法

Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法检测免疫血清抗体对ED的亲合力。2、对DNA疫苗组小鼠制备OVA致敏哮喘模型，检测气道压力峰值—时间指数、支气管／肺泡灌洗液中炎性细胞数量，PAS特染观察小支气管杯状细胞数量及RT-PCR法检测肺组织中粘蛋白MUC5AC mRNA水平，免疫组化和RT-PCR法检测GM-CSF的蛋白与mRNA水平。

Among the researches of his political thought, the following methods draw our attention: Burckhardt's analysis of overall humanity-culture; Skinner's research of historical context; Strauss' perspective of the mainstream traditional political culture.

学术界在对马基雅维里政治思想的研究方面，有如下研究方法值得我们注意：布克哈特所贯彻的人性－文化整体分析法；斯金纳所倡导的历史语境探究法；斯特劳斯所主张的主流传统政治文化透视法。

As a contrast, people can adopt a criterion of nature of humanity for burgher in real law that has a function to distribute scarce resources in a society.

作者认为，在民法典中，不宜采用统一的人性标准，在起社会组织功能的人法中，可采用公民的人性标准；在起稀缺资源分配法功能的物法中，则可采取市民的人性标准。

Burnside had sent several army officers to listen to the speech. When they reported what Vallandigham said, Burnside ordered his arrest. Without question, the man had violated the General's orders.

伯恩赛德派了几位军官去听法兰迪加姆的演讲，当这些军官报告法兰迪加姆所说的内容时，伯恩赛德命令将他逮捕，毫无疑问，法兰迪加姆违犯了这位将军的命令。

With the use of karst water deposit (calc-sinter) analysis system, historical document analysis system, and modern observation data statistic analysis system, we study the evolutive regularity in three temporal scales and the evolutive process in geologic temporal scale in Niangziguan karst water spring, and divide this karst water evolution into five stages.

同时运用岩溶沉积物分析法、历史文献分析法、现代观测资料的统计分析法研究了娘子关泉岩溶水系统在三个时间尺度上的演化规律及在地质时间尺度上的演化过程，并将该岩溶水系统演化分为五个阶段。

In this paper, from two aspects of the total potential method and abnormal potential, three types of boundary conditions which affect calculating results in the finite element method are analyzed, and this provides a foundation for using the appropriate boundary conditions and selecting the best distance to enhance its accuracy.

本文从总电位法和异常电位法两个方面，分别就直流电法有限元中所采用的三类边界条件对计算结果的影响进行了分析，为采用合适的边界条件及选取最佳边界距离来提高正演精度提供了基础。

In its relation to the secular state, Islamic law differed from both Jewish and canon law.

在其与世俗国家的关系上，伊斯兰法亦有别于犹太教法和教规法。

The intralellular labeling methods developed for single cell analysis are summarized in this review, including cell itself acting as a microreaction chamber for derivatization, dericatization mediated by liposome or polyethylene glycol to increase permeabilization of cell membrane, on- and post-column derivatization during single cell analysis with capollary electrophoresis or microchip electrophoresis and labelling of cells with quantum dots.

本文综述了在单细胞分析中常用的荧光标记方法，包括细胞作为微反应器的衍生法，借助于脂质体与聚乙二醇等增加细胞膜通透性的衍生方法和在毛细管/芯片毛细管电泳分析单细胞时柱上衍生和柱后衍生法以及量子点的标记法等。

Research and application of treating waste water with carbinol by biochemical method ;2. Biochemical method and Fe-C method were used for the treatment of terephthalic acid wastewater.

采用生化法和铁碳法进行对苯二甲酸废水的处理研究，结果得出：CODCr=2000mg/L是微生物降解对苯二甲酸的抑制质量-体积分数；pH值为8～9时活性污泥处理对苯二甲酸废水比较理想；铁碳法处理对苯二甲酸废水35h后，CODCr的去除率达到了80%以上。

The expressions of HO-1 and HO-2 mRNA were detected using reverse transcription polymerase chain and the relative amount of CO released into the media was quantitated as carboxyhemoglobin by enzyme linked immunosorbent assay.

通过逆转录聚合酶链式反应和免疫细胞化学法检测HO-1、HO-2 mRNA和蛋白的表达，酶联免疫法检测培养上清液中碳氧血红蛋白含量，四唑氮比色法检测细胞增殖。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。