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The results showed that these contents decreased far below normal values at first as the mice were stimulated by noise; and sinbce then, gradually recovered to normal. And the effect of abdominal injection with Astragals was enhanced as the time of exposure to noise prolonged; while that of Rhodioda weakeded with higher noise intensity.

试验结果显示,大鼠受到噪声刺激后肝糖原含量起初远远低於正常值,而随著时间的延长逐渐接近正常值;腹腔注射黄芪的效果因噪声时间的延长而增强;而腹腔注射红景天效果因强度的增加而稍有减弱。

The period of treatment was fifteen days and every three month dogs were given a period of treatment. Berenil is the medicine to canine babesiosis, so dogs were given 3mg/kg b.w.

犬巴贝斯虫病的病原对驱虫药贝尼尔敏感,因而给全场犬肌肉注射贝尼尔2次,隔一星期注射一次,用量为3mg/kg b.w。。

Effects of Bushen Pill on Apoptosis Genes of Spiral Ganglion Cells Guinea Pigs with OtotoxityWANG Feng, ZHAO WulanZhejiang Chinese Medical University, Hangzhou 310053, China Abstract:ObjectiveTo explore the protective effects of Jingui Shenqi Pill on apoptosis genes of spiral ganglion cells in guinea pigs with ototoxity by gentamycin; MethodsThe ototoxity model was induced by abdominal injection of 100 mg/ gentamycin,while the treatment group took Jinguishenqi pill after injection of gentamycin, executed after the experiment, and the cochleas were gathered for detection.

作者:王枫,赵乌兰作者单位:浙江中医药大学,浙江杭州 310053 目的探讨金匮肾气丸对庆大霉素造成药物性耳聋豚鼠耳蜗螺旋神经节细胞凋亡调控基因表达的影响。方法豚鼠腹腔注射庆大霉素100 mg/制作耳聋动物模型,治疗组在注射庆大霉素后灌服金匮肾气丸水煎剂,实验结束后豚鼠断头处死,取耳蜗标本。

The objective was to determine the effects of active immunization of T-3-BSA in Rom cockerels, 400 cockerels were divided into 4 groups, group 1,2 and 3 were treated with T-3-BSA on different dose(0.2,0.4,0.8mg/mI respectively),within each group,there were 5 treatments following different vaccination schedules, group 4 served as controls. Every injection of immunogen was conducted on different sites around the neck, and the datas were measured at week 11,15,19, combs,wattles,body weight,serum T concentration,carass traits and testicular development and histology were studied in order to find out the most reasonable vaccination schedule and the most possible mechanism in the H-P-G axis of T.

本试验测定了睾酮—3—牛血清白蛋白(T-3-BSA)主动免疫对公鸡性腺与冠垂发育及雄激素水平的影响。400只罗曼蛋用雏公鸡除随机取25只作对照组外,另375只随机分成3大组,每大组125只再随机分成5小组,每小组25只,3大组分别使用0.2mg/ml、0.4mg/ml和0.8mg/ml的T-3-BSA免疫注射,每大组的①②③小组分别于3、6、9周龄免疫注射,④小组于3周龄首次免疫,6周龄加强免疫,⑤小组于3周龄首次免疫,9周龄加强免疫。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记,对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml),以不同扫描序列T1WI,T2WI,T2*WI(GRE进行MR成像,再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像,并测量信号强度,进行统计学分析。3缺血再灌注肾损伤模型建立和处理,然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只:注入标记细胞者10只,注入未标记细胞者6只),两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照,然后对收集的信号强度进行统计学分析。

Methods We treated 785 patients with medically intractable focal dystonia and muscle spasm in two groups, 192 cases with botox and 593 cases with CBTX-A. A total of 1 393 treatments were given over a 4-year period.

4年间用botox和CBTX-A分别注射治疗192和593例局限性肌张力障碍和肌肉痉挛等运动障碍性疾病,共计785例,注射1 393次。

The results showed that:(1)The proliferation activity of splenie lymphocytes stimulated by Con A was significantly increased with microinjection of Leu一enkephalin I p1(18 mmol/L) or Met-enkephalin I ucl(18 mmol/L)into bilateral hippocampus,but was inhibited by Lipopolysaccharide 1 41(50 ng/ul).

结果发现:(1)双侧海马内微量注射甲硫一脑啡肽或亮一脑啡肽各1 u1 (18 mmol/L)能明显增强Con A刺激的脾淋巴细胞增殖活性,而双侧海马内微注射脂多糖各1 }t1 (50 ng/PI)则起抑制作用。

Materials and Methods 20 New-Zealand White rabbits were divided randomly into 2 groups. Complete Freund adjuvant was injected into the left dorsal footpads of 10 rabbits so as to set up ipsilateral lymphadenitis model, while the right legs were measured as normal control. The left 10 rabbits received a subcutaneous implantation of VX2 tumor cell suspension (4×10~7 cells/ml) in fight thighs.

材料与方法:20只健康新西兰白兔随机分为两组,分别经左侧足垫注射完全弗氏佐剂建立淋巴结炎性增生模型和经右侧股四头肌注射VX2癌细胞悬液(4×10~7/ml)建立肿瘤转移淋巴结模型,以炎症组的对侧下肢不作任何处理作为正常对照。

Methods1 EAE was induced by being injected guinea pig spinal cord homogenatein complete Freunds adjuvant in the four footpads and different concentration bordetella pertussis vaccine in one footpad .2 The female wistar rats were randomly divided into control group, EAE groupand Tri group.

材料与方法 1。采用四肢足掌皮内注射豚鼠全脊髓匀浆-完全福氏佐剂,并辅以注射浓度为1×10~(11)个菌体/ml或1×10~(10)个菌体/ml的百日咳疫苗,诱导大鼠建立EAE模型。

The mold structure and working principle were introduced in detail, mainly stated in detail design of angle pin and pin gate .

通过对照相机前盖的使用要求以及工艺性分析,介绍了基于Pro/E注射模设计基本流程,并详细介绍了该注射模的结构和过程,重点阐述了斜销及点浇口设计要点。

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