法位

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低，可能是由于其分子量较小，所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中，利用硫氧还蛋白融合方式对该抗原进行修饰，并在大肠杆菌中表达融合蛋白(Trx-B_2)，纯化该蛋白后免疫小鼠及家兔，结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后，得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度，并从免疫后的兔血清中提取IgG包被酶联板，利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清，结果阳性率分别为87.5%和43.75%，符合率为71.88%。

Susan, who was from Fargo, North Dakota, the daughter of a professional couple (an architect and a university professor), was waitressing at the time.

苏珊来自北卡罗来纳州的法戈，是教授家庭的女儿（她的父母一位是建筑师，位是大学教授）。

Good news for Farmar and the Lakers: He's looked just as explosive in the 2009-10 preseason as he did early last season, when his ability to push tempo off the bench was a key element to L.A.'s 20-3 start. However, a few games later, Farmar tore the lateral meniscus in his left knee and missed 17 games. Although getting back into rhythm was difficult for the backup point guard out of UCLA, his biggest contribution of the season came when Derek Fisher was suspended for Game 3 of the Western Conference Semis and Farmar contributed 12 points, seven assists, five rebounds and two steals with just one turnover in 33 minutes of L.A.'s 108-94 win.

法玛尔和湖人的好消息：在2009-10的季前赛他看起来好像大爆发一样，就像他上赛季做的一样，当他的能力在替补席上爆发出来的时候，他成为了湖人取得20胜3负开局的关键，在打完了一些比赛之后，法玛尔膝盖半月板韧带拉伤，他错过了休息了17场比赛，尽管随后他重回球馆，但是看起来他似乎对比赛还很困难，这位 UCLA 的组织后卫，对球队最大的一次贡献，是在西部半决赛第三场，当费舍尔被禁赛的时候，他顶替了费舍尔首发，在33分钟的上场时间里贡献了12分，7次助攻，5个篮板和2次抢断，帮助球队108-94赢下了比赛。

Methods The genotype of 4 SNPs in 108 simple CHD patients and 200 normal people were analyzed by restriction fragment length polymorphism and denaturing high-performance liquid chromatography. The statistical contingency table method was used to analyze SNP genotype frequency and gene frequency in patients and control group;then, the haplotypes were established and their frequencies in the two groups were assessed by PHASE software.

方法应用限制性片段长度多态性和变性高效液相色谱法结合测序，分析108例单纯性先天性心脏病患者及200名正常人4个SNP位点基因型；应用列联表法统计分析患者组和对照组各SNP位点基因型及等位基因频率；应用PHASE软件构建单倍型并统计分析患者组及对照组单倍型频率是否存在差异。

A phenomenally beautiful tenor voice like that, the rarest of boons, which Bloom appreciated at the very first note he got out, could easily, if properly handled by some recognised authority on voice production such as Barraclough and being able to read music into the bargain, command its own price where baritones were ten a penny and procure for its fortunate possessor in the near future an entr閑 into fashionable houses in the best residential quarters of financial magnates in a large way of business and titled people where with his university degree of B.

294他那男高音的音色极其纯美，表现出罕见的才华。布卢姆刚听了第一个音调就加以赞赏。倘若他能得到像巴勒克拉夫那样一位公认的发声法权威的适当指导，再学会读乐谱，既然男中音已多得烂了市，他就不难随意为自己标价。那样一来，不久的将来，这位幸福的美声歌唱家就有机会出入于经营大企业的财界巨头和有头衔者那坐落在最高级住宅区的时髦府邸。

Priests, scribes, and rulers, expounders and administrators of the law, were all brethren, children of one Father.

祭司、文士、官长，以及一切解释律法、执行律法的人，都是弟兄，都是同一位天父的儿女。

Shortly after their sale, Liu Yang, a Chinese lawyer who spearheaded efforts to have the pieces returned to Beijing, warned that the failure to return them would not soothe Franco-Chinese ties, frayed over the sensitive issue of Tibet.

拍卖刚结束，刘洋，一位高调诉讼要求将文物归还北京的中国律师，警告说，不能把铜首归还中国将不能缓解法中关系。法中关系因为敏感的西藏问题已经斑驳不堪。

This scaffolding has been placed in 3,465 patients from 2000 to 2005, Dr. Gobbi noted, and researchers have published studies on their results in about 450 patients. What is new about this study, Dr.

Gobbi医师指出，在2000年到2005年间，支架法已经运用在3465位患者身上，研究人员针对其中的450位患者发表过文章；但是，本研究有何新意？

The Baimiwong Gun Emplacement is located on the upland at the west bank of Keelung Port. It was probably installed more than 300 years ago during the Spanish and Holland colonial periods and is thus also known as the "Gun Emplacement of Fort Holland." It was once occupied by French troops during the Sino-French War. The Japanese colonial government remodeled the place to become the way it looks today.

白米瓮炮台位在基隆港口西岸高地上，与东岸的和平岛互为犄角，共扼基隆港门，形势非常险要，炮台可能早在 300多年前的西班牙及后来的荷兰占据时代就已设置，因此有「荷兰城炮台」之称，中法战争时法军曾占领过，今日之形貌为日治时代所改建而成的。

Linear regression method was used to analyze the correlation between facial height index and inclination of palatal plane, occlusal plane and mandibular plane in normal occlusion, and to establish floating norm diagram.

选取青少年正常头影侧位片158张及安氏Ⅱ类1分类错头影侧位片246张，利用线性回归法分析正常不同生长型的面高指数与腭平面、平面及下颌平面倾斜度的相关性，建立浮动正常值表，分析正常及安氏Ⅱ类1分类错不同生长型的上下颌骨的旋转类型。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。