法

There are three types of transplantation: sods, plugs and shoots with rhizomes.

移植法是目前最常用的方法，可分为草皮法、草块法和根状茎法。

Aiming at the characters of the assessment, four means of landscape impact prediction are introduced: perspective map, superimposing images, making models, and showing with computer.

针对景观影响评价的特点，介绍了透视图法、叠图法、制作模型法和计算机显像法4种景观影响预测的手段。

METHODS:Based on the extraction rate of chlorophyll,acetone method was compared with alcohol method and the preparing factors of acetone method was optimized by orthogonal design.

以叶绿素提取率为指标，对脱镁叶绿酸制备的乙醇法和丙酮法工艺进行比较，用正交试验法对丙酮法的工艺条件进行优化。

In the paper, the BP Neural Network is introduced to pick up the first break. And aiming at the shortcomings of BP neural network, such as the low speed in training, the tendency to local minimum, the limitation of amplitude, the momentum factor method and the self-adjusting learning rate are used to improve the BP algorithm.

将BP神经网络引入到初至波拾取中，并针对经典BP神经网络收敛速度慢、易于陷入局部极小的缺点，利用组合函数法、限幅法、动量因子法及自适应学习率法等进行了改进。

The data source based on the second soil survey in year of 1982 and repeated soil sampling in year of 2003, and the methods for sampling, determining and analyzing of SOC are auger hole method, K_2Cr_2O_7 oxidation method, area-weigh method respectively, and micro-pipette method is used to determine soil texture....

SOC数据来源于1982年土壤普查和2003年重复采样，土样的采集、分析和统计方法分别为土钻取土法、重铬酸钾氧化法、吸管法和面积加权法。

Based on fuzzy mathematics comprehensive evaluation, the toxicity of heavy metals was taken into account. The fuzzy comprehensive evaluation results by pollutant concentration exceeding standard weighting method, toxic response coefficient heck-stepping index weighting method and heavy metal toxicity sorting weighting method were compared with the potential ecological risk evaluation results. The research results showed that the evaluation results by toxic response coefficient back-stepping index weighting method could reflect the actual results more truly and accurately.

在模糊数学综合评价基础上，将重金属的毒性纳入权重考虑，通过污染物浓度超标赋权法、毒性响应系数反推指数赋权法和重金属毒性大小排序赋权法的模糊综合评价结果与潜在生态危害指数评价结果比较，研究表明毒性响应系数反推指数赋权法的评价结果更能真实准确地反映实际结果。

It effectively solves the problem of contact mechanics between burden granules. The dynamic data structure that is a linked list is built to save and delete the varying data in simulation calculation process. Therefore, the distinct element method simulation model of charging bell-less top blast furnace is established in view of the distinct element method model of blast furnace burden granular.

本文首先对离散单元法的基本原理和数值计算方法进行了系统研究，针对高炉炉料颗粒介质特点，对离散单元法进行了相应改进，建立了高炉炉料颗粒运动的离散单元法基本数学模型，推导出了相应的高炉炉料颗粒离散单元法数值计算基本参数的计算公式，例如阻尼、刚度、时步等，并确定了合理的数值模拟参数值。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Many kinds of treatment are used for fluoride removal. Conventional methods including coagulant sedimentation, active alumina adsorption, bone char adsorption are not suitable for rural areas owing to operation and management. Membrane methods for defluoridation, such as electric infiltration and reverse osmosis, have the advantage of convenient application, simple operation and good adaptability.

用于饮用水除氟的水处理技术有多种，常规除氟技术的有混凝沉淀法、活性氧化铝吸附法、骨炭吸附法，常规除氟技术由于受技术操作、管理、运行等要求影响，不宜在农村使用，膜法除氟技术，如电渗析、反渗透等应用方便，操作简单，适宜性强。

In this paper, the status quo of the Risk Management of the domestic and foreign research as well as applications of our city Yikatong projects was reviewed. The basic concepts and several commonly used methods of risk indentify, risk evaluation and risk control, were introduced. And present situation of the project management research was summarized. Which is mainly: The methods of risk indentify: Brain Storm, Delphi and Situation Analysis; The method of risk evaluation: Fuzzy-Synthesis

本文对项目风险管理在国内外的研究现状以及在我国城市一卡通项目中的应用进行了综述，介绍了它的基本概念及其常用的几种风险识别、风险评价和风险应对和监控的方法，总结了城市一卡通项目中风险管理的研究现状，主要为：风险识别的方法：头脑风暴法、德尔菲法和情景分析法；风险评价的方法：模糊综合评价法。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。