法

In this paper, we studied the separative effect of protein components in crude Morinda polysaccharide by the Sevag method in order to compareison through Kjeldahl Determination of protein content change before and after separation, and to anthrone-sulfuric acid measured the content changes polysaccharide content before and after separation.

本文通过以凯氏定氮法测定巴戟天粗多糖经Sevag法分离前后蛋白质含量的变化，并以蒽酮一硫酸法测定分离前后多糖含量的变化比较，研究了用Sevag法对巴戟天粗多糖中的蛋白质成分分离的效果。

According to the theory of the coagulation method it can be classified two types, one is the measure with photoelectric and nephelometric analysis; the other is the measure with double coil and a magnetic steel ball. coagulation method is physical quantity changes below coagulant acting on blood plasma, for example light、electricity、machine movement etc, and then the data is analyzed by computer to final result, so coagulation method is also called biology-physics method. Photics'blood coagulation apparatus determines coagulation by nephelometric'changes in the coagulate process.

凝固法是通过检测血浆在凝血激活剂作用下的一系列物理量的变化，由计算机分析所得数据并将之换算成最终结果，所以也可将其称作生物物理法；光学式血凝仪是根据凝固过程中浊度的变化来测定凝血的；双磁路磁珠法其原理是利用血浆凝固过程中，在其内部运动的物体所受阻力增加，因此通过测定受阻情况可以反映血浆凝固情况；另外近些年还有很多人采用压电石英晶体法来进行血凝研究。

Methods: Forty mice were divided into four groups randomly:normal group,model group,ligation group and treatment group.The model was made by the method of partly ligating the recta outside the body.The level of endotoxin in blood plasma was measured by dynamic nephelometric method and the content of TNF-a in blood serum was detected by the method of radioimmunity.(2)After the mice were killed,their left lungs were taken out for bronchi-alveoli fluid, then the content of MDA in BALF was tested by TBA method.(3)The vitality of PAM in BALF was countied on the counting set of erythrocyte,and the blue-dyed cellswere counted, then the percentage of PMN was worked out.(4)The pathological features of the mice\'s lung tissue were observed with routine and ultramicro pathological mechanism.

①将40只大鼠随机等分为正常组、模型组、解扎组、治疗组，以体外直肠不全结扎法造模，以动态浊度法检测各组大鼠血浆内毒素水平，放射免疫分析法检测各组血清TNF-α含量；②大鼠处死后，取左肺进行支气管肺泡灌洗，以TBA法测定支气管肺泡灌洗液中丙二醛含量、白蛋白含量及肺表面活性物质水平；③在红细胞计数盘上作BALF中肺泡巨噬细胞活力计数，计数兰染的细胞数，求出死亡百分率；在光镜下计数BALF沉渣200个细胞，计算中性粒细胞所占百分比。

METHODS:Nestin expression was detected in 33 cases of neuroepithelial tumors using the immunocytochemical staining. The Nestin positive and negative cells were isolated by magnetic activated cell sorting. Nestin positive cells were culutered, identified, and induced to differentiate. The expression of Nestin protein was detected in Nestin+ cells by RTPCR.

采用免疫组化法检测神经上皮肿瘤组织中Nestin的表达情况，免疫磁珠法分选肿瘤组织中的Nestin+细胞，悬浮细胞培养法进行Nestin+细胞的增殖、分化与鉴定，RTPCR法检测Nestin蛋白在其中的活性表达情况。

The results calculated by the Elovich rate equation showed that K release rates from 1 mol.L-1 HNO3 extraction ＞those from 2 mol.L-1 HNO3 extraction ＞those from H-resin extraction. Elovich equations combining with the data of H-resin and 2 mol.L-1 HNO3 successive extraction could well described the release characteristics of soil K. The correlation coefficients of the equations reached most significant levels (r＝0.956**—0.999**), and standard errors of specific K release capacities between the vaules calculated by the equations and those measured by both successive extractions were smaller(s=1.61 and 3.52). The specific release rates of soil K calculated by the Elovich rate equations were significantly correlated with the indices of soil K availability (the amounts of easily available K, nonexchangeable K, total available K in soils and K absorbed by ryegrass).

研究结果表明：1 mol.L-1 HNO3提取法的释K速率＞2 mol.L-1 HNO3提取法的释K速率＞氢饱和交换树脂提取法的释K速率；氢质交换树脂和2 mol.L-1 HNO3提取法结合Elovich模型较理想地描述了土壤K素的释放特性，拟合方程的相关系数均达极显著水平(r＝0.956**-0.999**)，土壤特征累积释K量的计算值与实测值的标准差较小(s=1.61和3.52)，且通过速率方程计算的土壤特征释K速率与K素有效性指标(速效K、缓效 K、有效K及黑麦草总吸K量)的相关性也较为密切。

The genomic DNA of Nosema bombycis was isolated from it's spores, using two methods, ie, Alkaline-Germination and Liquid-nitrogen.

分别用碱发芽法及液氮法抽提了家蚕微孢子虫基因组DNA，其中碱发芽法抽提的DNA质量及数量上好于液氮法抽提的DNA。

To investigate the relationship between the impulse oscillometry method and plethysmogrophy used in measuring airway resistance,Using the two methods to measure the airway resistance and the ventilation data of 158 patients.

为探讨脉冲振荡法和体描法测定呼吸道阻力的关系，对158例患者分别用脉冲振荡法和体描法测定呼吸道阻力，同时测定测试患者的通气功能，对2种方法测得的呼吸道阻力进行相关分析，考察各组数据的分布情况。

The non-isothermal crystallization kinetics of polyoxymethyleneand poly- oxymethylene/co-polyamideat different cooling rates were investigated uisng differential scanning calorimetry.

采用差示扫描量热法研究了聚甲醛和聚甲醛/盐聚酰胺二元共混体系在不同降温速率下的非等温结晶行为，并用 Jeziorny 法、Ozawa 法和莫志深法计算了聚甲醛及其共混物的非等温结晶动力学参数。

Methods Freshmen and junior of medicos were selected,and parasitological examination were conducted for the infections of intestinal parasite,in-cluding physiological saline direct smear method,saturated brine flotation,modified Kato's thick smear and natural sedimentation.

目的 了解某高校医学生肠道寄生虫感染状况，在校学生寄生虫防治工作提供依据方法采用生理盐水直接涂片法、饱和盐水漂浮法、厚涂片透明法、自然沉淀法对某高校、三年级大学生659人进行调查。

Methods Freshmen and junior of medicos were selected,and parasitological examination were conducted for the infections of intestinal parasite,in-cluding physiological saline direct smear method,saturated brine flotation,modified Kato's thick smear and natural sedimentation.

采用生理盐水直接涂片法、饱和盐水漂浮法、厚涂片透明法、自然沉淀法对某高校一、三年级大学生659人进行调查。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。