法

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

The main contents are as follows: In chapter 1, systematic conclusions of intension and characteristic of sustainabledevelopment of real estate industry are made after the review of home and foreign research achievements; Chapter 2 analyzes status quo and trait of Chinese real estate industry in 2005, from views of investment, exploitation and sale, three parts of real estate industry, with the help of massive graphs, as well as elaborates main problems existing in real estate market; In chapter 3, the gambling thought is used to divide the developing process of Chinese real estate market into three phases, according to the change of main market participators and their behaviors; Finally, in chapter 4 and 5, a system with 72 indices in research on sustainable development of real estate industry in china is established, and theories of Catastrophe, Cosines Vector Included Angle, Entropy and CRITIC are applied to appraise the current situation of sustainable development of real estate industry in 31 provinces and cities in 2004, then the consistency test of appraisal results is carried out by Kendalls Coefficient of Concordance and Spearman Correlation Coefficient.

本文的主要内容如下：第一章在查阅大量国内外有关可持续发展理论、房地产业有关研究文献的基础上，评介了有关理论概念，并对房地产业可持续发展的内涵进行归纳、总结；第二章从房地产投资、开发和销售三个环节入手，结合大量图表，分析2005年中国房地产业的发展状况和特点，揭示我国房地产市场存在的主要问题；第三章利用博弈思想，根据市场参与主体及其行为的变化，将中国房地产市场的发展过程分为三个阶段；第四、五章构建了一个由72个指标构成的房地业可持续发展指标体系，利用突变评判法、向量夹角余弦法、熵值法和CRITIC法对2004年全国31个省市的房地产业可持续发展状况进行评价，并运用肯德尔和谐系数和斯皮尔曼等级相关系数对评价结果进行一致性检验。

Electrochemical behaviors of chlorphenamine maleate at carbon paste electrode and its electrochemical kinetic parameters have been investigated by cyclic voltammetry, bulk electrolysis with coulometry, chronoamperometry and chronocoulometry.

运用循环伏安法、控制电位电解库仑法、计时电流法和计时电量法研究了马来酸氯苯那敏在碳糊电极上的电化学及其电化学动力学性质。

Their direct electrooxidative behaviors and the parameters of electrochemical kinetics were obtained by cyclic voltammetry,bulk electrolysis with coulometry,chronocoulometry,chronoamperometry.

运用循环伏安法考察了苦参碱和槐定碱的直接电化学氧化行为，用控制电位电解库仑法、计时库仑法、计时电流法测定并计算了它们的电化学动力学参数。

Genetic variation in demes appeared 8 times higher than among demes. The paper also gave a few methods such as dendrogram, statistic, frequency of markers, polymorphic bands and special loci, genetic variations within and among demes for conservation and utility, molecular checking indexes for cultivars can provide basic information for protection of intellectual property rights and selection of wanted cultivars.

通过多态带和特殊带提出了桃核心种质，并利用聚类法、统计法、标记频率法、多态带数和特殊位点法、类群间和类群内的遗传差异法提出了种质保存策略；以及品种识别分子检索表的建立，为知识产权保护和正确选择品种提供了第一手资料。

Lines/mm holographic gratings in ultra low expansion mold plate were replicated onto the cross section of the measured sections in a ball grid array electronic package and a quad flat package.

应用1200线/毫米的云纹光栅并结合云纹干涉法，电子束云纹法，原子力云纹法，扫描电镜云纹法测量QFP和BGA型电子封装组件的热变形。

In order to find out a simple and efficient method to calculate the water breakthrough time in combination well pattern of horizontal and vertical wells, simplifing a horizontal well into a vertical well with the same well-hole radius, and considering the unpiston-like displacement, the water breakthrough time formulas of five-spot, seven-spot and nine-spot pattern were derived and revised by equivalent percolating resistance method. In this method, the percolation region is divided into three resistance regions.

为了寻求一种简单、有效的计算水平注水井与直井联合井网的见水时间的方法，将水平井简化为具有等效井筒半径的直井，考虑水平井与直井联合布井水驱油的非活塞性，运用等值渗流阻力法将渗流区划分为3个阻力区，推导出了五点法、七点法和九点法水平井与直井联合井网的油井见水时间的计算公式和修正公式。

Monitored on the spot, the deformation characters of these supporting constructions are studied. Campared the calculation translocatins with the monitored translocations, the thesis believes that the translocation can be calculated by the method of "m", when the supporting structures produce small information. The translocation can be calculated by the mothod of "rigid frusta" or "the experiential expressions of shanghai area", when the supporting structures such as cement-soil wall in soft soil area produce big information.

通过实测，研究了小直径双排桩、水泥土深搅桩、土钉墙、放坡开挖等支护类型的变形特性，并用实测位移值与不同位移计算方法得出的结果进行了比较，认为对变形较小的支护类型可以采用& m&法计算位移，而对于软土基坑中变形较大的支护结构重力墙不适宜采用&m&法，其计算可以采用和参照&刚性墩&法和&上海地区经验公式&法。

After analyzing the current conditions of English grammar teaching and comparing the methods of grammar translation with communicative approach, this paper sets forth the applications of communicative approach in grammar teaching to improve the grammar of college English learners.

交际法在语法教学中的运用，革除了枯燥无味讲语法的旧习。本文针对语法教学的现状，通过传统的语法-翻译法与交际法的对比，论述交际法的理论体系，提出运用模拟情境、角色扮演、小组讨论等交际教学法进行语法教学，以提高大学生对英语语法的掌握。

It introduces the cross correlation speed measurement, gravity-fed flow experiment device and the gas-solid flow laboratory equipment, to do systemic experimental research on the characters of the gas-solid flow measurement.

分别采用了相关速度测量法、自由落体实验平台法、气固两相流综合实验平台法，对气固两相流的静电法测量特性作了系统的实验性研究。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。