注射

Methodsbeauty in the face for the purpose of the 300 cases of patients with Botulinum Toxin Type A fixed-point, quantitative injection, the injection point uniform, facial symmetry in the distribution of muscle layer.

对以面部美容为目的的300例患者应用A型肉毒毒素定点、定量注射治疗，注射点均匀、对称分布于面部肌肉层。

objectiveto observe the botulinum toxin type a treatment of facial wrinkles using the results.methodsbeauty in the face for the purpose of the 300 cases of patients with botulinum toxin type a fixed-point, quantitative injection, the injection point uniform, facial symmetry in the distribution of muscle layer.

目的：观察a型肉毒毒素治疗面部皱纹的运用效果。方法：对以面部美容为目的的300例患者应用a型肉毒毒素定点、定量注射治疗，注射点均匀、对称分布于面部肌肉层。

In addition to the main injection, pre and post-injections are possible.

除了主要的注射，前，后注射是可能的。

One of the major points of contention has centered on the proposition in the United States to make the vaccine mandatory for preteen girls, as it is effective only when administered to women who are not infected with HPV.

由于疫苗仅对未感染HPV的女性注射有效，一个争议的主要观点集中在，美国政府对青春期以前女性强制进行疫苗注射的提议。

One of the major points of contention has centered on the proposition in the United States to make the accine mandatory for preteen girls, as it is effectie only when administered to women who are not infected with HP.

由于疫苗仅对未感染HP的女性注射有效，一个争议的主要观点集中在，美国政府对青春期以前女性强制进行疫苗注射的提议。

To make the transgenic mice: We purified the gene fraction nestin-hsp68-EGFP and injected it into pronucleus of mouse zygotes to generate the transgenic mice.

1。转基因小鼠的获得：纯化基因片段nestin-hsp68-EGFP，用显微注射方法把基因片段注射到受精卵的雄原核中，获取转基因小鼠。

Recombinant expression vector k14/c-myc-paav was constructed bearing the mouse c-Myc cDNA derived by the skin specific human keratin 14 promoter, then the linear vector was microinjected into the two pronucleus of the C57BL/6 fertilized eggs to produce transgenic mice.256 microinjected eggs were transferred, 6 of the foster mothers were pregnant and 44 adult mice were produced, two of them were positive transgenic mice confirmed by PCR and Southern blot analysis, transgenic rate was about 4%.

以CMV-PAAV载体为基础载体，以皮肤特异人K14（人角蛋白14）基因启动子替换原质粒中的CMV启动子后，在其多克隆位点区依次插入鼠c-Myc基因的cDNA序列和gfp基因的编码序列，构建了高效表达c-Myc基因的真核表达载体。通过双原核显微注射技术，将线性化的载体DNA注入到C57BL/6小鼠受精卵的两个原核中，然后将注射后状态良好的胚胎移植到代孕鼠的输卵管中，共移植了256枚受精卵，6只受体怀孕，最终获得44只成年小鼠。

We used Aeromonas hydrophila which we separated from the diseased Paralichthys olivaceus to made a inactivated whole cell bacterin .We injected this vaccine in the mice and the Paralichthys olivaceus .Both the mice and the Paralichthys olivaceus gained a good protection of 80% and 70%.The fish with dipping immersion gained a good protection of 85%,and the incidence of disease was 6%.We detected the antibody level in the antiserum by agglutination reaction and indirect ELISA which seemed to be immunogenic crucially.We anatomized the mice then we found that all the purtenance of the immunzied mice are healty.All of this proved that the vaccine is effective.

本实验将所分离到的嗜水气单胞菌用终浓度为0.2%~0.5%的甲醛对菌体进行灭活，制备了灭活疫苗，并优化灭活疫苗的灭活条件；将制备的灭活疫苗分别注射小鼠和牙鲆，均取得了较为理想的效果，其中小鼠的免疫保护率达到80%，牙鲆的免疫保护率达到70%；采用渗透压差法大规模浸泡免疫牙鲆也取得了很好的效果，浸泡免疫过的牙鲆发病率仅为6%，攻毒后免疫保护率为85%；通过应用间接ELISA方法检测受免动物的抗血清，并将牙鲆的抗血清注射新西兰白兔，从而获得兔抗鱼血清，更便于检测，结果都表明动物体内产生了较高的抗体水平；剖检攻毒后的动物，发现免疫过的动物内脏健康无异样，表明该疫苗安全有效，从而为防治牙鲆腹水症开辟了一条新途径，也为进一步制备其基因工程疫苗奠定了基础。

In conclusion, the 2 hours after quartic injection of IUdR and single injection of FMISO appears to be the optimal time for the combination of radiation.

而单次[18F] FMISO注射后2及4小时牺牲点的肿瘤放射活度差异不显著，因此注射后2小时进行放射治疗，应会有最佳的辐射增敏效果。

Moreover, using the intracerebral injection of LTA to evaluate the direct effect of LTA on developing microglia, we showed that ramified microglia labelled with OX-42 or ED-1 progressively retracted their processes then became amoeboid form, indicating that microglia were activated in response to LTA.

另外，利用脑内注射脂壁酸来探讨脂壁酸直接刺激小神经胶细胞的反应，结果显示在注射处有些受OX-42和ED-1标志的分枝状小神经胶细胞，渐渐的收回突起转变成圆形，显示小神经胶细胞有被活化的情况。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。