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Cytolysosomes increased,cellular nucleus became twisted,rough endoplasmic reticulums swelled,and chondrosome became vacuolization.Dead intervertebral disk cells were located in ?nidi? which were made of multilayer degenerative collagen fibers.

术后8周实验组椎间盘开始出现大量死亡细胞,表现为溶酶体明显增多,细胞核扭曲,粗面内质网扩张,线粒体空泡化,死亡细胞外周的&巢&增厚呈多层排列,致密颗粒增多。

Results: Of all the semen samples, 8 were HCMV positive, 4 HSV-Ⅱ positive, but none were both HCMV and HSV-Ⅱ positive. HCMV late antigens were positively and HCMV early antigens negatively expressed in the spermatogenic cells of the 8 HCMV positive cases. In the 4 HSV-Ⅱ positive cases, 3 were positively and 1 weakly positively expressed. In the semen of the 12 positive cases were found large numbers of immature spermatogenic cells, with different manifestations of apoptosis, such as chromatin pycnosis, vacuoles, damaged nuclear membrane, and apoptotic bodies, but without virus infection-induced specific morphological alteration.

结果:83例精液样本PCR检测HCMV阳性8例,HSV-Ⅱ阳性4例,未发现2种病毒同时阳性病例。8例HCMV阳性病例ICC标记生精细胞HCMV晚期抗原均见阳性表达,HCMV早期抗原均为阴性表达;4例HSV-Ⅱ阳性病例ICC标记生精细胞3例阳性表达,1例弱阳性。12例病毒阳性病例均发现较多未成熟生精细胞,并有不同程度的核染色质固缩、出现空泡、核膜破损、核崩解、凋亡小体等凋亡现象,但未能找到病毒感染的特异性形态学改变。

Electron micrograph showed us that hepatocytes were damaged seriously along with the prolonged NHBD time in A group. At the NHBD 120 and 150min in A group, hepatocytes showed numerous cytoplasmic vacuoles and lysosomes, round swollen mitochondria with clear matrix and rough endoplasmic reticula which was not in a stacked arrangement.

电镜发现,A组随着心跳停搏时间的延长,肝细胞的损伤逐渐加重,至120和150min时,肝细胞内可出现大量空泡和溶酶体,线粒体和粗面内质网结构也遭到破坏,但未出现明显的坏死和凋亡改变。

Results: The cultured chondrocytes were polygonal cells. There were many rough endoplasmic reticula and mitochondria in cytoplasm, and a lot of secretory vesicles under cell membrane and in the cytoplasm. When cultured for 10 days, some small and white nodules were formed on the bottom of the culture dished, and volcanic-mouth-like structures were formed when cultured for 20 days. Both these nodules and structures contained GAG-positive substances were demonstrated by safranine-O staining.

结果:体外培养的软骨细胞为多角形,透射电镜下见胞浆内有丰富的粗面内质网系统及线粒体,胞膜下及胞浆中有较多分泌泡;连续培养10天时,培养瓶底部出现肉眼可见的白色结节,蕃红-O染色显示白色结节含大量氨基葡聚糖阳性物质,20天时形成火山口样结构,也被蕃红-O染成深红色。

Linear opacities appeared in pulmonary fibrosis of gradeⅡor gradeⅢand had statistical significance(P<0.05);Bullae and pneumothorax were mainly in pulmonary fibrosis of gradeⅢ,difference of appearance of the sign had statistical significance(P<0.05).The difference of appearance of ground-glass attenuation,consolidation,nodules and subpleural interstitial thickening had no statistical significance in different stages of pulmonary fibrosis. Honeycomb lung and traction bronchiectasis appeared in pulmonary fibrosis of gradeⅢ.

线状影出现于Ⅱ级和Ⅲ级PF,且具有统计学意义(P<0.05);肺大泡及气胸主要出现于Ⅲ级PF,与其余各级PF之间存在差异(P<0.05);磨玻璃影、实变、结节影及胸膜下间质增厚在各级PF出现的差异无统计学意义;蜂窝肺及牵引性支气管扩张都出现于Ⅲ级PF。

Linear opacities appeared in 14d group and 28d group,bullae and pneumothorax were mainly in 28d group,the difference of appearance of two signs above had statistical significance(P<0.05).The difference of appearance of ground-glass attenuation and consolidation had no statistical significance in sub-groups.Nodules and subpleural interstitial thickening did not appear in 3d group.

线状影出现于14d组及28d组,肺大泡和气胸主要见于28d组,上述两个征象出现的差异具有统计学意义(P<0.05);磨玻璃影和实变在各组中出现的差异无统计学意义;结节影和胸膜下间质增厚在3d组均未出现;蜂窝肺1只及牵引性支气管扩张2只都出现于28d组。3。

RESULTS: The passaged MSCs exhibited uniform appearance in fusiform shape, and their growth was slowed down after 9 passages, exhibiting cytoplasm vacuolization and body enlarging.

结果:传代培养的骨髓间充质干细胞形态均一,呈梭形、纺锤形,克隆样生长,培养至9代后生长减慢,并出现胞浆空泡化、胞体变大等老化现象。

Its smaller acini, the lack of Qu tubular, and normal morphology with complete loss, irregular stromal infiltration to collagen connective tissue layer no longer exists.

其腺泡较小,缺乏曲管状,正常排列形态完全丧失,不规则地向基质浸润,胶原结缔组织层已不存在。

According to the observed of TEM,mitochondrial of the epithelial cells were vacuolized, the cristae and membrane had disappeared. Rough endoplasmic reticulums were expansion.

透射电镜观察上皮细胞线粒体空泡化、嵴和膜消失,粗面内质网扩张,杯状细胞内含有大量的粘原颗粒。

Under the transmission election microscope, we had observed that the state of oocytes arid the cumulus cells around them was tightness, and there were plenty of long and thin microvilli playing the role of connection in the enwrapping. The shape of cumulus cells nucleus was ovate, and the longer and shorter diameters of the cumulus cells were 4.250±0.042μm and 2.750±0.07 μm respectively. The zona pellucida was well proportioned and tightly linking with oocyte. There were great deals of multi-crista mitochondria, lipid droplet and vacuolus. The mitochondria longer and shorter diameters were 0.600±0.106μm and 0.490±0.117μm. Granular nucleoplasma distributed very well in the cell nucleus.

电镜下,卵母细胞外的卵丘细胞紧紧包裹卵母细胞,且与卵母细胞之间有大量细长的微绒毛相联系;卵丘细胞核呈卵圆形,其长径为4250±0.042μm,短径为2.750±0.071μm;透明带均匀,与卵母细胞结合紧密;在皮质区集中分布大量的多峰型线粗体、脂滴和空泡,线拉体长径为0.600±0.106μm,短径为0.490±0.117μm;细胞核中颗粒状的核质分布非常均匀。

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