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Results:The acetone body of all patients turned into negative, but the therapy time of therapy group was less than that in control group (P.05 ).The blood glucose levels of patients in two groups were all well controlled.

对照组小剂量普通胰岛素24 h持续静脉点滴,三餐前门冬胰岛素皮下注射,睡前合成胰岛素皮下注射,剂量从小剂量开始,以后随血糖水平调整。

SAP model in SAP group and Ani group was produced by the method of Aho et al.

模型制备采用Aho〓等方法,即胆胰管逆行注射5%牛磺胆酸钠,对照组则注射等量生理盐水。

METHODS: Adult male Wistar rats, 9 weeks of age, were injected intraperitoneally with STZ to create diabetes. At 2 week after the models were created, BDNF protein was administered into the vitreous cavities of rats. At 4 week after the models were created, the rats were killed and the retina was removed for Western blotting and Whole-mount immunohistochemistry for TH to observe the changes of TH and dopaminergic amacrine cells in retina.

9周龄Wistar大鼠,链脲佐菌素(streptozotocin,STZ)腹腔注射,制成糖尿病模型,于模型建立2wk后,大鼠眼玻璃体内注射BDNF溶液,于模型建立4wk后,处死大鼠,取出眼球,取视网膜组织进行Western blotting及视网膜铺片免疫组织化学染色检测,观察视网膜中TH水平的变化,从而反映糖尿病大鼠视网膜中多巴胺能无长突细胞水平的变化。

Results:①Excitation of the NVM by L-glut amate,injection of substance P into the nucleus dorsomedialis, n ucleus paraventricularis or rostral ventrolateral medulla,all induce d pressor responses.②NVM pressor responses could be attenuated by preinjection of [D-Pro2,D-Phe7,D-Trp9]-SP (DPDPDT,a substance P antagonist)bilate rally into the NDM,NPV or RVL,but preinjection of atropine into the RVL had no s uch effect.

结果 :①L-谷氨酸兴奋NVM、P物质注入背内侧核室旁核或延髓头端腹外侧区均引起升压反应;②NVM升压反应可被双侧NDM、NPV或RVL内预先注射[D- Pro2,D-Phe7,D-Trp9]-P物质衰减,但RVL内注射阿托品无此效应;③酚妥拉明i.v。

Carnitine injection is recommended at 1 ampoule every 2 days for best result.

-肉碱注射剂建议每2天注射1安瓿可以达到最好的结果。

Sixty Wistar rats were randomly assigned to receive sham operation, blockage of unilateral ureteral without treatment, or intraperitoneal injection of amygdalin at a dose of 3 mg or 5 mg, and then sacrificed on days 7, 14 and 21 postoperatively, Pathological changes in the kidney in each group were observed.

用单侧输尿管梗阻的方法建立大鼠肾脏间质纤维化模型。60只Wistar大鼠随机分为假手术组、单侧输尿管梗阻组、苦杏仁甙3 mg腹腔注射组和苦杏仁甙5 mg腹腔注射组,分别于术后7、14、21 d处死动物,观察各组大鼠肾脏病理损害程度。

In recreational use, it can be swallowed, snorted, smoked, dissolved in water and injected (or even without water, in what is called a dry shot), inserted anally (with or without dissolution in water; also known as a booty bump or shafting), or into the urethra.

娱乐目的的服用方式多种,可吞咽,鼻吸,烟吸,溶入水中后注射(不用水的话,成为"干注射"),插入肛门或尿道。

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要:通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt;采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中,然后将注射存活的受精卵植入假孕母鼠的输卵管;取其产3 wk龄子代鼠进行PCR初选,再通过Southern杂交进一步确证;利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

Methods:All of 40 male Sprague-Dawley rats were divided into 3 groups:control group(group A, n = 10), diabetic group for 4 weeks(group B, n = 14), and diabetic group for 12 weeks(group C, n = 16). The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NF-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组:A组为正常对照组(10只),B组为糖尿病4周组(14只),C组为糖尿病12周组(16只),采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后,A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能,并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达,同时采用电泳迁移率变动分析法进行NF-κB 活性检测。

Methods All of 40 male Sprague-Dawley rats were divided into 3 groups: control group (group A, n=10), diabetic group for 4 weeks (group B, n=14), and diabetic group for 12 weeks (group C, n=16). The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NE-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组:A组为正常对照组(10只),B组为糖尿病4周组(14只),C组为糖尿病12周组(16只),采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后,A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能,并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达,同时采用电泳迁移率变动分析法进行NF-κB活性检测。

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