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Methods: The rats were injected with thioacetamide 600mg/kg twice at same time for two days to induce the model of acute hepatic failure rat except the normal group injected with the normal saline hypodermically.

按600mg/kg的剂量经皮下注射硫代乙酰胺1次,24小时后按相同剂量重复注射1次,造成大鼠急性肝衰竭模型,于实验前3天至实验结束分别给予各组大鼠生理盐水,不同剂量丹黄方及促肝细胞生长素,观察造模后48小时内大鼠病死率,并于造模48小时后经腹主动脉采血测定ALT、AST、TBil、TNF-α、IL-6,取肝组织作病检。

At the one-cell stage, each embryo was injected with gradient doses of 2-8ng MO. The control morpholino, was purchased from Gene-Tools.To test the knockdown effectiveness of the morpholino, the tbx2-EGFP (enhanced green fluorescent protein) construct was generated. And coinjection of morpholino with mRNA tests its specificity of the morpholino. Overexpression of tbx2 via mRNA microinjection helps us understand gaining function of tbx2. Results The tbx2-EGFP construct tests the knockdown effectiveness of the morpholino. Zebrafish embryos lacking tbx2 function have defects in cardiac contractility, rhythm and morphology in a dose-dependent manner. The embryos with c-MO are normal in development. Coinjection of morpholino with mRNA rescue the phenotype of tbx2 knocking down. The phenotypes include pericardial edema, hypogenetic ventricle, dilation of atria, arrhythmia, bradycardia, asystole, abnormal atrioventricular canal, aberrant valve and blood regurgitation.

结果荧光蛋白融合标记实验验证了tbx2-MO可以很好得阻抑斑马鱼胚胎tbx2的表达,tbx2-MO组胚胎在心脏收缩性、节律和形态学发生上出现了异常,心脏畸形随着注射剂量的增加而出现比较一致的表型,而c-MO组胚胎没有出现明显的畸形;tbx2-mRNA和tbx2-MO的共注射减少了tbx2-MO胚胎心脏畸形的发生率及减轻了心脏畸形的程度,验证了所设计合成的tbx2-MO对tbx2基因的抑制作用具有特异性;tbx2-MO组胚胎心脏缺陷包括心室发育不良、心房扩张、房室管和瓣膜异常以及心率缓慢、心律不齐、心脏停搏、血液返流等,并根据心脏发育异常的程度分为轻、中、重度畸形组tbx2-mRNA基因过表达胚胎没有见到心腔的形成。

The rats' infraorbital nervous tracts were exposed during test. 0.5% Adriamycin was directly injected into the axons, while normal saline into the opposite site as control.

本实验通过手术解剖暴露大鼠眶下神经束,用0.5%阿霉素直接神经干内注射,对侧用生理盐水注射作自身阴性对照。

That indicated the local effect of renal intracapsular injection SM.The result of this study gave us an e...

提示肾囊注射丹参的局部作用,为肾囊注射丹参治疗肾脏疾患提供了实验基础。

Results There was a 90%cure rate in the intramuscular injection groupand a 94%cure rate in the intracervical injection group.

结果 两组患者年龄和一般资料无显著性差异,肌肉注射组和宫颈注射组治愈率分别达90%与94%,无显著性差异。

Observing localization of the drug and morphologic change in DRG cells and motor neurons in spinal cord anterior hom after 1% adriamycin intraneural injection into the sciatic nerve of rabbits. Moreover, evaluating sensory and motor function 1-8 weeks following injection. Exploring whether the agent, injected into sciatic nerve, has the effect of chemo-ganglionectomy on the corresponding DRG.

研究家兔坐骨神经内注射1%阿霉素后,药物的聚集部位及相应脊髓前角运动神经元和背根节细胞的形态学变化,测定家兔1~8周下肢感觉及运动功能,探讨坐骨神经内注射阿霉素能否对相应背根神经节产生化学切除术作用。

Methods: 252 patients with tuberculous pleuritis were divided into two groups by random allocation. Urokinase group: 126 cases received intrapleural injection with urokinase 5×10^4 U adding into 0.9% sodium chloride injection 20 ml after each thoracic drainage, and non-Urokinase group: 126 cases received intrapleural injection with 0.9% sodium chloride injection 20 ml after each thoracic drainage.

将252例患者随机分为两组,尿激酶组126例,用0.9%氯化钠注射液20 ml加尿激酶5万U于每次抽液后行胸腔内注射;非尿激酶组126例,用0.9%氯化钠注射液20 ml于每次抽液后行胸腔内注射,治疗结束后进行比较。

Treatment group:35 cases treated by intrapleural administration of OK-432(10KE) plus DDP(80mg) with negative penicillin skin test.

治疗组 (35例):以青霉素皮试为阴性,取沙培林 10KE ,顺铂 80mg胸腔内注射;对照组(34例):单用顺铂 80mg胸腔内注射

After drainage as possible as, treatment group were treated with intrapleural injections of KLT plus DDP and thermotherapy,control group were treated with intrapleural injections of DDP, to observe side effects during treatment and the changes of Pleural Effusion and Pleura after 1 month.

将60例恶性胸水患者随机分为两组:治疗组和对照组,均行胸穿,尽量引尽胸水后,治疗组采用康莱特与顺铂胸腔注射联合热疗,对照组仅用顺铂胸腔注射,观察治疗期间副反应和1个月后患者胸水及胸膜变化情况。

Objective To evaluate the efficacy and safety of intraprostatic injection of chuanshentong in the treatment of chronic non-bacterial prostatitis/chronic pelvic pain syndrome.Methods 56 patients with diagnosed CAP/CPPS, 6ml of mixed solution of chuanshentong was transperineally injected into one lobe of the prostate, once a day for 8 days.The efficacy was evaluated by the NIH chronic prostatitis symptom index. The main semen parameters before and treatment were compared.

目的 评价经会阴注射川参通治疗非细菌性列腺炎/慢性骨盆疼痛综合征的有效性和安全性方法将56例诊断 CAP/CPPS的患者运用川参通6ml,经会阴注射至前列腺,每日1次,共8次;采用美国国立卫生研究院制定的前列腺炎症状评分标准判定疗效,比较治疗组治疗前后精液的主要参。

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