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M{rX*o0 OBJECTIVE: To investigate the effects of neuronal nitric oxide synthase inhibitor 7-nitroindazole (7-NI) on smoke inhalation pulmonary injury in rats. METHODS: Forty healthy male SD rats were randomly divided into three groups: control group (n=8), smoke inhalation model group (n=16) and 7-NI treatment group (n=16). After reproducing the smoke inhalation model, to the 7-NI treatment group rats 7-NI (20 mg/kg in 2 ml arachis oil) was administered by intraperitoneal injection, while in the control group and the model group, 2 ml arachis oil was administered by intraperitoneal injection.

探讨神经型一氧化氮合酶抑制剂7-硝基吲唑(7-NI)在烟雾吸入性肺损伤中的作用。40只SD雄性大鼠被随机分为正常对照组(n=8)、烟雾吸入性肺损伤模型组(n=16)和7-NI治疗组(n=16),建立烟雾吸入性肺损伤模型。7-NI治疗组在致伤后立即腹腔注射7-NI 20 mg/kg(溶于2 ml花生油中);正常对照组及模型组腹腔注射2 ml花生油。

After reproducing the smoke inhalation model, to the 7-NI treatment group rats 7-NI (20 mg/kg in 2 ml arachis oil) was administered by intraperitoneal injection, while in the control group and the model group, 2 ml arachis oil was administered by intraperitoneal injection.

探讨神经型一氧化氮合酶抑制剂7-硝基吲唑(7-NI)在烟雾吸入性肺损伤中的作用。40只SD雄性大鼠被随机分为正常对照组(n=8)、烟雾吸入性肺损伤模型组(n=16)和7-NI治疗组(n=16),建立烟雾吸入性肺损伤模型。7-NI治疗组在致伤后立即腹腔注射7-NI 20 mg/kg(溶于2 ml花生油中);正常对照组及模型组腹腔注射2 ml花生油。

Pathological studies also showed more tissue damage in the group with Fluorescence humic acid and arsenic injection than the group with Fluorescence humic acid injection only.

病理切片研究也发现注射萤光腐植酸加砷的一组,组织伤害远比只注射萤光腐植酸的一组严重,这也似乎表示萤光腐植酸的确有致病能力,而砷对萤光腐植酸的毒性则有加强作用。

With Fluorescence humic acid and arsenic injection, 4 out of 7 animals developed symptoms; with Fluorescence humic acid injection alone, 2 out of 7 animals developed symptoms, but all symptoms disappeared in 4-12 days even injections were continued. It seems, animals have the ability to recovery by themself.

注射萤光腐植酸加砷的一组,七只小白鼠中有四只产生症状,在只注射萤光腐植酸的一组,七只小白鼠中有两只产生症状,但是虽未停止投药,症状却在4-12天内完全消失,似乎小白鼠体内自有修复伤害的能力。

The mice in VEGF group were injected with exogenous VEGF into the caudal veins on day 7, 15, 30, 60 respectively after operation.

实验组小鼠分别于术后7、14、30、60 d于尾静脉内注射VEGF,注射后7、15、30、60、90 d取脾组织标本。

Tumefaciens mediated transformation using cotyledonary node as explants was optimized; 3 methods including cutting chapiter, ovary injection and chapiter spreading for pollen tube pathway was compared. Cutting chapiter and ovary injection can both achieve insect resistant gene tranformants.

优化了以子叶节为外植体的农杆菌介导转化技术;比较了切割柱头、子房注射和子房涂抹3种花粉管通道法处理授粉后黄瓜子房的转化效果,切割柱头和子房注射均可成功获得黄瓜抗虫转基因植株。

2Carry on histology observation and histochemical measurement on samples taken from operation of prolapse of lumbar intervertebral disc and operation after chemolysis failure.

注射用胶原酶1200u,溶于0.9%生理盐水2ml,在直视下将胶原酶溶液注射于硬膜外隙和椎间盘表面切开处各1ml;分别于不同的时间段处死实验动物,取标本进行组织学观察和组织化学检测。

Methods 18 rabbits were used to observe the osteogenesis of percutaneous autogenous bone marrow into cicatrix of bone defect.A 1cm bone defect model was made in each side of the radius.

选健康家兔18只,建立双侧桡骨中段骨及骨膜缺损1cm模型,两骨断端髓腔用骨蜡封闭。6周后,实验侧桡骨骨缺损区经皮注射自体红骨髓2ml;对照侧桡骨骨缺损区注射自身外周血2ml。

In the former group,28 rats were injected into the cisterna magna with 0.05ml volume of 2% kaolin solution under microscope.

实验组28只大鼠应用显微外科技术在显微镜下向枕大池中注射2%高岭土混悬液0.05mL,对照组12只大鼠应用显微外科技术在显微镜下向枕大池中注射生理盐水0.05mL。

In the first experiment, immature female rats were treated twice daily(8 IU/8 times) with recFSH into abdomen, and twice daily(8 IU/8 times) or daily(4 IU/4 times) or daily(8 IU/8 times) or every other day (8 IU/2 times) or only times (8 IU/1 times) with recFSH into cloaca for four days.

实验一,将未成熟的母鼠分别於腹部注射recFSH每天两次(8IU/8次)与泄殖腔各别注射每天两次(8IU/8次)、每天一次(4IU/4次)、每天一次(8IU/4次)、每两天一次(8IU/2次)与仅一次(8IU/1次),共连续四天。

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