC injected into the abdomen to immunize mice.We observed inhibitory function of Liyankang granule on NaCMC to cause inflammation and induce white blood cell migration,the effect of DNFB on inducing mice's DTH and the effect on clearance speed of carbon particle in mice's blood and serum agglution of mice.

采用大鼠腹腔注射羟甲基纤维素钠致炎诱发白细胞游走，涂抹二硝基氟苯诱导小鼠迟发型超敏反应，小鼠尾静脉注射胶体碳悬液，腹腔注射10%羊红细胞免疫小鼠，观察小儿利咽康颗粒剂对羟甲基纤维素钠致炎诱发白细胞游走的抑制作用，对二硝基氟苯诱导小鼠迟发型超敏反应、对小鼠血中碳粒清除速度、对小鼠血清凝集素的影响。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！