注射

GdCl3(10mg/kg) or the same volume of NS was continually injected of vein at 48h and 24h before LPS(5mg/kg) was injected in the male kunming species mouse. Then took out of liver or isolated KCs 30 minute after LPS was injected and assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of liver or KCs in vivo.Ⅱ. Isolated and cultured KCs of mouse, 1 h pretreatment with GdCl3; Culture medium with LPS(100ng/ml) was added and continuatively incubated 30 minute. Assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of KCs in vitro and detection effect of GdCl3 on its phagocytosis function in respectively.

Ⅰ 雄性昆明种小鼠在注射LPS（5mg/kg）前48h、24h，分别静脉注射GdCl3（10mg/kg ）或等量的生理盐水，于LPS或生理盐水注射后30min，分别取出肝脏或分离KCs，检测肝脏或KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平；Ⅱ体外培养小鼠KCs经GdCl3（100μM）预处理1h，加入含LPS（100ng/ml）的DMEM培养基继续孵育30min，分别检测体外培养KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平和GdCl3对其吞噬、分泌功能的影响。

The positive ratio through injecting the foreign gene to the sperm reservoir is higher than to that through injecting it to the copulatory pouch.

将外源DNA注射进雄蛾贮精囊比注射到雌蛾交配囊后代有更高的阳性率，且同蛾区嵌合现象轻些。

INTERVENTIONS: The animal epileptic model was set up by intra-abdominal injection of coriamyrtin 15 mg/kgprovided by Pathology Department of Harbin Medical University. Convulsion presented in all of rats, 5 minutes later after injection, lasting for 1 or 2 minutes.

干预：按1.5 mg/kg腹腔注射马桑内酯(由哈尔滨医科大学药理学部提供)建立动物癫痫模型，全部大鼠于注射后5 min出现抽搐，持续时间一两分钟。

In the present study,in order to explore the roles of estrogen and progestin in epilepsy and elucidate their mechanisms of action, we utilized animal seizure models induced by intracerebroventricular coriaria lactone and intraperitoneal bemegride, studied the effects of estrogen and progestin on central nervous system functions in behavior,electrophysiological,cellular,molecular and gene levels by means of neuroelectrophysiology, flow cytometry, high performance liquid chromatography, immunocytochemical and in situ hybridization techniques.

为了探索雌、孕激素在癫痫发病中的作用，阐明其作用机制，本工作分别以马桑内酯侧脑室注射致痫、贝美格（Be腹腔注射致痫大鼠为实验对象，采用神经贫血电生理、流式细胞美白免疫荧光、高效液相色谱、美白免疫细胞化学、原位杂交早泻技术，从整体、行为、细胞、分子以及基因水平研究发现了雌、孕激素对大鼠中枢神经贫血系统功能的影响。

200IU BTX-A was dissolved in 8ml normal saline, and the solution of BTX-A was injected into 8 different points in urinary sphincter using a flexible cystoscopic needle, with each point of 1ml solution. The effects was evaluated and followed up. Result: One month after injection, the hydronephrosis decreased from 3.9±1.2cm to 1.1±0.8cm and PUV did so from 187±58ml to 54±18ml in all patients.

对逼尿肌－外括约肌协同失调的13例患者，将200IU BTX-A溶解于8ml生理盐水，在膀胱尿道镜下分点注射于外括约肌内；对DESD合并逼尿肌－膀胱颈协同失调的6例患者，将200IU BTX-A溶解于12ml生理盐水，分点注射于膀胱颈及外括约肌内。1个月后复查上述检查指标。

After controlsomatosympathetic reflex responses were obtained, midazolamwas administered IV to four groups of randomly allocated catsas follows: brain-intact cats at a dose of 0.03 mg/kg, brain-intactcats at a dose of 0.1 mg/kg, brain-intact cats at a dose of0.5 mg/kg, and decerebrate cats at a dose of 0.1 mg/kg.

控制下的体－交感反射产生后，我们把猫随机分成四组，分别静脉注射如下剂量的咪唑安定：大脑完整的猫注射剂量分别为0.03mg/kg 、 0.1mg/kg 、0.5mg/kg；去大脑的猫注射剂量为0.1mg/kg。

In the experiment, the FSH+LH combinations of 4 doses were adopted to superovulate KM mice (FSH was injected by IP 4 times degressively, and LH by IP once.).

试验中采取四种剂量的FSH+LH组合(FSH分四次递减量腹腔注射，LH一次腹腔注射)对小鼠进行超排处理。

The water for transfusion injection is produced by using multi-effect water distillatory, namely to adopt repeatedly heat exchange to distill purified water, and to meet the standard of injection water.

大输液注射用水采用多效蒸馏水机生产，即通过多次热交换对纯化水进行蒸馏，以达到注射用水水质标准。

SC the neck and back of mice by D gal(120mg/kg) for 40days,SC the herbs group by D gal let them drink the concentrated two old drugs decoction for 40 dyas,and compared with the empty group,than observe the effect on the indices of the brain,dthymus,spleen,liver and kidney,the activity of SOD of serum,MDA and t...

小鼠颈背部皮下注射D 半乳糖 4 0天，中药组注射D 半乳糖同时以 6 32g/(kg·d-1 )浓缩二陈汤煎液灌胃 4 0天，并以空白组作对照，观察二陈汤对小鼠脑、胸腺、脾、肝、肾指数、自由基代谢指标血清超氧化物歧化酶、丙二醛及小鼠大脑额前页神经元超微结构的影响。

In essence, the heptamer is like a syringe loaded with edema factor and lethal factor, and the slight acidity of the endosome causes the syringe to pierce the membrane of the endosome and inject the toxic factors into the cytosol.

七聚体实质就像一管注射针筒，里面装满了水肿因子以及致命因子，而内小体的微酸环境则令注射针筒戳破内小体的膜，把有毒因子注入细胞质。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！