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It was noted that cell death from necrosis and autolysis would produce false-positive result because of non-specific DNA fragmentation.

提示类似泡状核细胞这类异染色质较少的细胞凋亡时可能都具有这种表现。

The operative area was examined with light microscope, electronic microscope, and the proliferating cell nuclear antigenwas detected. Results In CsA group, intraocular pressure was significantly lower than that in NS group(P<0.05), and more functional filtering blebs were observed. Less fibroplasia and proliferative cells, less bioblast and less active caryotin in fibrocyte were found in CsA group than that in NS group. No serious mcomplicaton was found in both group.

结果 环孢霉素A组眼压控制多较理想,与对照组间的差异有统计学意义(P<0.05),大多数形成功能性滤过泡,光镜下见极少量纤维组织增生,电镜下见纤维细胞线粒体少,染色质功能不活跃,增殖细胞阳性率低,与对照组差异有统计学意义(P<0.05),且没有浅前房、视网膜损害等严重并发症。

At 1st, 7th,14th,and 30th day after surgery, filtering blebs, intraocular pressure, cornea and ocular fundus were observed.the operative area was examined with light microscope, electronic microscope, and the proliferating cell nuclear antigenwas detected. results in csa group, intraocular pressure was significantly lower than that in ns group(p<0.05), and more functional filtering blebs were observed. less fibroplasia and proliferative cells, less bioblast and less active caryotin in fibrocyte were found in csa group than that in ns group. no serious mcomplicaton was found in both group.

结果 环孢霉素a组眼压控制多较理想,与对照组间的差异有统计学意义(p<0.05),大多数形成功能性滤过泡,光镜下见极少量纤维组织增生,电镜下见纤维细胞线粒体少,染色质功能不活跃,增殖细胞阳性率低,与对照组差异有统计学意义(p<0.05),且没有浅前房、视网膜损害等严重并发症。

TNF α protein and mRNA strongly expressed in the area of bronchiolar epithelium and the inflammatory cells in the lymph follicle near the bronchia and the alveolus cavity and pulmonary interstise in the model group.

此外,慢支大鼠TNFα蛋白及其mRNA在支气管周围的淋巴滤泡中的炎性细胞和肺泡间质浸润的炎性细胞中有强阳性表达,而经艾麻口服液治疗后,其表达减弱。

During the PCD, condensation of nucleus, marginalization of chromatin, nuclear membrane shrinkage and destruction apparently occurred, and many vesicles appeared in the cytoplasm, which may be related to the degradation of cell substances.

正在凋亡的细胞核染色质固缩、趋边、核膜破裂或皱缩、细胞质囊泡化并将降解的物质运到周围细胞,从而保证了其它细胞正常发育以及生长的细胞对物质和能量的需求。

Ultrastructure in the model group as compared with those in the control group was that mitochondrial swelling, unclear and vacuolar degeneration of crista mitochondriales, prosperity of endoplasmic reticulum, and apoptosis of hepatocytes and endothelial cells of hepatic sinusoid.

电子显微镜下可见,模型组大鼠肝细胞线粒体明显肿胀,嵴显示不清,部分呈空泡样变性,肝细胞内质网旺盛,可见肝细胞及肝窦内皮细胞凋亡。

There were three new discovers : 1. The phago-assistant membrane was a nonciliary membrane between two pieces of wave membrane. It tightened with Endoral membrane into cytoplasm formatting cytostome and cytopharynx; 2. Cytoplasm formatted digestive structure which is a stable network digestive channel system but casual food vacuoles. 3. The Oral Primordium field , the assemble and transport of microtubules and membrane were all in the aborve channel system.

有三项新的发现:吞噬辅助膜为位于两波动膜之间的非纤毛膜,并与口内膜一道包卷进入胞质形成胞口、胞咽;细胞质分化出固定的网络状消化腔隙而不是形成临时性的食物泡;口原基场及微管、小膜的装配运输都在此腔隙中进行。

The results of the TEM showed the same respiration epithelium in the inside gill and the gill filament, it is flat, the cytoplast of the epithelium contained of the rough endoplasmic reticulum, vacuole and mitochondrion in rich; the blood-gas barrier is formed of the monolayer epithelium, the endothelial cell and the basilar membrane between the both, following the development the blood-gas barrier attenuate gradually.

电镜结果表明花背蟾蜍外鳃与内鳃具有相同的呼吸上皮,为扁平状,上皮细胞质中富含粗面内质网及空泡和线粒体;气血屏障由由单层上皮细胞,内皮细胞以及二者之间的基底膜构成,随着个体的发育,气血屏障逐渐变薄。

After developing 48 hours, hepatocytes enters 2.0 mM t-butyl hydroperxide or 2.0 mM diamide to induced cell blebbing. The samples collected at 0, 15, 30, 60 minutes to analyze cell about membrane protein thiol deletion; the leakage of lactate dehydrodenase; the change of glutathione; the lipid peroxidation; the variation of actin and tubulin, and use Confocal fluorescent microscope to observe the change of actin and tubulin in rat hepatocytes.

肝细胞在48小时培养后,分别加入2.0mM t-buty1 hydroperoxide或2.0 mM diamide诱发细胞膜小泡生成,并分别於0、15、30、60分钟取样,分析细胞膜硫醇流失程度;乳酸去氢□渗漏;□胱甘□浓度变化;脂质过氧化;肌动蛋白与管蛋白变化;并利用雷射共轭焦萤光显微镜观察肝细胞中肌动蛋白及管蛋白的变化。

The strong positive expression located in the gland alveolus and duct of serous gland, as well as myoepithelium of mucus glands. In some cases, blood vessel endothelium and fibrous connective tissue also show weakly positive expression of NF-κB.

NF-κB的表达主要见于浆液腺腺泡上皮和导管上皮,呈强阳性表达;黏液腺肌上皮样上皮呈强表达;间质成分中血管内皮呈弱阳性表达、纤维结缔组织呈弱阳性表达。

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