注射

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

Six hundred ova were transfered into twenty-nine pseudopregnant recipients. Fifty-five offspring were born from the ova mircroinjected with the Bcl-2 fragment expression cassette.Twelve of them were proved to be gene integration positive by PCR .Four founder mice,two male and two female ,were further confirmed to be transgenic by Southern hybridization.

用BglII+SalI+PvuI三酶切pWBS和用BglII+BamHI双酶切pWCS，分别回收并纯化3.0kb的基因片段WAP-Bcl-2-SV40polyA和3.5kb的基因片段WAP-c-myc-SV40poly，作为目的基因，通过受精卵显微注射技术，分别导入C57BL／6J雌鼠与DBA／2J雄鼠交配的F1代小鼠受精卵的雄原核中，各注射约1000枚，将存活的受精卵各600枚分别移植至29只假孕母鼠输卵管壶腹部内。

Methods 38 cases with Peyionie s disease were studied. All patients accepted radiotherapeutics with total dose12~16 Gy, and injected with cortisone acetate injection 1mg and lidocaine hydrochloride injection 1.0ml (0.8ml into the mass and the rest around it). Results 35 cases were follow-up studied.

38例阴茎海绵体硬结症患者，均予直线加速器放疗，总剂量12~16 Gy，同时给予醋酸可的松1 mg+2%利多卡因1.0 ml硬结内注射0.8 ml，其余注射到硬结四周。

Methods The CEA ScFv was radioiodinated (125I,131I) by iodogen solid labelling method.The uptake of radioactivity in tumor and normal tissues in 20 human rectocolonic tumor bearing nude mice was measured at 1,3,6 and 24 hour after peritoneal injection of 125 I-CEA ScFv and in 3 human rectocolonic tumor bearing nude mice imaging was carried out at 1,3,6 and 24 hour after peritoneal injection of 131 I-CEA ScFv.

采用Iodogen法制备125I-CEA ScFv和131I-CEA ScFv.20只荷人结直肠癌裸鼠，腹腔注射125I-CEA ScFv后1、3、6和24 h进行裸鼠的体内分布研究，对3只荷人结直肠癌裸鼠腹腔注射131I-CEA ScFv后1、3、6和24 h进行裸鼠的定位显像研究。

The literature about the synthesis of the ion exchange and chelating adsorbents used in the off-line separation and preconcentration of the noble metals and the development of the on-line microcolumn separation and preconcentration of the noble metals were reviewed. Four silica gel-based adsorbents with rhodanine, 2-mercaptopyrimidine, 2-mercaptothiazoline and isophenylthiourea as the functional groups were first synthesized and applied to the on-line separation and preconcentration of silver, gold, palladium and platinum for their FAAS determination.

本论文在回顾螯合吸附材料的合成及其在贵金属的离线和流动注射在线预浓集分离发展状况的基础上，首次合成了含罗丹宁、2-巯基嘧啶、2-巯基噻唑啉和异二苯基硫脲等四种以硅胶为载体的螯合吸附剂，并用于银、金、钯和铂的流动注射微填充柱在线预浓集分离、火焰原子吸收光谱分析。

Methods: Microinjection technique was used to structure the human SERT heterologious system.

显微注射方法将 hSERT 基因 cRNA 注射到非洲爪蟾卵母细胞内，建立异体表达系统。

Abdominal writhing behaviors were monitored in 45 minutes as indicators of pain. The serumal TNF and IL-6 were tested by radioimmunology.

其余大鼠分别于注射BTX-A后1周、4周、8周各组随机取6只，腹腔内注射乙酸(2％，3 ml／kg)，观察大鼠扭体反应45min，并进行行为学评分和比较。

RESULTS: The mucosal damage was slighter in the experimental group than that in the control group.

睡眠剥夺期间每日早8：00实验组腹腔注射MT（15 mg/kg），对照组腹腔注射等体积生理盐水。

Methods To study the influence of microwave heating to the determination of sodium gluceptate and stannous chloride for injection,and compare with water bath heating method.

目的 微波加热测定注射用亚锡葡庚糖酸钠中葡庚糖酸钠的含量方法考察微波加热对注射用亚锡葡庚糖酸钠含量测定的影响，并与水浴加热进行比较。

Then rAAV-DJ-1 was transfected into HEK-293 cells for packaging. AAV vectors encoding human DJ-1 protein were stereotactically injected into the rat SN 4 weeks prior to rotenone lesion in SN. Tyrosine hydroxylase protein was determined using immunohistochemical staining. Ethovison software allowed measurement of movement distance in 30 minutes.

并转染HEK-293细胞，进行腺相关假病毒颗粒包装，所得病毒颗粒注射到大鼠脑内感染黑质神经细胞；4周后，注射鱼藤酮于大鼠黑质相同脑区；通过免疫组织化学方法鉴定TH蛋白表达情况，采用动物行为轨迹分析软件计算大鼠30 min内运动距离。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！