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泡细胞

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We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果:体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;硒能增强重组VacA对胃癌细胞的抑制作用,使细胞分裂指数和集落形成率降低、S期细胞数量减少,G1期细胞总数增加;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

Ultrastructure in the model group as compared with those in the control group was that mitochondrial swelling, unclear and vacuolar degeneration of crista mitochondriales, prosperity of endoplasmic reticulum, and apoptosis of hepatocytes and endothelial cells of hepatic sinusoid.

电子显微镜下可见,模型组大鼠肝细胞线粒体明显肿胀,嵴显示不清,部分呈空泡样变性,肝细胞内质网旺盛,可见肝细胞及肝窦内皮细胞凋亡。

Numbers of secretory granules, oplasmic reticulums, mitochondrias in ACTH cells all changed distinctly. GTH cytoplast were full of big and small, high density electron secretory granules.STH cells were in activity synthesis and secretion, secretory granules were from fusion to release. TSH cells cytoplast evacuated secretory granules, electron density decreased and vacuofes appeared.

ACTH细胞分泌物颗粒数量、内质网及线粒体均发生明显变化;GTH细胞胞质中充满电子密度较高的大、小两种分泌颗粒;STH细胞处于活跃的合成和分泌状态,分泌颗粒呈现出由融合到释放的状态;TSH细胞胞质中分泌颗粒排空,且电子密度降低,出现大空泡。

Strain AR-15 penetrated the exodermal passage cells through the velamen cells or root hairs and further infected the cortical cells. There were a number of spores and hyphae in plant roots that was damaged by strain AR-15. Study on the ultrastructure of D. nobile root and its endomycorrhizal formationshowed that the wall of velamen cells of D. nobile root was thin, and there were waxylayers outside of velamen. Cells of exodermis were arranged tidily, having thick wallsand chloroplast.

金钗石斛根及其菌根的超微结构研究表明,金钗石斛根的根被细胞薄壁,在最外层根被细胞之外覆盖着蜡质层,外皮层细胞排列整齐,细胞壁厚,内含叶绿体,靠近外皮层的皮层细胞含叶绿体、线粒体,皮层细胞壁薄,细胞核明显,周围有叶绿体围绕,细胞膜附近有小泡结构。

The cortical cells had thin walls and mitochondrium, and nucleus wassurrounded by chloroplasts, and vesicles were next to cell plasmalemma. However, cellwalls of velamen, cortex and exodermis in the mycorrhiza of D. nobile had ring orpapillary thickenings in many cases. The hyphae were surrounded by moremitochondria and vesicles of plant, and the hyphae were digested and their structure waschanged at last. The cell wall of endodermis also had typical ring thickenings, and manysieve orifices in sieve tube elements of phloem were covered by callose, while therewere liaison cords in a few sieve orifices.

金钗石斛菌根的根被细胞的细胞壁多处出现环形圈,外皮层细胞壁加厚,木质化,也有环形圈存在,皮层细胞细胞壁多角形明显,加厚,皮层细胞中存在被消化、失去结构的真菌菌丝,在菌丝周围有较多的线粒体、小泡,内皮层细胞的细胞壁呈现的典型的加厚及木质化环形圈,韧皮部筛管的筛板结构,绝大多数筛孔已为胼胝质所覆盖,仅个别筛孔中还存在连络索。

Results: The main ultrastructural changes after Cd treatment included swelling or vacuolation of mitochondria, increase of myelin figures in GCT cells, and swelling of mitochondria in the endothelial cells of peritubular capillaries. The changes above were most prominent at 7~15d, and swelling of the cytoplasm and slight karyolysis were also found. The morphology of GCT cells didn't recovery to normal yet after 30d.

结果:镉注射后24h,GCT细胞内出现不同程度的线粒体肿胀和空泡化,胞质和线粒体内可见髓样结构,GCT周围毛细血管内皮细胞线粒体肿胀。7~15天后上述改变明显加重,部分GCT细胞见胞质广泛肿胀和轻度核溶解,30天后细胞形态仍未恢复正常。

Lipid droplets are intracellular organelles involved not only in lipid storage but also in cell signalling and the regulation of intracellular vesicular trafficking.

脂滴是细胞内细胞器官,它不仅参与脂质的存储还在细胞信号传导和细胞内小泡转运

The results indicated that there were only single mucosal mast cell sunset in rat mammary gland.They had various shapes.Most of mast cells were located in the interalveolar stroma and intralobular connective tissue.

结果 :AB- S染色显示,大鼠乳腺只存在黏膜型肥大细胞;无论是泌乳期还是静止期,乳腺肥大细胞大多分布于腺泡间和小叶间结缔组织中,细胞的形态各异,但细胞数量在静止期和泌乳期有显著差异,泌乳期的乳腺肥大细胞数量明显减少(P 。0 1)。

One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活,ICAM-1表达升高,与被激活的PMN表面表达的LFA-1相结合,&PMN-EC&相互作用加剧,一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍;另一方面粘附的PMN过度吞噬或激活,当白细胞吞噬的颗粒不能被封闭隔离,连同细胞内的酶被释放出来,其中的PMN-elastase能够降解细胞基质中各种成分,水解多种蛋白,加重胰腺的毛细血管内皮细胞和腺泡的损伤;释放的溶酶体酶使组织蛋白水解,产生的不饱和脂肪酸引发脂质过氧化方应,破坏细胞膜的结构和功能;释放的炎性细胞因子,激发其他的免疫细胞的功能,导致进一步的组织损伤和坏死,加重SAP的病理损伤,最终导致休克、脓毒血症及多器官功能障碍等严重后果。

Under the transmission election microscope, we had observed that the state of oocytes arid the cumulus cells around them was tightness, and there were plenty of long and thin microvilli playing the role of connection in the enwrapping. The shape of cumulus cells nucleus was ovate, and the longer and shorter diameters of the cumulus cells were 4.250±0.042μm and 2.750±0.07 μm respectively. The zona pellucida was well proportioned and tightly linking with oocyte. There were great deals of multi-crista mitochondria, lipid droplet and vacuolus. The mitochondria longer and shorter diameters were 0.600±0.106μm and 0.490±0.117μm. Granular nucleoplasma distributed very well in the cell nucleus.

电镜下,卵母细胞外的卵丘细胞紧紧包裹卵母细胞,且与卵母细胞之间有大量细长的微绒毛相联系;卵丘细胞核呈卵圆形,其长径为4250±0.042μm,短径为2.750±0.071μm;透明带均匀,与卵母细胞结合紧密;在皮质区集中分布大量的多峰型线粗体、脂滴和空泡,线拉体长径为0.600±0.106μm,短径为0.490±0.117μm;细胞核中颗粒状的核质分布非常均匀。

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