法

The paper introduces the construction and support situation of the substep drivage method at the drivage face under the big thickness change etc.

介绍了祁东煤矿在煤层厚度变化大等复杂条件下的掘进工作面应用分台阶掘进法施工及支护情况，包括应用背景、支护参数确定、分台阶掘进法简介、施工工艺及实验手段、分台阶掘进法使用后对工程质量采取的措施、分台阶掘进法使用前后比较、应用比较，应用效果等。

It includes: the application background,the determination of support parameters,the synopsis of substep drivage method,the construction craft and experimental method,the adopting measures to the project quality after using the substep drivage method,the frond and back application of the supstep drivage method,the useful effect etc.

介绍了祁东煤矿在煤层厚度变化大等复杂条件下的掘进工作面应用分台阶掘进法施工及支护情况，包括应用背景、支护参数确定、分台阶掘进法简介、施工工艺及实验手段、分台阶掘进法使用后对工程质量采取的措施、分台阶掘进法使用前后比较、应用比较，应用效果等。

Moreover, adsorptive property of compound on electrode was studied by some methods, such as linear sweep voltammetry , normal pulse voltammetry , electrocapillary measurement , cyclic voltammetry.

此外，还利用线性扫描伏安法、常规脉冲伏安法、电毛细管曲线法、循环伏安法等方法研究了体系物质在电极上的吸附性质。

In this paper, the experience method, gas phase pseudopressure method , B-W method, oil reservoir integration method and approximation method for analysing gas condensate well testing are emphatical- ly described, and their characters, correctness and application scope are also compared.

着重介绍经验修正法、气相拟压力法、B-W方法、油藏积分法和近似法等，并比较了它们各自的特点、正确性及适用范围。

This research conducts appraisal system of bodily shape and the physical quality and each appraisal index and the standard which has been connected in primary selection of eurythmic gymnastics in our country through the literature material method, the expert interview method, the questionnaire survey method, measured method and mathematical statistic.

本研究通过文献资料法、专家访谈法、问卷调查法、测试法和数理统计等方法对我国艺术体操初级选材的身体形态和身体素质评价体系进行研究，并构建了各相关评价指标及标准。

It made a summarize about the basic condition, final aims, basic regulations and limitations of WTO and those of Convention on International Trade in Endangered Species of Wild Fauna and Flora. Based on that, a analysis was made for the possible conflicts between WTO and CITES through the comparison of some typical cases about wildlife trade. It suggests that the regulations and policies associated with environment and trades, the regulation of "the latter excelling the former" and "the especial excelling the normal", and the "exceptive" regulation can be used to unpuzzle and avoid the conflicts.

将WTO及CITES的基本情况、最终目的、基本原则及局限性作以概述，并在此基础上，通过对国际上野生动植物贸易的典型案例的比较对WTO与CITES规则之间可能存在的冲突作出了剖析，提出可利用环境与贸易的有关原则和政策、&后法优于前法&和&特别法优于一般法&原则以及&一般例外&原则对这些冲突进行解释和规避。

METHODS: Cytometry and soft agar were employed in observing the biological characters of MEC-1 and Mc3 cell strain. The expressions of the two sub-types of MEC-1 and Mc3 cell strain, SSTR1 and SSTR2, were detected by hybridization in situ. The condition of the binding between MEC-1, Mc3 and 125I-RC-160 was analyzed by radioactive genin binding analysis.

采用细胞计数法、软琼脂法等观察MEC-1及Mc3细胞株生物学特性；以原位杂交法检测MEC-1，Mc3细胞株SSTR1及SSTR2两种亚型的表达情况；以放射配基结合分析法分析MEC-1，Mc3细胞与125I-RC-60的结合情况。

METHODS AK was purified by DEAE ion-exchange cellulose(DEAE-52) and sepharose Cl-4B .The component of the saccharide was determined by GC and TLC.Its MW was measure d by gel filtration.The form of glucosidic bond was detrermined by IR.The conten t of the saccharide was measured by colorimetry.

采用DEAE纤维素(DEAE-52)柱层析和凝胶柱层析分离纯化草乌多糖，气相色谱法和薄层层析法测定其多糖组成，凝胶过滤法测定其相对分子质量，红外光谱测定其苷键类型，比色法测定其糖含量。

Phaeocystis cells are separated experimentally form the water samples collected in Bohai Phaeocystis blooms in 2005.Plate and dilution was used to separate and culture the Phaeocystis cells from the Bohai water samples; Its ecological properties of growth was studied in this research; The break effect of ultrasonic and freezing method was compared in the breaking treatment of Gymnodinium spp. cells of different periods.

该研究选用平板法和稀释法对渤海棕囊藻赤潮期间水样中的微藻进行了分离培养实验；系统研究了裸甲藻在实验室条件下的生态学特征；考察了超声波法和冻融法对不同生长时期裸甲藻细胞的破碎效果；运用高效液相色谱对裸甲藻和链状亚历山大藻细胞提取物进行了麻痹性贝毒的分析检测。

In this study, different nano-hydroxyapatite particles,HAP_1(25-60nm), HAP_4(the additives is heparin, 15-50nm), HAP_5(the additives is bovine serum albumin BSA, 20-80nm) were prepared by homogeneous precipitation and used heavy-gauge hydroxyapatite as comparison,we determined amount of heparin, sialic acid ,BSA adsorbed on HAP_1,HAP_2,HAP_3 by Crystal Violet assay, Bialsche method,Bradford method respectively and analyzed the binding mechanism by infrared spectrum;After taking HAP_1,HAP_2,HAP_4,HAP_5 and RBC to co-culture in vitro,we studied RBC hemolysis test and detected RBC hematolysis rate by erythrocyte osmotic fragility test;observing the changes of morphous and locomotion of cell after coacting HAP_1,HAP_2,HAP_4,HAP_5 and RBC by light microscope and inverted phase contrast microscope;observing HAP_1,HAP_2,HAP_4,HAP_5 effecting on Ultrastructure of RBC.

本文用均匀沉淀法制备了HAP_1(25-60nm)，HAP_4(添加剂肝素，15-50nm)，HAP_5(添加剂牛血清白蛋白BSA，20-80nm)等纳米粒子，并用大尺寸的羟基磷灰石HAP_2(470-520nm)，HAP_3(1906nm)作对照，分别利用结晶紫法、Bialsche法、Bradford法研究了肝素、唾液酸、血清白蛋白在HAP_1，HAP_2，HAP_3上的吸附量，用红外光谱分析其中的结合机理；在体外将HAP_1，HAP_2，HAP4_，HAP_5与红细胞共培养，进行了红细胞溶血试验的研究，并借助红细胞渗透脆性试验检测红细胞溶血率；运用普通光学显微镜和倒置相差显微镜观察了HAP_1，HAP_2，HAP_4，HAP_5与红细胞作用后细胞形态及运动的变化：透射电镜观察了HAP_1，HAP_2，HAP_4，HAP_5对红细胞超微结构的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。