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aim: to evaluate the feasibility of injectable calcium phosphate cement and fibrin sealant as an injectable compound carrier of bone morphogenic protein .

目的: 了解以注射型磷酸钙骨水泥/纤维蛋白胶共同作为骨形态发生蛋白复合载体的注射型骨修复材料在小鼠体内异位成骨的能力,探讨其作为注射性骨替代物的可行性。

Methods 32 cases of patients with myospasm after head injury were inpatiens of neurosurgery and convalescent ward from february 2004 to january 2007, each of them was intramuscular injected botulinum toxin type adeeply and symmetrically.

选取32例2004年1月至2007年2月在我院神经外科及康复科治疗的脑外伤后肢体肌肉痉挛病人,每例行国产a型肉毒毒素痉挛肌等距离多点均匀深部注射治疗,每次注射总量不超过100u,每次注射1-2块肌肉。

The nephridial tissue of all the rats was taken for pathological examinations.

选用健康雄性SD(Sprague-Dawley)大鼠,腹腔注射8 mL/kg的DEG,于注射前和注射后第2天、5天、7天以及14天尾静脉采血检测血肌酐、尿素氮和CO2结合率(CO2CP),收集死亡大鼠及第7天和14天处死大鼠的肾组织进行病理检查。

Healthy SD male rats were given DEG with the dose of 8 mL/kg body weight (intraperitoneal injection I.P.). The level of the serum Cr, BUN and CO2CP(CO2 Combining Power CO2CP)were tested before and after the injection the 2nd , 5th , 7th, and 14th day. The nephridial tissue of all the rats was taken for pathological examinations.

选用健康雄性SD(Sprague-Dawley)大鼠,腹腔注射8 mL/kg的DEG,于注射前和注射后第2天、5天、7天以及14天尾静脉采血检测血肌酐、尿素氮和CO2结合率(CO2CP),收集死亡大鼠及第7天和14天处死大鼠的肾组织进行病理检查。

Then transplantation of the sciatic nerve from donor rats to the recipient rats in two groups were progressed.7 days after nerve transplantation,the contents of cd4 and cd8 in peripheral plasma of recipient rats were detected by flow cytometry.8 weeks after nerve transplantation,transplanted nerve were observed under light microscope.results:compared with nonirradiated group,the percentage of cd4 and the ratio of cd4 to cd8 in uvb irradiated group were significantly decreased(p<0.05,p<0.01),cd8 percentage was significantly increased(p<0.01).conclusion:uvb-irradiated donor splenocytes were injected in the recipient rats through caudal veil,which could induce immunological tolerance to allogeneic transplantation.

未照射组注射未经紫外线照射的供体脾细胞,照射组注射经紫外线照射的脾细胞,7 d后移植供体神经。术后7 d行外周血t淋巴细胞亚群检查,术后8 w行移植神经组织学检查。结果:照射组cd4+百分率、cd4+/cd8+比值较非照射组明显降低(p.01,p.05),cd8+百分率较非照射组明显升高(p.01)。组织学检查显示,照射组的再生神经较非照射组多。结论:尾静脉内注射经紫外线照射的异基因脾细胞可诱导对异体神经移植的特异性免疫耐受。紫外线;脾细胞;免疫耐受;坐骨神经

MethodsSodium Hyaluronate was injected into the palatoglossal arch, palatopharyngeal arch and root of the tongue of eight Wistar rats. An electroencephalogram, electromyogram and oronasal airflow were recorded by PSG before and 4 weeks after injection when rats were under light anesthesia, which was expected to be similar to the sleep state.

方法将8只Wistar大鼠的双侧舌腭弓、咽腭弓及舌根处注射透明质酸钠,分别于注射前及注射后4周,以浅状态模拟睡眠状态,用多导睡眠仪监测动物的脑电、肌电及口鼻气流。

Mg/ kg way partially inhibited by L- NAME,or by sodium orthovanadate(50 ug/ kg),a potent inhibitor of protein tyrosine phosphatase;(2)genistein also decreased the PP of renal vascular bed in a dose-dependent manner and the effect of genistein was completely inhibiteby pretreatment with sodium orthovanadate,but unaffected by L- NAME;and(3 genistein decreased the PP of mesenteric vascula bed in a dose-dependent manner,an effect which was partially inhibited by sodium orthovanadate,but unaffected by L- NAME .

mg/ kg引起的效应;(2)向肾血管灌注环路中直接注射GST也可剂量依赖性地降低肾动脉的灌流压,预先注射正钒酸钠可完全抑制GST引起的效应,而L- NAME对此效应没有影响;(3)肠系膜血管灌流环路中注射GST可剂量依赖性地降低其灌流压,这一效应可被正钒酸钠部分抑制,而L- NAME对此无影响。

Four groups were respectively conducted in Morris water labyrinth test, which was used to dect the learning and memory of rats. Population spike in CA3 region was recorded in vivo following stimulation of lateral perforant path ,For the induction of LTP , 4 trains (50 shocks/ t rain , 500 Hz) high-frequency tetanic stimulations were applied to lateral perforant path.

MK-801组除按依赖组剂量注射海洛因外在每次注射海洛因前30分钟注射MK-801(0.3mg/kg),四组各自进行水迷宫实验测试大鼠学习记忆;在体记录由刺激海马外侧穿通纤维(lateral perforant path,LPP),所诱发的CA3区群体峰电位电位,并观察高频刺激引起的LTP效应;用逆转录聚合酶链反应技术半定量检测海马NMDA受体NR1、NR2A亚基mRNA的表达。

Other modes of therapy, however, such as subtenon抯, periocular, or intravitreal injections, are not as easily reversed if problems are encountered.

而另一些给药方式如球结膜下注射、球周注射或玻璃体内注射,当出现问题时就不那么容易逆转了。

Methods One injection was performed intra peritoneally with GAD purified from pig brain, together with Freund incom plete adjuvents, into female NOD mice at the age of 4 weeks as treated gro up (n=32), other 19 mice were injected FIA 100 μl alone as control group.

用猪脑GAD和不完全弗氏佐剂混合后给4周龄雌性NOD小鼠腹腔注射(32例),同时单独注射等量FIA作为对照组。8周龄时腹腔注射环磷酰胺以加速糖尿病。

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