法

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

It is a organic hexahydric weak acid. In different acidity, the dissociation degree and the color of reagent solvent were different also. Determined and draw the absorption curve of reagent in varies acidity, and figured out the dissociation constant by Perisic-Janjic method; the complex ratio of the reagent and bismuth was determinated by mole ratio method and continuous variation method; The apparent stability constant of the complex was calculated, the mechanism of dissociation and coordination were discussed.

2，6—二溴4—硝基偶氮氟胂是本实验室合成的测定铋的一个较好的试剂，对其进行了进一步的研究，在用柱层析法对其进行了提纯后，测定了试剂在不同酸华东师范大学硕：l：学位论文摘要度下的吸光度和最大吸收波长，绘制了其吸收曲线，用Perisic一Janjic法计算了试剂的六级离解常数；用摩尔比法和连续变化法测定了试剂与秘形成的配合物的络合比：计算了配合物的表观稳定常数，研究了其离解和配合机理，为筛选高效有机显色剂提供了理论依据。

The direct calculation of the GaN/AlN super cell demonstrated that the GaN/AlN heterojunction was homotype and changed abruptly at the interface.

另外，通过使用常用的平均键能法、平均势法和芯态法三种近似方法对GaN/AlN带阶的计算，比较得出，超原胞法虽然计算量较大，但能够给出异质结界面附近更为详细的信息，这一点其他三种近似方法无法得到，但他们也能够得出与实验值基本一致的带阶参数。

For instance, the organic links among the use of aroma-therapy products including massage, SPA hydropathy, fumigation inhalation, oral lavage method, spraying methods, and the 'certain range' of products used in those methods such as aromatic oil, plant media oil, Hydrosol, plant roots, stems, leaves and fruits.

例如芳香疗法中所使用的产品在使用方法上包括按摩法、水疗法、薰蒸吸入法、内服灌洗法、喷洒法，这些方法所使用的&一定范围&产品例如芳香植物油、植物媒介油、纯露、植物的根、茎、叶、花、果实所制取的干制产品在各项标准之间又具有内在的&有机联系&。

Methods:Animal models of 25 adult mixed breed dogs were established by transverse osteotomy at bilateral iliopectineal crest,which were fixed with ATMFS anterior column connector and steel plate respectively. The animals were examined at 2,4,6,8,and 12 weeks by X-ray,macropathological observation and biomechanical test. Tetracin fluorescent labeling,HE staining,Masson triad colour staining,in situ hybridization and immunohistochemical method were performed and histological image analysis were applied.

建立犬双侧骨盆弓状线骨折的动物模型，分别采用ATMFS前柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周分别行影像学检查、大体观察及生物力学测试；采用四环素荧光标记法、HE染色、Masson三色染色法、原位杂交法、免疫组织化学法进行组织学观察并图像分析；采用RT-PCR法检测骨折端不同时期骨钙蛋白和核心转录因子mRNA相对表达量的变化。

Based on the real observation data, forecast equitation or forecast model, the dynamics method such as divergence and vorticity calculation, real data correction, ascending and subsidence calculation was used to simulate the 4D field of divergence, vorticity and vertical velocity at different time, different range and different levels. The field was connected to 3D fields of insect trajectories, yielding the flying speed, direction, ascending area and subsidence. Thus the peak of immigratory insect could be timely monitored. A system of the peak of immigratory insect forecast was set up.

根据实际观测资料、数值预报方程或数值预报模式等输出的风场网格化处理结果，利用运动学方法、散度推算法、涡度推算法、实际资料订正法、升降法等对不同时间、不同范围、不同层次的四维散度、涡度和垂直运动速度场进行计算或模拟，将计算或模拟得到的散度场、涡度场、垂直运动速度场与昆虫迁飞的三维气流场联系起来，得到该类昆虫所具有的迁飞速度、方向、上升区和下降区，从而对昆虫的迁入峰进行实时预警。

The parade asks Legislative Yuan to pass "No Gender Discrimination Law" and "Cohabitation Law" in order to eliminate discriminations and institutional challenges that LGBT community faces in school, marriage and job market. In addition, the government treats sex workers inhumanely by using Social Order Maintenance Act and Articale 235 of Penal Code. Police sets up traps to arrest sex workers and maliciously sweeps LGBT gathering places.

今天的游行提出订定「反性别歧视法」、「同居伴侣法」等诉求，主要针对LGBT社群在就业、婚姻、求学过程中遭到的歧视与制度上的阻碍；除此之外，国家机关透过社会秩序维护法、刑法235条等恶法，与警察机关利用「钓鱼」等方式对「援交」、「性工作者」采取不人道的对待；对於LGBT社群聚集的场所，恶意地扫荡、破坏社群的生存权利。

Both of the algorithms with two forms for the parallel data and fan beam data respectively are developed based on the interpolative algebraic reconstruction techniques.

这两个算法对于不同的相叠采样模式，具体表现为四个算法：平行束的虚拟探测器法、平行束的直接重建法、扇形束的虚拟探测器法和扇形束的直接重建法。

Several routes for synthesizing methyl isopropyl ketone were introduced, including the main methods based on isobutyl aldehyde-acetic acid, isobutyric acid -acetic acid, methyl ethyl ketone-formaldehyde and isoprene.

介绍了甲基异丙酮的多种合成路线，主要有异丁醛-乙酸法、异丁酸-乙酸法、甲基乙基酮-甲醛法和异戊二烯法。

Design of strength calibration application program for pump shaft parts;2. The calibration of parameters is a key step in the application of IWA Activated Sludge Models.

对国际水协的活性污泥法动力学模型在应用中遇到的参数校核问题进行研究，总结了目前常用的三种方法：专家法、灵敏度分析法和过程工程法。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。