法

During the writing progress, the author use all kinds of ways to prove her point,such as quoting,investigating,example setting,antitheses,And massive data and information are involved.

在写作过程中，笔者综合运用了文献法、调查法、案例法及对比分析法，通过大量具有说服力的数据来进行说明论证。

In the present study, mitochondrial 12S, 16S and nuclear 18S, 28S rDNA fragments of ten species of spiders were amplified and sequenced. Using neighbor-joining, maximum parsimony, maximum likelihood methods and Bayesian analyses, the phylogenetic relationship between Deinopoidea and Araneoidea was inferred from the combined DNA sequences of 12S, 16S, 18S and 28S rDNA fragments. The resulting phylogeny indicated that: Deinopoidea and Araneoidea did not form single clade.

本文首先测定了包括复杂生殖器类中妖面蛛总科、园蛛总科和非圆网蛛类等3个类群在内的9科10种蜘蛛线粒体12S rDNA、16S rDNA及核18S rDNA、28S rDNA等4个基因片段序列，并基于4个基因序列的整合数据，分别通过邻接法、最大简约法、最大似然法和贝叶斯法分析，对园蛛总科和妖面蛛总科蜘蛛之间的分子系统关系进行了探讨。

Five methods namely improved CTAB method, CTAB-silica extraction method, SDS method, low pH extraction method with high salt and Zhou shiliang method were adopted to extract genome DNA from the leaves of Citrus aurantium. And three methods including ultraviolet spectrophotometer, agarose gel electrophoresis and SSR-PCR amplification were used to detect extracted sample DNA.

以枳壳叶片为试验材料，分别采用改良CTAB法、CTAB-硅珠法、SDS法、高盐低pH值法和周世良法5种方法提取枳壳基因组DNA，并通过紫外分光光度计、琼脂糖凝胶电泳及SSR-PCR扩增3种方法对所提取的DNA样品进行检测。

Calcium pimelate, calcium azelate and zinc azelate are prepared by alkali neutralization process, and calcium dimer are prepared using double decomposition reaction, the effect of binary carboxylate heat stabilizer on PVC thermal stability is studied adopting Congo red method and toast with heat method.

通过碱中和法制备了庚二酸钙、壬二酸钙、壬二酸锌，采用复分解反应法制备了二聚酸钙，采用刚果红法与热烘法研究了二元羧酸盐热稳定剂对PVC的热稳定作用。

Methods: Selected 93 confirmed cases with hypospadias during May 2003 to Sep 2009. Patients in balanic type were treated with meatal advancement and glandularp lasty; patients with urethra orifice at 2/3 of corpus penis accompanied with slight chordee of penis were treated with Snodgrass method or Onlay method; patients with severe chordee of penis were treated with Duckett method; patients in scrotum or perineum type were treated with combination of Duplay and Duckett; severe hypospadia patients were treated with curved pedicle penis-scrotum and skin flap urethroplasty; patients with good outer plate of ventral prepuce and chordee of penis were treated with ventral pedicle hooded foreskin flap.

选取2003年5月～2008年9月期间收治的93例确诊尿道下裂患者，对阴茎头型患者采用尿道口前移、阴茎头成形法；尿道口在阴茎体外2/3段伴有阴茎轻度下弯者采用尿道板纵切卷管法或加盖岛状皮板法（Snodgrass术或On-lay术）；有严重阴茎下弯的所有阴茎体型采用横裁或纵裁包皮岛状皮瓣尿道成形；阴囊型或会阴型用阴囊中缝皮管加横裁包皮岛状皮瓣成形尿道（Duplay联合Duckett术）；对重度尿道下裂患儿采用弧形带蒂阴茎阴囊联合皮瓣尿道成形术；对腹侧包皮外板发育良好患儿及合并阴茎下曲的患儿采用腹侧带蒂帽状包皮皮瓣法。

Methods: Selected 93 confirmed cases with hypospadias during May 2003 to Sep 2009. Patients in balanic type were treated with meatal advancement and glandularp lasty; patients with urethra orifice at 2/3 of corpus penis accompanied with slight chordee of penis were treated with Snodgrass method or Onlay method; patients with severe chordee of penis were treated with Duckett method; patients in scrotum or perineum type were treated with combination of Duplay and Duckett; severe hypospadia patients were treated with curved pedicle penisscrotum and skin flap urethroplasty; patients with good outer plate of ventral prepuce and chordee of penis were treated with ventral pedicle hooded foreskin flap.

选取2003年5月～2008年9月期间收治的93例确诊尿道下裂患者，对阴茎头型患者采用尿道口前移、阴茎头成形法；尿道口在阴茎体外2/3段伴有阴茎轻度下弯者采用尿道板纵切卷管法或加盖岛状皮板法(Snodgrass术或Onlay术)；有严重阴茎下弯的所有阴茎体型采用横裁或纵裁包皮岛状皮瓣尿道成形；阴囊型或会阴型用阴囊中缝皮管加横裁包皮岛状皮瓣成形尿道(Duplay联合Duckett术)；对重度尿道下裂患儿采用弧形带蒂阴茎阴囊联合皮瓣尿道成形术；对腹侧包皮外板发育良好患儿及合并阴茎下曲的患儿采用腹侧带蒂帽状包皮皮瓣法。

A single dynamic temperature ramp test is adequate in determining both the binodal and spinodal phase separation temperatures.(3) The critical composition and temperature of PMMA/SAN is 56/44 and 184℃, respectively.

常规的研究方法，如差示扫描量热法、动态力学分析法、荧光光谱法、傅立叶转换红外光谱法、小角X射线散射、小角中子散射和小角激光散射等，都存在不可避免的缺陷。

After Bel-7402 was treated with melittin, detection methods were applied as followed, 1 measure the change of proliferation by MTT ; 2 light microscope and transmission electron microscope were used to observe the morphous and biological behaviour and ConA agglutination test and clonality test were applied to measure the adhesion capability and growth potency of Bel-7402; 3 ELISA , colorimetry , bromocresol green mehod were used to determine AFP level, the activity of y - GT and ALP, ALb level respectively; 4 the change of c-myc gene expression was detect by immunohistochemical method.

观察蜂毒素作用于人肝癌Bel-7402细胞株后(1)MTT方法测定对肿瘤细胞增殖的影响(2)光学显微镜和透射电镜下观察对形态及生物学行为的影响，ConA凝集试验及克隆形成能力反映处理后细胞粘附能力及生长潜能(3)ELISA法测定甲胎蛋白含量、重氮反应比色法测定γ-谷氨酰转肽酶活力、比色法测定碱性磷酸酶活力、溴甲酚绿法测定白蛋白含量，检测对肝癌细胞代谢与分泌的影响(4)免疫组织化学法检测处理前后c-myc基因表达的差别。

Serum alt,ast,and bile acid were observed by enzyme assay and the ratio of ast/alt was worked out. serum bilirubin was measured by peariman and lee method. serum albumin was tested by bromphenol assay and was simultaneously measured to hyaluronic acid, type ⅲ procollagen, laminin and type ⅳ collagen by ria. meanwhile, prothrombin time and platelet count were measured and so on. results: the level of serum aca was raised increasingly in different cirrhotic cases with different classifications of child-pugh, according to severity of the condition.

应用酶联免疫吸附法分别对不同分级的肝硬化患者血清中抗心磷脂抗体的含量进行了测定；用酶法测定了alt、ast与胆汁酸并计算ast/alt值；用peariman和lee改良法测定血清胆红素；用溴甲酚氯比色法测定血清白蛋白；用放射免疫分析法测定透明质酸、ⅲ型前胶原氮端肽、层粘连蛋白与ⅳ型胶原；并测定了凝血酶原时间与血小板计数等相关指标。

Caesalpinia Sappan L. that had immunosuppressive function was investigated in this paper. On the basis of establishing the method of determining content of hydroxybenzene in Caesalpinia Sappan L., extractive technology was investigated by orthogonal design. Permeation, soxhlet and ultrasonic extraction were surveyed with water, thirty-five, sixty-five and ninety-five percent of ethanol as solvents.

本文以具有免疫抑制作用的中药苏木为研究对象，在建立苏木中总酚含量分光光度测定方法的基础上，以水、35%、65%、95%乙醇为溶剂，通过渗漉法、煎煮法、索氏法、超声波法对苏木的提取工艺进行了优选。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。